FastQCFastQC Report
Wed 25 May 2016
SRR2088119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839313
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT246620.8685903949300413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT243520.8576722608602856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT193190.680411071269705No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG129180.4549692126229126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103400.36417260090733217No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA72320.2547095019112018No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT71710.25256109488457246No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT64990.228893397804328No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA57640.2030068541228107No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA55200.19441322601629338No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG52520.18497432301405303No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT47510.1673292095658351No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT46040.16215190082953165No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA39120.1377798080028514No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37540.13221508160600823No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31450.11076623112703672No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30870.10872348346237276No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT30710.1081599668652241No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28890.1017499655726579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57950.027.6462461
GTATTGG8900.018.2921351
GTATCAA93450.017.124132
ATTGGAC8550.016.6608183
CAATGCG1251.6601189E-716.2819
ACGTTTA1403.4788172E-815.85714326
CGCGTAA700.002593752215.85714310
TATACCG1557.221388E-915.51612955
ACGGACC2650.015.3584918
TTGGACC13050.014.8850574
GACGGAC2850.014.2807027
TACGTTA1304.449219E-614.23076919
TGGACCC14950.014.1070235
GGACCCT14650.014.0170646
TGCGTAA800.006301630313.87510
CGTCTGT7700.013.69480634
GTACTAG2950.013.1694921
GTCGCGT850.009409185513.0588238
CGCATTG1153.5800537E-412.86956514
TATTGGA12650.012.8695652
Produced by FastQC (version 0.11.5)