##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088119_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2839313
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60571694631765	31.0	31.0	34.0	30.0	34.0
2	31.792362096042247	31.0	31.0	34.0	30.0	34.0
3	31.89088064612813	31.0	31.0	34.0	30.0	34.0
4	35.599865178654134	37.0	35.0	37.0	33.0	37.0
5	35.48045143314597	37.0	35.0	37.0	33.0	37.0
6	35.488444211680786	37.0	35.0	37.0	33.0	37.0
7	35.372437276200266	37.0	35.0	37.0	33.0	37.0
8	35.40247059764105	37.0	35.0	37.0	33.0	37.0
9	36.93389316359274	39.0	37.0	39.0	33.0	39.0
10	36.77915925436893	39.0	37.0	39.0	32.0	39.0
11	36.83903218842023	39.0	37.0	39.0	32.0	39.0
12	36.73304880441149	39.0	35.0	39.0	32.0	39.0
13	36.74329846691788	39.0	35.0	39.0	32.0	39.0
14	37.78214060936572	40.0	37.0	41.0	32.0	41.0
15	37.790967744662176	40.0	37.0	41.0	32.0	41.0
16	37.676856338135316	39.0	37.0	41.0	32.0	41.0
17	37.66204958734736	39.0	37.0	41.0	32.0	41.0
18	37.63748026371168	39.0	37.0	41.0	32.0	41.0
19	37.66649045033077	39.0	37.0	41.0	32.0	41.0
20	37.572785388578154	39.0	36.0	41.0	32.0	41.0
21	37.487571817548826	39.0	36.0	41.0	32.0	41.0
22	37.37854720490485	39.0	36.0	41.0	32.0	41.0
23	37.17241706004234	39.0	36.0	41.0	31.0	41.0
24	37.02421712576246	39.0	36.0	41.0	31.0	41.0
25	36.917718124067335	39.0	36.0	40.0	30.0	41.0
26	37.06959077776913	39.0	36.0	41.0	31.0	41.0
27	36.977325148724354	39.0	36.0	41.0	31.0	41.0
28	36.89951055061559	39.0	36.0	41.0	30.0	41.0
29	36.83541265087717	39.0	35.0	41.0	30.0	41.0
30	36.652931536607625	39.0	35.0	40.0	30.0	41.0
31	36.5009606196992	39.0	35.0	40.0	30.0	41.0
32	36.35362744438532	38.0	35.0	40.0	30.0	41.0
33	36.160202133403395	38.0	35.0	40.0	30.0	41.0
34	36.0465239302606	38.0	35.0	40.0	29.0	41.0
35	35.855508357127235	38.0	35.0	40.0	28.0	41.0
36	35.773585723025256	38.0	35.0	40.0	27.0	41.0
37	35.63037361502589	38.0	35.0	40.0	27.0	41.0
38	35.531070368078474	38.0	34.0	40.0	26.0	41.0
39	35.44084255592814	38.0	34.0	40.0	26.0	41.0
40	35.274591424052225	38.0	34.0	40.0	25.0	41.0
41	35.13946542702407	38.0	34.0	40.0	24.0	41.0
42	34.97168716516988	38.0	34.0	40.0	24.0	41.0
43	34.6559984756876	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	7.0
14	8.0
15	14.0
16	58.0
17	116.0
18	228.0
19	500.0
20	1113.0
21	2180.0
22	3926.0
23	6554.0
24	10565.0
25	15967.0
26	23478.0
27	33036.0
28	44864.0
29	59234.0
30	75815.0
31	94356.0
32	116857.0
33	144941.0
34	178485.0
35	220254.0
36	282825.0
37	385331.0
38	536188.0
39	602407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54072235079401	22.35223802377547	13.467412715681576	23.63962690974894
2	18.27177912403458	22.490440469226183	35.617031302994775	23.620749103744462
3	19.61721021951437	25.366347422774453	31.460779420937392	23.555662936773793
4	13.75283387213738	15.974674155332647	35.36781608790577	34.904675884624204
5	12.052739518327146	39.85196418992904	34.52063932366738	13.574656968076432
6	32.49817825650078	36.66393948113505	15.263234451432442	15.57464781093173
7	26.157912142831734	33.80448016826605	21.40884080057394	18.62876688832827
8	27.459212844797314	34.64517649163724	19.798521684646957	18.097088978918492
9	25.746862004999098	14.126304496897665	22.07872115543443	38.0481123426688
10	16.438870952233867	27.827823138907192	33.5451568742157	22.18814903464324
11	33.02182605440119	22.079848188628727	23.65808207830556	21.240243678664523
12	20.105426911369054	27.09222266090424	31.324126646128835	21.478223781597872
13	30.666221018957756	20.95175840071172	27.22383900612578	21.158181574204747
14	21.054670619266	22.232772505179952	27.345417711960607	29.367139163593446
15	23.627229544611673	29.037904591709335	25.107129788086063	22.227736075592933
16	22.70714782061717	28.238028001844107	25.79278860766671	23.26203556987201
17	20.668450431495224	27.257438683230767	27.258354397701133	24.815756487572873
18	21.592335892520477	26.650813066400218	30.01669770116926	21.740153339910044
19	21.345233864670785	25.40184896839482	30.804564343557754	22.44835282337664
20	24.50124378678927	24.723868062450318	29.863526846106787	20.911361304653624
21	23.83213826724986	25.401038913286417	28.8576497201964	21.909173099267324
22	22.771071734606224	25.045636039422213	29.760579407765185	22.422712818206374
23	22.00440740418545	26.38416405658693	30.386822446133976	21.224606093093644
24	21.317691990985143	25.766092008876797	29.28521793828296	23.630998061855106
25	22.09189335589278	26.41265686453026	29.9294935077605	21.565956271816457
26	23.01486310244767	25.797543278955153	28.988103812436318	22.19948980616086
27	22.019833671032394	26.836245246649455	28.69669529213581	22.447225790182344
28	21.956367614278523	26.244235841557444	29.030156238498538	22.769240305665488
29	21.207207518156682	26.17710692692211	29.92491493540867	22.690770619512538
30	20.83025013445154	26.42963280201936	30.88972578930185	21.850391274227253
31	21.782417084696192	25.98043963451722	30.211709663570023	22.025433617216557
32	22.49568821753713	26.04939997809329	28.920481820778477	22.5344299835911
33	23.054238824673433	25.91813583074497	28.780694484898284	22.24693085968331
34	22.005287898868495	27.511408569608207	28.95182743149487	21.53147610002842
35	21.179982622556935	28.02818850898087	29.10302597846733	21.688802889994868
36	21.555143797108666	25.66553951607308	30.529039947339378	22.25027673947888
37	21.723388721144868	25.744150081375317	30.110206236508617	22.42225496097119
38	21.74082251586916	25.372123467895225	30.751171145977917	22.1358828702577
39	22.375588742769818	25.0587025805186	30.820659786363812	21.74504889034777
40	21.315931001619052	25.379871821106022	30.277112808626594	23.02708436864833
41	21.702362508113758	25.205780412374402	30.217837906564014	22.874019172947822
42	20.26145056920459	26.30446167787771	30.44570993053601	22.988377822381683
43	20.599630967068443	26.021611565896396	30.155498883004444	23.223258584030717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	206.0
1	319.0
2	432.0
3	816.0
4	1200.0
5	1200.0
6	2139.5
7	3079.0
8	3468.0
9	3857.0
10	6200.0
11	8543.0
12	8543.0
13	16708.0
14	24873.0
15	37787.5
16	50702.0
17	48436.0
18	46170.0
19	46170.0
20	51963.5
21	57757.0
22	49668.0
23	41579.0
24	47544.5
25	53510.0
26	53510.0
27	59947.5
28	66385.0
29	72155.5
30	77926.0
31	85635.5
32	93345.0
33	93345.0
34	102425.0
35	111505.0
36	121160.0
37	130815.0
38	137011.5
39	143208.0
40	143208.0
41	149414.5
42	155621.0
43	169528.5
44	183436.0
45	217694.5
46	251953.0
47	251953.0
48	255111.0
49	258269.0
50	240004.0
51	221739.0
52	210213.5
53	198688.0
54	198688.0
55	180850.5
56	163013.0
57	143716.0
58	124419.0
59	114128.0
60	103837.0
61	103837.0
62	91837.0
63	79837.0
64	69555.0
65	59273.0
66	50773.5
67	42274.0
68	42274.0
69	36357.0
70	30440.0
71	25626.0
72	20812.0
73	16878.5
74	12945.0
75	12945.0
76	10100.5
77	7256.0
78	5765.5
79	4275.0
80	3503.0
81	2731.0
82	2731.0
83	2073.0
84	1415.0
85	1090.0
86	765.0
87	645.0
88	525.0
89	525.0
90	487.0
91	449.0
92	291.5
93	134.0
94	103.0
95	72.0
96	72.0
97	49.0
98	26.0
99	21.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2839313.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.62772831971315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.78202739321583	37.60889866987574
2	13.24607732894857	13.147454539659423
3	4.838815996721183	7.204183370230825
4	2.0930796673408367	4.154991563292265
5	1.1398206028740892	2.828335360632347
6	0.652976851009093	1.9443454656564645
7	0.4147880578275229	1.4409492330888043
8	0.29762892270357305	1.1816517852817463
9	0.21014502885941905	0.9386118359976174
>10	1.1515557364553355	10.678362325128795
>50	0.10111014020580894	3.4611257472440276
>100	0.06273659465012368	5.763572827646541
>500	0.004689898665106441	1.5914037698587333
>1k	0.003766130746197943	3.505905493019911
>5k	4.263544240978803E-4	1.3202511652820415
>10k+	3.5529535341490026E-4	3.229956848104799
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24662	0.8685903949300413	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24352	0.8576722608602856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19319	0.680411071269705	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12918	0.4549692126229126	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10340	0.36417260090733217	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7232	0.2547095019112018	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7171	0.25256109488457246	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6499	0.228893397804328	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5764	0.2030068541228107	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5520	0.19441322601629338	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5252	0.18497432301405303	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4751	0.1673292095658351	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4604	0.16215190082953165	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3912	0.1377798080028514	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3754	0.13221508160600823	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3145	0.11076623112703672	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3087	0.10872348346237276	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3071	0.1081599668652241	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2889	0.1017499655726579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.521978732179228E-5	0.0
3	0.0	0.0	0.0	3.521978732179228E-5	0.0
4	0.0	0.0	0.0	3.521978732179228E-5	0.0
5	0.0	0.0	0.0	3.521978732179228E-5	0.0
6	3.521978732179228E-5	0.0	0.0	3.521978732179228E-5	0.0
7	3.521978732179228E-5	0.0	0.0	7.043957464358456E-5	0.0
8	3.521978732179228E-5	0.0	0.0	7.043957464358456E-5	0.0
9	3.521978732179228E-5	0.0	0.0	1.0565936196537683E-4	0.0
10	3.521978732179228E-5	0.0	0.0	2.4653851125254597E-4	0.0
11	3.521978732179228E-5	7.043957464358456E-5	0.0	3.874176605397151E-4	0.0
12	3.521978732179228E-5	7.043957464358456E-5	0.0	7.043957464358456E-4	0.0
13	3.521978732179228E-5	7.043957464358456E-5	0.0	9.157144703665993E-4	0.0
14	3.521978732179228E-5	1.0565936196537683E-4	0.0	0.0011974727689409374	0.0
15	3.521978732179228E-5	1.0565936196537683E-4	0.0	0.0017962091534114062	0.0
16	7.043957464358456E-5	1.0565936196537683E-4	0.0	0.002535824687169044	0.0
17	7.043957464358456E-5	1.0565936196537683E-4	0.0	0.003979835967362528	0.0
18	7.043957464358456E-5	1.0565936196537683E-4	0.0	0.0047898910757637496	0.0
19	7.043957464358456E-5	1.0565936196537683E-4	0.0	0.005388627460234219	0.0
20	7.043957464358456E-5	1.0565936196537683E-4	0.0	0.007043957464358455	0.0
21	7.043957464358456E-5	1.0565936196537683E-4	0.0	0.009967199812067215	0.0
22	1.0565936196537683E-4	1.408791492871691E-4	0.0	0.017469014511608972	0.0
23	1.0565936196537683E-4	2.1131872393075367E-4	0.0	0.0273657747490326	0.0
24	1.0565936196537683E-4	2.1131872393075367E-4	0.0	0.04029143669613037	0.0
25	1.0565936196537683E-4	2.1131872393075367E-4	0.0	0.04568006415636459	0.0
26	1.0565936196537683E-4	2.1131872393075367E-4	0.0	0.05346363715448068	0.0
27	1.0565936196537683E-4	2.1131872393075367E-4	0.0	0.09629089853778008	0.0
28	1.0565936196537683E-4	2.1131872393075367E-4	0.0	0.16951283637978623	0.0
29	1.0565936196537683E-4	2.4653851125254597E-4	0.0	0.2610490636291244	0.0
30	1.408791492871691E-4	2.4653851125254597E-4	0.0	0.424046239354379	0.0
31	1.408791492871691E-4	2.4653851125254597E-4	0.0	0.657025132488035	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5795	0.0	27.646246	1
GTATTGG	890	0.0	18.292135	1
GTATCAA	9345	0.0	17.12413	2
ATTGGAC	855	0.0	16.660818	3
CAATGCG	125	1.6601189E-7	16.28	19
ACGTTTA	140	3.4788172E-8	15.857143	26
CGCGTAA	70	0.0025937522	15.857143	10
TATACCG	155	7.221388E-9	15.5161295	5
ACGGACC	265	0.0	15.358491	8
TTGGACC	1305	0.0	14.885057	4
GACGGAC	285	0.0	14.280702	7
TACGTTA	130	4.449219E-6	14.230769	19
TGGACCC	1495	0.0	14.107023	5
GGACCCT	1465	0.0	14.017064	6
TGCGTAA	80	0.0063016303	13.875	10
CGTCTGT	770	0.0	13.694806	34
GTACTAG	295	0.0	13.169492	1
GTCGCGT	85	0.0094091855	13.058823	8
CGCATTG	115	3.5800537E-4	12.869565	14
TATTGGA	1265	0.0	12.869565	2
>>END_MODULE