FastQCFastQC Report
Wed 25 May 2016
SRR2088118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences999455
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121931.2199648808600687No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114001.1406216387931423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93530.9358100164589701No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG71290.7132887423645886No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48040.48066196076861895No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39950.3997178462261932No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT39300.393214301794478No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT34920.3493904177776888No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA29850.2986627712103096No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA28420.284354973460536No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG27640.27655072014247767No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT25430.2544386690746457No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT25160.2517371967722409No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA21650.21661805684097835No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA20760.20771320369601434No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT16540.16549019215472432No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC15820.15828626601497817No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA15490.15498446653426118No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14770.14778054039451502No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG14360.14367830467604845No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14130.14137705049251842No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT13490.13497356059052182No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13130.13137159752064875No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC11740.1174640178897499No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG11710.11716385430059383No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11460.11466249105762641No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.11386205481987684No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC11290.11296156405240856No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC11260.11266140046325247No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA11210.11216112781465899No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG10470.10475709261547543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26400.030.9734861
ATTGGAC4250.025.6823543
TATACCG601.3371155E-624.6666685
GCGTTAT400.001931011423.1250021
GTATTGG4500.023.0222231
TTGGACC6500.020.4923084
GGACCCT6600.019.9015166
CGTCTGT2950.018.81355934
TATTGGA6050.018.6528932
GTATCAA43800.018.5422362
ACGGACC701.2189952E-418.58
TCTACGC500.007034256618.4999983
GACCCTC6550.018.358787
TGGACCC6900.018.2318845
GACCGTG752.066926E-417.2666667
AGGCGGG752.066926E-417.26666629
GTTCGCT752.066926E-417.26666637
TACTATA1401.8681021E-917.1785722
GTTCTAA1005.876247E-616.651
TATAGAC904.4450237E-516.4444453
Produced by FastQC (version 0.11.5)