##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088118_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	999455
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.573902777013473	31.0	31.0	34.0	30.0	34.0
2	31.756320194506007	31.0	31.0	34.0	30.0	34.0
3	31.837246299233083	31.0	31.0	34.0	30.0	34.0
4	35.558125178222134	37.0	35.0	37.0	33.0	37.0
5	35.44589901496315	37.0	35.0	37.0	33.0	37.0
6	35.47684688155044	37.0	35.0	37.0	33.0	37.0
7	35.32891025608957	37.0	35.0	37.0	32.0	37.0
8	35.36815764591702	37.0	35.0	37.0	33.0	37.0
9	36.87341701227169	39.0	37.0	39.0	33.0	39.0
10	36.74213946600897	39.0	35.0	39.0	32.0	39.0
11	36.827501988583776	39.0	37.0	39.0	32.0	39.0
12	36.7106883251372	39.0	35.0	39.0	32.0	39.0
13	36.72443881915644	39.0	35.0	39.0	32.0	39.0
14	37.710297111925996	39.0	37.0	41.0	32.0	41.0
15	37.7177661825695	39.0	37.0	41.0	32.0	41.0
16	37.593315356869496	39.0	36.0	41.0	32.0	41.0
17	37.63450380457349	39.0	36.0	41.0	32.0	41.0
18	37.62674057361262	39.0	36.0	41.0	32.0	41.0
19	37.65505000225123	39.0	37.0	41.0	32.0	41.0
20	37.57434801967072	39.0	36.0	41.0	32.0	41.0
21	37.49156390232677	39.0	36.0	41.0	32.0	41.0
22	37.40779224677449	39.0	36.0	41.0	32.0	41.0
23	37.1728612093591	39.0	36.0	41.0	31.0	41.0
24	37.033547283269385	39.0	36.0	41.0	31.0	41.0
25	36.93203696014328	39.0	36.0	40.0	31.0	41.0
26	37.01521729342492	39.0	36.0	41.0	31.0	41.0
27	36.924440820247035	39.0	36.0	41.0	31.0	41.0
28	36.81260186801807	39.0	35.0	41.0	30.0	41.0
29	36.696858788039485	39.0	35.0	40.0	30.0	41.0
30	36.52920441640694	39.0	35.0	40.0	30.0	41.0
31	36.36772741143923	38.0	35.0	40.0	30.0	41.0
32	36.204804618517095	38.0	35.0	40.0	30.0	41.0
33	36.00080143678305	38.0	35.0	40.0	29.0	41.0
34	35.841123412259684	38.0	35.0	40.0	29.0	41.0
35	35.63681906639118	38.0	34.0	40.0	27.0	41.0
36	35.52123607366015	38.0	34.0	40.0	27.0	41.0
37	35.37228589581322	38.0	34.0	40.0	26.0	41.0
38	35.24781706029786	38.0	34.0	40.0	25.0	41.0
39	35.14001931052424	38.0	34.0	40.0	25.0	41.0
40	34.921664307047344	38.0	34.0	40.0	24.0	41.0
41	34.77582782616526	38.0	33.0	40.0	23.0	41.0
42	34.56859688530249	38.0	33.0	40.0	23.0	41.0
43	34.23741439084301	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	5.0
14	5.0
15	4.0
16	17.0
17	42.0
18	77.0
19	169.0
20	354.0
21	710.0
22	1319.0
23	2342.0
24	3826.0
25	5927.0
26	8718.0
27	12096.0
28	16612.0
29	22000.0
30	27893.0
31	34547.0
32	42890.0
33	52762.0
34	65606.0
35	79679.0
36	101997.0
37	138659.0
38	180996.0
39	200199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.5699106012777	22.761104802117156	13.844245113586906	22.824739483018245
2	18.168501833499256	22.354783356929527	36.08446603398852	23.39224877558269
3	19.911251632139514	25.56633365184025	31.93740588620798	22.585008829812246
4	13.378291168686934	15.68254698810852	35.01208158446353	35.927080258741015
5	11.349485469580923	40.53559189758418	34.44817425496896	13.666748377865936
6	31.98963435072114	35.994116793652545	15.256614855096027	16.759634000530287
7	25.271773116348413	34.79656412744946	21.031862364988918	18.899800391213212
8	28.02067126583988	34.25316797654722	19.724249716095272	18.001911041517626
9	25.436562926795105	13.849347894602559	23.056865991965623	37.65722318663672
10	16.644771400413223	27.858182709576717	33.07142392603969	22.425621963970364
11	31.95611608326538	21.56465273574098	24.56008524645932	21.91914593453432
12	19.827906208883842	27.334097082910187	31.519578170102708	21.318418538103266
13	31.14517411989534	20.732098993951702	28.451305961749153	19.6714209244038
14	20.779024568389772	22.31606225392839	27.871990234677902	29.032922943003936
15	23.411459245288682	28.547958637457416	26.463322510768368	21.577259606485537
16	22.23481797579681	28.277711352687213	26.48373363483098	23.003737036684992
17	19.778479271202805	26.853235013082127	27.68078602838547	25.687499687329595
18	20.96162408512639	26.376875397091414	31.48165750333932	21.17984301444287
19	20.380907594639076	24.59680525886608	32.513319759268796	22.50896738722604
20	24.843739838211825	23.653291043618772	31.478956031036915	20.024013087132488
21	23.825885107383524	24.8318333491753	30.207563121901437	21.134718421539738
22	22.383098788839916	23.974165920426632	31.40111360691577	22.24162168381768
23	21.26899160042223	26.03328814203741	32.51211910491218	20.185601152628184
24	20.245133597810806	24.975611708381066	30.75686249005708	24.022392203751046
25	21.14182229314977	26.173964810821897	31.874871805133797	20.809341090894538
26	22.767108073900275	25.11668859528443	30.443791866567278	21.672411464248015
27	21.462797224487346	26.667233642335077	29.81665007429049	22.053319058887094
28	21.209659264299045	25.864195986812817	30.273098838867185	22.65304591002096
29	20.220119965381134	25.75743780360296	31.490662411014004	22.5317798200019
30	19.67842474148411	25.79305721618282	33.20609732304106	21.322420719292015
31	20.9413130155935	25.259366354663292	32.19964880860069	21.599671821142522
32	22.142067426747577	25.399642805328902	30.18194916229345	22.276340605630068
33	23.177031482157776	25.20433636331801	29.8894897719257	21.729142382598514
34	21.30431084941293	28.049587024928584	29.79954074970859	20.846561375949893
35	20.140676668784486	28.629302970118715	30.149231331075438	21.080789030021364
36	20.93050712638388	24.98921912442281	32.34602858557914	21.73424516361417
37	21.06628112321215	25.29628647612949	31.53548684032798	22.10194556033038
38	21.187247049642053	24.68435297236994	32.43057466319144	21.697825314796564
39	22.239920756812463	24.39289412729938	32.236668984596605	21.130516131291554
40	20.821847907109376	24.984916779644905	31.184895768193666	23.008339545052053
41	21.551345483288394	24.74048356354213	31.099849417932774	22.6083215352367
42	19.253693262828243	26.464723274184433	31.418322986027388	22.863260476959944
43	19.906549069242736	26.191774517111828	31.036815064209993	22.864861349435444
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	89.5
2	135.0
3	288.0
4	441.0
5	441.0
6	849.5
7	1258.0
8	1385.5
9	1513.0
10	2526.5
11	3540.0
12	3540.0
13	7266.5
14	10993.0
15	17009.5
16	23026.0
17	21864.5
18	20703.0
19	20703.0
20	22972.5
21	25242.0
22	20275.0
23	15308.0
24	16801.5
25	18295.0
26	18295.0
27	20028.0
28	21761.0
29	23039.5
30	24318.0
31	26482.5
32	28647.0
33	28647.0
34	31712.0
35	34777.0
36	38329.0
37	41881.0
38	44285.5
39	46690.0
40	46690.0
41	49366.0
42	52042.0
43	59093.5
44	66145.0
45	83867.0
46	101589.0
47	101589.0
48	102656.0
49	103723.0
50	94085.0
51	84447.0
52	79846.0
53	75245.0
54	75245.0
55	65427.5
56	55610.0
57	47110.5
58	38611.0
59	34337.5
60	30064.0
61	30064.0
62	26527.5
63	22991.0
64	20001.5
65	17012.0
66	14282.5
67	11553.0
68	11553.0
69	9748.0
70	7943.0
71	6796.0
72	5649.0
73	4597.5
74	3546.0
75	3546.0
76	2765.5
77	1985.0
78	1557.0
79	1129.0
80	916.5
81	704.0
82	704.0
83	532.0
84	360.0
85	275.0
86	190.0
87	164.0
88	138.0
89	138.0
90	134.5
91	131.0
92	84.0
93	37.0
94	30.0
95	23.0
96	23.0
97	14.0
98	5.0
99	8.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	999455.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.4297570117891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.88236244030169	41.59760246844173
2	10.943483347371695	11.256413788357735
3	3.3198361644813676	5.122151017746799
4	1.4816038100840356	3.047940957614515
5	0.7822917501249216	2.011653731062598
6	0.4829119024157112	1.4901625079604504
7	0.32139327202517914	1.1570424519835159
8	0.23634584367943326	0.9724167448943636
9	0.18844540953021355	0.8722531459913372
>10	1.194216342445046	11.718312700775275
>50	0.09733670053835657	3.4577166832672717
>100	0.056873803875874616	5.429856316194607
>500	0.006840491809528965	2.316283011288691
>1k	0.0052769508244937735	5.5220291525415215
>5k	3.90885246258798E-4	1.6566992098620121
>10k+	3.90885246258798E-4	2.371466112017622
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12193	1.2199648808600687	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11400	1.1406216387931423	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9353	0.9358100164589701	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7129	0.7132887423645886	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4804	0.48066196076861895	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3995	0.3997178462261932	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3930	0.393214301794478	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3492	0.3493904177776888	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2985	0.2986627712103096	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2842	0.284354973460536	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2764	0.27655072014247767	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2543	0.2544386690746457	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2516	0.2517371967722409	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2165	0.21661805684097835	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2076	0.20771320369601434	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1654	0.16549019215472432	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1582	0.15828626601497817	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1549	0.15498446653426118	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1477	0.14778054039451502	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1436	0.14367830467604845	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1413	0.14137705049251842	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1349	0.13497356059052182	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1313	0.13137159752064875	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1174	0.1174640178897499	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1171	0.11716385430059383	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1146	0.11466249105762641	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1138	0.11386205481987684	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1129	0.11296156405240856	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1126	0.11266140046325247	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1121	0.11216112781465899	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1047	0.10475709261547543	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0005452971869668E-4	0.0
5	0.0	0.0	0.0	1.0005452971869668E-4	0.0
6	0.0	0.0	0.0	1.0005452971869668E-4	0.0
7	0.0	0.0	0.0	2.0010905943739337E-4	0.0
8	0.0	0.0	0.0	2.0010905943739337E-4	0.0
9	0.0	0.0	0.0	2.0010905943739337E-4	0.0
10	0.0	0.0	0.0	2.0010905943739337E-4	0.0
11	0.0	0.0	0.0	7.003817080308768E-4	0.0
12	0.0	0.0	0.0	0.001300708886343057	0.0
13	0.0	0.0	0.0	0.0017009270052178438	0.0
14	0.0	0.0	0.0	0.002201199653811327	0.0
15	0.0	0.0	0.0	0.0031016904212795974	0.0
16	0.0	0.0	0.0	0.005703108193965712	0.0
17	0.0	0.0	0.0	0.0078042533180583415	0.0
18	0.0	0.0	0.0	0.009505180323276186	0.0
19	0.0	0.0	0.0	0.01150627091765012	1.0005452971869668E-4
20	0.0	0.0	0.0	0.01420774322005493	1.0005452971869668E-4
21	0.0	0.0	0.0	0.018410033468240192	1.0005452971869668E-4
22	0.0	0.0	0.0	0.03241766762885773	1.0005452971869668E-4
23	0.0	0.0	0.0	0.05252862810231576	1.0005452971869668E-4
24	0.0	0.0	0.0	0.07824264224002081	1.0005452971869668E-4
25	0.0	0.0	0.0	0.08564667743920437	1.0005452971869668E-4
26	0.0	0.0	0.0	0.09505180323276186	1.0005452971869668E-4
27	0.0	0.0	0.0	0.1482808130431085	1.0005452971869668E-4
28	0.0	0.0	0.0	0.24293239815699555	1.0005452971869668E-4
29	0.0	0.0	0.0	0.3540929806744676	1.0005452971869668E-4
30	0.0	0.0	0.0	0.572011746401789	1.0005452971869668E-4
31	0.0	0.0	0.0	0.8287516696599647	1.0005452971869668E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2640	0.0	30.973486	1
ATTGGAC	425	0.0	25.682354	3
TATACCG	60	1.3371155E-6	24.666668	5
GCGTTAT	40	0.0019310114	23.125002	1
GTATTGG	450	0.0	23.022223	1
TTGGACC	650	0.0	20.492308	4
GGACCCT	660	0.0	19.901516	6
CGTCTGT	295	0.0	18.813559	34
TATTGGA	605	0.0	18.652893	2
GTATCAA	4380	0.0	18.542236	2
ACGGACC	70	1.2189952E-4	18.5	8
TCTACGC	50	0.0070342566	18.499998	3
GACCCTC	655	0.0	18.35878	7
TGGACCC	690	0.0	18.231884	5
GACCGTG	75	2.066926E-4	17.266666	7
AGGCGGG	75	2.066926E-4	17.266666	29
GTTCGCT	75	2.066926E-4	17.266666	37
TACTATA	140	1.8681021E-9	17.178572	2
GTTCTAA	100	5.876247E-6	16.65	1
TATAGAC	90	4.4450237E-5	16.444445	3
>>END_MODULE