FastQCFastQC Report
Wed 25 May 2016
SRR2088117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974434
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT122261.2546770740758224No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115131.1815063924288356No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77090.7911259254090067No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG46870.48099717374393747No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44970.46149867512833087No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT31240.3205963667113422No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30610.31413107506511473No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT28560.2930932212956444No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA25330.25994577364911325No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25140.2579959237875526No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG21960.22536159452564258No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT19200.19703745969455091No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT18010.1848252421405657No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16150.1657372382326561No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.14090230841698873No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT12860.1319740485245794No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12770.1310504354322612No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12580.12910058557070053No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA11690.11996707832444271No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA10960.1124755499089728No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT10240.10508664517042714No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG10010.10272630060116951No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9800.10057120338576035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGT358.868422E-426.42857214
GGTATCA26550.026.1996231
TAGGGTG951.675453E-719.4736845
GTATTGG2950.019.4406781
ACCGCAA500.00703413218.58
CCGCTGA609.2354394E-418.535
ACGGGTG500.00703413218.515
ATTGGAC2900.018.53
AGTACCG801.6162587E-518.55
TATACCG953.605248E-617.5263165
CGTGCAT650.00157970117.0769233
CGTCTGT1950.017.07692334
ACGAGAC650.00157970117.07692319
GTATCAA41300.016.887412
TAGTACC803.3819038E-416.18754
ACAACGC700.002592113615.85714318
AAAGCCG700.002592113615.85714319
ATAAGAC855.363111E-415.2352933
GTATACT855.363111E-415.2352934
TATTGGA3850.014.8961042
Produced by FastQC (version 0.11.5)