##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088117_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	974434
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.101309067622847	31.0	31.0	33.0	30.0	34.0
2	31.300119864454647	31.0	31.0	34.0	30.0	34.0
3	31.36766061118557	31.0	31.0	34.0	28.0	34.0
4	35.13192786786996	35.0	35.0	37.0	32.0	37.0
5	34.97988575932285	35.0	35.0	37.0	32.0	37.0
6	35.04430058885466	36.0	35.0	37.0	32.0	37.0
7	34.8913389721623	36.0	35.0	37.0	32.0	37.0
8	34.965259832887604	36.0	35.0	37.0	32.0	37.0
9	36.366626164522174	38.0	35.0	39.0	32.0	39.0
10	36.14630441877028	38.0	35.0	39.0	32.0	39.0
11	36.29018076134454	38.0	35.0	39.0	32.0	39.0
12	36.11402208872022	38.0	35.0	39.0	31.0	39.0
13	36.16535342568096	38.0	35.0	39.0	32.0	39.0
14	37.00779529449917	39.0	36.0	40.0	31.0	41.0
15	37.04909516704056	39.0	36.0	40.0	31.0	41.0
16	36.892229745678	38.0	36.0	40.0	31.0	41.0
17	36.91611848519243	38.0	36.0	40.0	31.0	41.0
18	36.8976749579756	38.0	36.0	40.0	31.0	41.0
19	36.934897591832794	39.0	36.0	40.0	31.0	41.0
20	36.870688009654835	39.0	36.0	40.0	31.0	41.0
21	36.786765445376496	39.0	36.0	40.0	30.0	41.0
22	36.666847626416974	38.0	35.0	40.0	30.0	41.0
23	36.41774814918199	38.0	35.0	40.0	30.0	41.0
24	36.26683592731781	38.0	35.0	40.0	29.0	41.0
25	36.14823066518615	38.0	34.0	40.0	29.0	41.0
26	36.250369958355314	38.0	35.0	40.0	30.0	41.0
27	36.12135249796292	38.0	35.0	40.0	29.0	41.0
28	36.02828616407063	38.0	35.0	40.0	29.0	41.0
29	35.93476931223664	38.0	34.0	40.0	29.0	41.0
30	35.708467684830374	38.0	34.0	40.0	27.0	41.0
31	35.51791193657036	38.0	34.0	40.0	27.0	41.0
32	35.35927112559701	38.0	34.0	40.0	27.0	41.0
33	35.14065293288206	38.0	34.0	40.0	26.0	41.0
34	34.98067083045132	38.0	33.0	40.0	25.0	41.0
35	34.74305083771708	38.0	33.0	40.0	25.0	41.0
36	34.668085267960684	37.0	33.0	40.0	25.0	41.0
37	34.489125995193106	37.0	33.0	40.0	24.0	41.0
38	34.36923075344251	37.0	33.0	40.0	24.0	41.0
39	34.24553330446187	37.0	33.0	40.0	23.0	41.0
40	34.03213557819206	37.0	33.0	40.0	22.0	41.0
41	33.86043795680364	37.0	33.0	40.0	21.0	41.0
42	33.64779246208568	37.0	32.0	40.0	20.0	41.0
43	33.30390462565961	37.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	6.0
12	1.0
13	3.0
14	7.0
15	20.0
16	26.0
17	80.0
18	164.0
19	355.0
20	691.0
21	1345.0
22	2189.0
23	3651.0
24	5757.0
25	8468.0
26	12027.0
27	16261.0
28	21398.0
29	27494.0
30	33983.0
31	41626.0
32	49842.0
33	59851.0
34	71424.0
35	86579.0
36	109040.0
37	141394.0
38	174656.0
39	106095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.36473275768292	24.9676222299304	14.863294999969213	21.804350012417466
2	19.603790508130874	24.378664948062156	33.74071512283028	22.27682942097669
3	21.324584322796618	25.93895533201838	31.76972478382322	20.966735561361773
4	15.04114183207893	17.261815577042672	34.1496704753734	33.547372115505006
5	11.556452258439258	41.34328235673222	33.91086517917068	13.189400205657847
6	32.26529451969041	37.47652483390358	14.536746459996264	15.721434186409752
7	24.911692326006687	35.27350236137081	21.00460369814682	18.810201614475687
8	27.96577295127223	34.86403389044307	19.519639093052994	17.650554065231713
9	25.441435746289642	13.902737383958277	22.606867165965063	38.04895970378702
10	16.462685004833574	29.0158184135611	33.016397211098955	21.505099370506368
11	32.816691535804374	22.18446811174487	23.565680179468288	21.433160172982472
12	19.56212529529963	28.1565503666744	31.258556249063556	21.022768088962412
13	31.05320627153814	21.13288329430213	27.834004150101492	19.97990628405823
14	20.59482735618831	22.66413117768879	28.036583288349952	28.704458177772946
15	23.57347957891453	29.683795926661016	25.410751266889292	21.331973227535165
16	22.080612950697535	28.48566449857045	26.341855887622966	23.091866663109045
17	20.199828823706888	27.685097194884417	27.69402545477682	24.421048526631868
18	21.029849122670186	26.431754228608607	31.221406478016984	21.316990170704226
19	20.606218584326903	25.397615436242987	32.32225066038336	21.67391531904675
20	24.05796595767389	24.573239439510527	31.305044774710243	20.06374982810534
21	23.792478505470868	25.02129441296178	30.066171746880755	21.120055334686597
22	22.2959174248846	24.95458902296102	30.82158463272012	21.927908919434255
23	21.377743387443378	26.688723915626916	31.54333695252834	20.390195744401364
24	20.63177187988104	25.863013811094444	30.30251407483729	23.202700234187233
25	21.341209358458347	26.616887341779943	31.01297779018384	21.028925509577867
26	22.450263434978666	25.752282863693182	30.135237481450773	21.662216219877386
27	21.684998676154567	26.94928543133758	29.848712175478276	21.517003717029578
28	21.446090756274923	26.662554878011235	29.98776725771063	21.90358710800321
29	20.59698245340372	26.192333190344343	30.900707487628715	22.30997686862322
30	20.079861745382445	26.617195212810717	32.26508927233655	21.037853769470278
31	21.141093188456068	26.00412136686528	31.44132901766564	21.413456427013013
32	21.8574064533873	26.37469546423873	29.919522512556007	21.848375569817964
33	22.82237688750598	26.214602528236902	29.38988171594998	21.57313886830714
34	21.36614691195094	28.23413386642913	29.78539336681602	20.614325854803916
35	20.45515653189441	28.700661101726745	30.021325200064858	20.822857166313984
36	21.16007856868705	25.853469809140485	31.580691970928765	21.4057596512437
37	20.89797769782253	26.27053243216062	31.15747192729318	21.674017942723673
38	21.303443845350223	25.763571468154844	31.625230646713888	21.30775403978104
39	22.16712471034467	25.403978104212293	31.537692650297505	20.89120453514553
40	20.831682802529468	25.962250906680183	30.84754842298196	22.358517867808388
41	21.243203746995693	25.693171625784817	30.84128837868958	22.222336248529917
42	19.63468023488507	26.88175905192142	30.996455378199038	22.48710533499447
43	20.010898634489354	26.583739894133412	30.77006754690415	22.63529392447308
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	104.0
2	139.0
3	228.5
4	318.0
5	318.0
6	675.5
7	1033.0
8	1239.0
9	1445.0
10	2387.5
11	3330.0
12	3330.0
13	7001.0
14	10672.0
15	17357.5
16	24043.0
17	21798.0
18	19553.0
19	19553.0
20	22153.0
21	24753.0
22	20302.0
23	15851.0
24	17864.5
25	19878.0
26	19878.0
27	22145.5
28	24413.0
29	25657.0
30	26901.0
31	29150.0
32	31399.0
33	31399.0
34	34290.0
35	37181.0
36	40135.5
37	43090.0
38	45325.0
39	47560.0
40	47560.0
41	50041.0
42	52522.0
43	58242.0
44	63962.0
45	77322.0
46	90682.0
47	90682.0
48	92351.5
49	94021.0
50	85976.0
51	77931.0
52	72851.5
53	67772.0
54	67772.0
55	59835.5
56	51899.0
57	45119.0
58	38339.0
59	34454.0
60	30569.0
61	30569.0
62	26998.0
63	23427.0
64	20391.0
65	17355.0
66	14728.5
67	12102.0
68	12102.0
69	10219.0
70	8336.0
71	6986.5
72	5637.0
73	4543.5
74	3450.0
75	3450.0
76	2717.5
77	1985.0
78	1567.0
79	1149.0
80	917.5
81	686.0
82	686.0
83	532.5
84	379.0
85	310.5
86	242.0
87	201.0
88	160.0
89	160.0
90	143.5
91	127.0
92	85.5
93	44.0
94	34.0
95	24.0
96	24.0
97	14.0
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	974434.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.00240299484866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.58149545753238	47.07343683987525
2	10.394011722569426	11.84967289886167
3	3.080698350906439	5.268216267118035
4	1.2318296804795583	2.8086900747084576
5	0.6354923049531225	1.8112294233531574
6	0.3873139464994058	1.324669539833063
7	0.2723144835308666	1.0865805952092376
8	0.1924831558618723	0.8777601936126969
9	0.15967489464936876	0.8191667423666991
>10	0.9321240633906429	10.074121750535733
>50	0.0813442560570442	3.14217866754022
>100	0.04310220570272649	4.795740899551335
>500	0.0041479109098790795	1.6940475714381003
>1k	0.0034265350994653267	4.141621976946306
>5k	1.8034395260343823E-4	0.7924881806066092
>10k+	3.6068790520687647E-4	2.4403783784434165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12226	1.2546770740758224	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11513	1.1815063924288356	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7709	0.7911259254090067	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4687	0.48099717374393747	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4497	0.46149867512833087	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3124	0.3205963667113422	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3061	0.31413107506511473	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2856	0.2930932212956444	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2533	0.25994577364911325	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2514	0.2579959237875526	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2196	0.22536159452564258	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1920	0.19703745969455091	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1801	0.1848252421405657	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1615	0.1657372382326561	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1373	0.14090230841698873	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1286	0.1319740485245794	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1277	0.1310504354322612	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1258	0.12910058557070053	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1169	0.11996707832444271	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1096	0.1124755499089728	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1024	0.10508664517042714	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1001	0.10272630060116951	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	980	0.10057120338576035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.052473538484905E-4	0.0
12	0.0	0.0	0.0	4.10494707696981E-4	0.0
13	0.0	0.0	0.0	4.10494707696981E-4	0.0
14	0.0	0.0	0.0	4.10494707696981E-4	0.0
15	0.0	0.0	0.0	0.0010262367692424525	0.0
16	0.0	0.0	0.0	0.001641978830787924	0.0
17	0.0	0.0	0.0	0.003283957661575848	0.0
18	0.0	0.0	0.0	0.003591828692348584	0.0
19	0.0	0.0	0.0	0.004207570753894056	0.0
20	0.0	0.0	0.0	0.005233807523136508	0.0
21	0.0	0.0	0.0	0.007183657384697168	0.0
22	0.0	0.0	0.0	0.015598798892485279	0.0
23	0.0	0.0	0.0	0.023706069369500652	0.0
24	0.0	0.0	0.0	0.03499467383116763	0.0
25	0.0	0.0	0.0	0.039612739292758666	0.0
26	0.0	0.0	0.0	0.045462288877440644	0.0
27	0.0	0.0	0.0	0.08199631786247195	0.0
28	0.0	0.0	0.0	0.14552037387857977	0.0
29	0.0	0.0	0.0	0.2224881315717637	0.0
30	0.0	0.0	0.0	0.3599012349733281	0.0
31	1.0262367692424525E-4	0.0	0.0	0.5433923693138786	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGGGT	35	8.868422E-4	26.428572	14
GGTATCA	2655	0.0	26.199623	1
TAGGGTG	95	1.675453E-7	19.473684	5
GTATTGG	295	0.0	19.440678	1
ACCGCAA	50	0.007034132	18.5	8
CCGCTGA	60	9.2354394E-4	18.5	35
ACGGGTG	50	0.007034132	18.5	15
ATTGGAC	290	0.0	18.5	3
AGTACCG	80	1.6162587E-5	18.5	5
TATACCG	95	3.605248E-6	17.526316	5
CGTGCAT	65	0.001579701	17.076923	3
CGTCTGT	195	0.0	17.076923	34
ACGAGAC	65	0.001579701	17.076923	19
GTATCAA	4130	0.0	16.88741	2
TAGTACC	80	3.3819038E-4	16.1875	4
ACAACGC	70	0.0025921136	15.857143	18
AAAGCCG	70	0.0025921136	15.857143	19
ATAAGAC	85	5.363111E-4	15.235293	3
GTATACT	85	5.363111E-4	15.235293	4
TATTGGA	385	0.0	14.896104	2
>>END_MODULE