FastQCFastQC Report
Wed 25 May 2016
SRR2088116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521586
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT216490.8585469621103544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200280.7942620239801459No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT155530.6167943508569607No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG115910.4596710165744892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87280.3461313633562369No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT68820.2729234695941364No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA64480.25571207961973136No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT55780.22120998450974902No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG50170.1989620818008983No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA49690.19705851793276136No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA46950.18619234085214623No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT39670.15732162218540235No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT36050.14296557801320278No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32860.13031480980620927No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA31710.12575418803879782No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27150.1076703312914967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51800.030.3571431
GTATCAA81650.019.2363742
TAGGACG1552.1827873E-1117.9032254
TATATCG1258.592906E-917.765
CGAACGA2000.017.57516
GCGCGAC953.6098427E-617.52631625
GTATTGG8350.017.059881
ATTGGAC8250.016.8181823
CGTAATA700.002593644415.8571432
ACGGACC3200.015.6093768
CGAATTA1802.0190782E-1015.41666615
ACGAACG2300.015.28260915
GTATTAG4400.015.1363641
TGGACCC12350.015.1295565
AAGACGG3800.015.0921065
GTTACGA750.004106213314.825
ACGTAGA750.004106213314.87
TTGGACC13250.014.66037754
GGACCCT12850.014.2529186
TAGACTG3400.014.1470595
Produced by FastQC (version 0.11.5)