##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088116_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2521586
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26706564836575	31.0	31.0	33.0	30.0	34.0
2	31.46630731610978	31.0	31.0	34.0	30.0	34.0
3	31.55106270418697	31.0	31.0	34.0	30.0	34.0
4	35.321924772742236	37.0	35.0	37.0	33.0	37.0
5	35.16525789721231	37.0	35.0	37.0	32.0	37.0
6	35.19166072463918	37.0	35.0	37.0	32.0	37.0
7	35.05332992806908	37.0	35.0	37.0	32.0	37.0
8	35.071987233431656	37.0	35.0	37.0	32.0	37.0
9	36.562346475591156	38.0	35.0	39.0	32.0	39.0
10	36.36940639740227	38.0	35.0	39.0	32.0	39.0
11	36.47341990318792	38.0	35.0	39.0	32.0	39.0
12	36.3388220746784	38.0	35.0	39.0	32.0	39.0
13	36.37138650040094	38.0	35.0	39.0	32.0	39.0
14	37.31660312200337	39.0	36.0	40.0	32.0	41.0
15	37.33277905254867	39.0	36.0	41.0	32.0	41.0
16	37.19132641123483	39.0	36.0	40.0	31.0	41.0
17	37.19157387453769	39.0	36.0	40.0	31.0	41.0
18	37.16471141575183	39.0	36.0	40.0	31.0	41.0
19	37.19706565629727	39.0	36.0	40.0	31.0	41.0
20	37.10540865947067	39.0	36.0	40.0	31.0	41.0
21	37.00815161568949	39.0	36.0	40.0	31.0	41.0
22	36.91995712222387	39.0	36.0	40.0	31.0	41.0
23	36.695397658457814	39.0	35.0	40.0	30.0	41.0
24	36.551174935140025	38.0	35.0	40.0	30.0	41.0
25	36.44981967698107	38.0	35.0	40.0	30.0	41.0
26	36.52817314182423	38.0	35.0	40.0	30.0	41.0
27	36.418084887844394	38.0	35.0	40.0	30.0	41.0
28	36.31269843661886	38.0	35.0	40.0	30.0	41.0
29	36.21741237459282	38.0	35.0	40.0	30.0	41.0
30	36.056974856300755	38.0	35.0	40.0	29.0	41.0
31	35.92927268790356	38.0	35.0	40.0	29.0	41.0
32	35.78583756413622	38.0	34.0	40.0	28.0	41.0
33	35.59342175916269	38.0	34.0	40.0	27.0	41.0
34	35.459773333132404	38.0	34.0	40.0	27.0	41.0
35	35.26412305588626	38.0	34.0	40.0	26.0	41.0
36	35.182405041906165	38.0	34.0	40.0	25.0	41.0
37	35.04208859027612	38.0	33.0	40.0	25.0	41.0
38	34.944113744286334	38.0	33.0	40.0	25.0	41.0
39	34.87383139024408	38.0	33.0	40.0	24.0	41.0
40	34.70346004459098	38.0	33.0	40.0	24.0	41.0
41	34.586824324056366	38.0	33.0	40.0	24.0	41.0
42	34.42565274394766	38.0	33.0	40.0	23.0	41.0
43	34.09837816358435	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	5.0
13	7.0
14	21.0
15	18.0
16	63.0
17	146.0
18	292.0
19	640.0
20	1318.0
21	2531.0
22	4632.0
23	7708.0
24	12430.0
25	18298.0
26	26530.0
27	36929.0
28	49723.0
29	64165.0
30	80699.0
31	99231.0
32	120013.0
33	145207.0
34	174976.0
35	212106.0
36	264725.0
37	343937.0
38	460452.0
39	394780.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.781232922454365	23.118981466426288	13.872301004209255	23.227484606910096
2	18.81415109379573	22.814926796071997	34.96212304478213	23.40879906535014
3	20.20914614849543	25.71195271547351	31.181327942017447	22.89757319401361
4	14.290133273265319	16.35363616390637	35.20645339877363	34.14977716405468
5	12.146244466776068	39.996771872940286	34.51145429900071	13.345529361282937
6	32.62982107292791	36.791051346255884	15.129763569436061	15.44936401138014
7	26.09056363732984	33.79349346006838	21.626389105903982	18.48955379669779
8	27.344655308206818	34.79881312792822	19.808723557316704	18.04780800654826
9	25.711119906281205	14.080701590189667	22.140827241267996	38.067351262261134
10	16.43263406443405	27.81483558363665	33.621617505807855	22.130912846121447
11	33.30098596676853	22.10136794858474	23.439771635787952	21.157874448858774
12	19.868606504001846	27.15878022799936	31.304623360059896	21.667989907938892
13	30.37727049563251	20.89657065037639	27.602746842661723	21.12341201132938
14	21.053535354336518	22.181079685562974	27.332083855161	29.433301104939506
15	23.776583467706438	29.061511286944008	25.01397929715663	22.147925948192924
16	22.924342060909282	28.09985461530957	25.776792859731927	23.199010464049213
17	20.8003613598743	27.224968729997705	27.28588277377809	24.6887871363499
18	21.725057166402415	26.450971729697102	29.903322750046996	21.920648353853487
19	21.434684361350357	25.33219172378019	30.678668108087532	22.554455806781924
20	24.410985784343666	24.669632524926772	29.883414644592726	21.035967046136836
21	24.050022485848192	25.173601059016033	28.863976878044216	21.91239957709156
22	22.74635883923848	24.978961653499027	29.860254617530398	22.4144248897321
23	21.92195705401283	26.28516338526626	30.52566916218602	21.267210398534893
24	21.375197990471076	25.671383010533848	29.267135842283388	23.68628315671169
25	22.17572591218384	26.356507372740808	29.80239420745515	21.665372507620205
26	23.081901628578205	25.783217387786895	28.999486830907216	22.135394152727688
27	22.267096977854415	26.62602029040453	28.770305672699642	22.336577059041414
28	22.12643153951521	26.067720870912197	29.188415544819808	22.617432044752785
29	21.19761134460613	26.076683484124676	29.919146124700884	22.80655904656831
30	20.9645834010817	26.2419762800079	30.8624809941045	21.930959324805897
31	21.92957130948538	25.71976525884899	30.23541532987572	22.115248101789906
32	22.44135238695012	25.819345443700907	28.997067718491458	22.742234450857516
33	23.161058159428233	25.715521897726273	28.95439616178072	22.169023781064777
34	22.196466826830417	27.321415965983313	28.84351356646174	21.638603640724526
35	21.474064338872438	27.79119966560728	29.099701537048507	21.63503445847177
36	21.837407092203083	25.523618865269714	30.317109945883264	22.321864096643935
37	21.98299007053497	25.508985218033413	30.023643849545483	22.48438086188613
38	21.88138734907316	25.2500608743862	30.739978727673773	22.128573048866865
39	22.683184313364684	24.79808342844543	30.621283588979313	21.89744866921057
40	21.647645569098177	25.337664469901082	30.030227007922793	22.984462953077944
41	21.899471205820465	25.20227348977985	30.117830603437678	22.780424700962012
42	20.603778732908573	26.336995843092403	30.155544962575142	22.903680461423882
43	20.933134939676854	25.911549318563793	29.936198884352944	23.219116857406412
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	180.0
1	249.0
2	318.0
3	620.5
4	923.0
5	923.0
6	1784.5
7	2646.0
8	2942.0
9	3238.0
10	5363.0
11	7488.0
12	7488.0
13	14438.5
14	21389.0
15	32514.5
16	43640.0
17	41760.5
18	39881.0
19	39881.0
20	45584.5
21	51288.0
22	43958.0
23	36628.0
24	40895.5
25	45163.0
26	45163.0
27	51178.5
28	57194.0
29	62379.0
30	67564.0
31	73937.5
32	80311.0
33	80311.0
34	89206.0
35	98101.0
36	106989.0
37	115877.0
38	122323.0
39	128769.0
40	128769.0
41	135401.5
42	142034.0
43	155131.0
44	168228.0
45	197661.5
46	227095.0
47	227095.0
48	228399.5
49	229704.0
50	212833.5
51	195963.0
52	186884.0
53	177805.0
54	177805.0
55	160745.0
56	143685.0
57	127336.5
58	110988.0
59	101828.5
60	92669.0
61	92669.0
62	82880.5
63	73092.0
64	63916.5
65	54741.0
66	45905.0
67	37069.0
68	37069.0
69	31361.0
70	25653.0
71	21782.5
72	17912.0
73	14313.5
74	10715.0
75	10715.0
76	8183.0
77	5651.0
78	4404.0
79	3157.0
80	2550.0
81	1943.0
82	1943.0
83	1529.0
84	1115.0
85	860.0
86	605.0
87	532.5
88	460.0
89	460.0
90	465.5
91	471.0
92	293.0
93	115.0
94	93.0
95	71.0
96	71.0
97	48.5
98	26.0
99	23.5
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2521586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.78594538710807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64138517341735	38.65449762023178
2	12.498937594456237	12.445428489489403
3	4.195725724811461	6.266645153842434
4	1.85353737380351	3.6912044186058104
5	1.0020953709537037	2.4945132705487447
6	0.5872524618698719	1.7542151397058927
7	0.390130299050387	1.3596104033665093
8	0.25380363487480895	1.0108683123941404
9	0.18960722558942247	0.849579748037646
>10	1.182648199695152	11.236790083047099
>50	0.12059222442538059	4.133118463073526
>100	0.07430587110140473	6.812536068848477
>500	0.005827646035582235	1.9630604608011242
>1k	0.0034327230072601135	3.2948987350443626
>5k	3.9915383805350156E-4	1.2977772357169137
>10k+	3.193230704428012E-4	2.735256397246002
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21649	0.8585469621103544	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20028	0.7942620239801459	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15553	0.6167943508569607	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11591	0.4596710165744892	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8728	0.3461313633562369	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6882	0.2729234695941364	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6448	0.25571207961973136	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5578	0.22120998450974902	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5017	0.1989620818008983	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4969	0.19705851793276136	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4695	0.18619234085214623	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3967	0.15732162218540235	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3605	0.14296557801320278	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3286	0.13031480980620927	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3171	0.12575418803879782	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2715	0.1076703312914967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.965758058618663E-5	0.0
5	0.0	0.0	0.0	3.965758058618663E-5	0.0
6	0.0	0.0	0.0	3.965758058618663E-5	0.0
7	0.0	0.0	0.0	7.931516117237325E-5	0.0
8	3.965758058618663E-5	0.0	0.0	7.931516117237325E-5	0.0
9	3.965758058618663E-5	0.0	0.0	1.1897274175855989E-4	0.0
10	3.965758058618663E-5	0.0	0.0	1.1897274175855989E-4	0.0
11	3.965758058618663E-5	0.0	0.0	2.3794548351711977E-4	0.0
12	3.965758058618663E-5	0.0	0.0	5.552061282066128E-4	0.0
13	3.965758058618663E-5	0.0	0.0	6.741788699651727E-4	0.0
14	7.931516117237325E-5	0.0	0.0	8.328091923099192E-4	0.0
15	7.931516117237325E-5	0.0	0.0	0.001189727417585599	0.0
16	7.931516117237325E-5	0.0	0.0	0.001824248706964585	0.0
17	7.931516117237325E-5	0.0	0.0	0.0028553458022054375	0.0
18	7.931516117237325E-5	0.0	0.0	0.0033312367692396767	0.0
19	7.931516117237325E-5	0.0	0.0	0.0039261004780324765	0.0
20	7.931516117237325E-5	0.0	0.0	0.0052744582179628215	0.0
21	7.931516117237325E-5	0.0	0.0	0.0074952827307892735	0.0
22	7.931516117237325E-5	0.0	0.0	0.013642207721648201	0.0
23	7.931516117237325E-5	0.0	0.0	0.021732354161230275	0.0
24	7.931516117237325E-5	0.0	0.0	0.03450209510998237	3.965758058618663E-5
25	7.931516117237325E-5	0.0	0.0	0.039459292683255695	3.965758058618663E-5
26	7.931516117237325E-5	0.0	0.0	0.046359711705252174	3.965758058618663E-5
27	7.931516117237325E-5	0.0	0.0	0.08316194648923336	3.965758058618663E-5
28	7.931516117237325E-5	0.0	0.0	0.15113503961395724	3.965758058618663E-5
29	7.931516117237325E-5	0.0	0.0	0.23731096222774078	3.965758058618663E-5
30	7.931516117237325E-5	0.0	0.0	0.38749421990762956	3.965758058618663E-5
31	7.931516117237325E-5	0.0	0.0	0.6087438619979648	3.965758058618663E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5180	0.0	30.357143	1
GTATCAA	8165	0.0	19.236374	2
TAGGACG	155	2.1827873E-11	17.903225	4
TATATCG	125	8.592906E-9	17.76	5
CGAACGA	200	0.0	17.575	16
GCGCGAC	95	3.6098427E-6	17.526316	25
GTATTGG	835	0.0	17.05988	1
ATTGGAC	825	0.0	16.818182	3
CGTAATA	70	0.0025936444	15.857143	2
ACGGACC	320	0.0	15.609376	8
CGAATTA	180	2.0190782E-10	15.416666	15
ACGAACG	230	0.0	15.282609	15
GTATTAG	440	0.0	15.136364	1
TGGACCC	1235	0.0	15.129556	5
AAGACGG	380	0.0	15.092106	5
GTTACGA	75	0.0041062133	14.8	25
ACGTAGA	75	0.0041062133	14.8	7
TTGGACC	1325	0.0	14.6603775	4
GGACCCT	1285	0.0	14.252918	6
TAGACTG	340	0.0	14.147059	5
>>END_MODULE