FastQCFastQC Report
Wed 25 May 2016
SRR2088115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3271512
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT370371.1321064999914412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT360711.1025788687310332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT294760.9009901232213118No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG198830.6077617933236986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143570.4388490703992527No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA105330.3219612216002876No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT105090.32122761585468734No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT98410.3008089226021485No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA88580.27076165393860696No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA85420.2611025116215377No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG79970.2444435478151998No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT73180.22368861859592748No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT70970.21693333235519233No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58320.1782661961808485No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA57180.17478156888924753No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT50010.15286509723944158No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA48780.14910536779324055No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43480.13290490757790283No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC40090.12254272642130001No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38370.1172852185778319No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37650.1150844013410313No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG37100.11340322150736418No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT35790.10939895681262976No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.10777891079109599No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34540.10557809355429537No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG33020.10093192383216079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87550.027.9560241
GTATTGG12850.021.4513631
ATTGGAC12150.020.860083
CAATGCG2100.017.61904719
TATACCG1705.456968E-1217.4117645
TGCGACG2150.017.20930322
TAATACG650.001580776817.0769234
GTATCAA144000.017.0097222
TAGGTCG2550.016.68627421
TTGGACC20150.016.5260544
TATTGGA16850.016.4688422
GGACCCT20600.016.3446626
CTGTGCG3600.015.9305559
CGACTAT700.002593865615.85714336
TGGACCC21650.015.7228645
CATTGCG2850.015.57894729
CGACGAG2750.015.47272724
CGTCTGT8150.015.43558234
TGTGCGC4050.015.07407610
CGATCTG3350.014.91044833
Produced by FastQC (version 0.11.5)