##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088115_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3271512
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.611079830977236	31.0	31.0	34.0	30.0	34.0
2	31.791689897515276	31.0	31.0	34.0	30.0	34.0
3	31.88658088370148	31.0	31.0	34.0	30.0	34.0
4	35.59078279401084	37.0	35.0	37.0	33.0	37.0
5	35.48376836154048	37.0	35.0	37.0	33.0	37.0
6	35.49669663446137	37.0	35.0	37.0	33.0	37.0
7	35.36597787200536	37.0	35.0	37.0	33.0	37.0
8	35.410283685341824	37.0	35.0	37.0	33.0	37.0
9	36.922520840516555	39.0	37.0	39.0	33.0	39.0
10	36.78614139272605	39.0	37.0	39.0	32.0	39.0
11	36.85209774562954	39.0	37.0	39.0	32.0	39.0
12	36.74128048437542	39.0	35.0	39.0	32.0	39.0
13	36.75063701432243	39.0	35.0	39.0	32.0	39.0
14	37.7579950799508	40.0	37.0	41.0	32.0	41.0
15	37.760798982244296	40.0	37.0	41.0	32.0	41.0
16	37.64700328166303	39.0	36.0	41.0	32.0	41.0
17	37.66444842629341	39.0	37.0	41.0	32.0	41.0
18	37.643974712609946	39.0	37.0	41.0	32.0	41.0
19	37.684459357019016	39.0	37.0	41.0	32.0	41.0
20	37.594679768865284	39.0	37.0	41.0	32.0	41.0
21	37.509367534033196	39.0	36.0	41.0	32.0	41.0
22	37.40897817278372	39.0	36.0	41.0	32.0	41.0
23	37.18729718857825	39.0	36.0	41.0	31.0	41.0
24	37.029522740555436	39.0	36.0	41.0	31.0	41.0
25	36.925216535962576	39.0	36.0	40.0	31.0	41.0
26	37.056135817322385	39.0	36.0	41.0	31.0	41.0
27	36.96893638170974	39.0	36.0	41.0	31.0	41.0
28	36.87077137421473	39.0	35.0	41.0	30.0	41.0
29	36.78942091607795	39.0	35.0	41.0	30.0	41.0
30	36.59906061784276	39.0	35.0	40.0	30.0	41.0
31	36.420886733718234	39.0	35.0	40.0	30.0	41.0
32	36.25930823423543	38.0	35.0	40.0	30.0	41.0
33	36.05059098056189	38.0	35.0	40.0	29.0	41.0
34	35.90627514128024	38.0	35.0	40.0	29.0	41.0
35	35.69903121247912	38.0	35.0	40.0	27.0	41.0
36	35.61165448881129	38.0	35.0	40.0	27.0	41.0
37	35.461869924365246	38.0	34.0	40.0	26.0	41.0
38	35.33490691765765	38.0	34.0	40.0	25.0	41.0
39	35.224031579282	38.0	34.0	40.0	25.0	41.0
40	35.02370402431659	38.0	34.0	40.0	24.0	41.0
41	34.867680143004215	38.0	34.0	40.0	24.0	41.0
42	34.653264912370794	38.0	33.0	40.0	23.0	41.0
43	34.33921226637714	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	9.0
14	20.0
15	26.0
16	70.0
17	118.0
18	266.0
19	558.0
20	1200.0
21	2490.0
22	4579.0
23	7875.0
24	12721.0
25	19515.0
26	28616.0
27	39811.0
28	54459.0
29	70859.0
30	89773.0
31	110705.0
32	136251.0
33	169083.0
34	207364.0
35	254856.0
36	327098.0
37	450360.0
38	598760.0
39	684062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.64961400111019	22.613305407407953	13.820276373737892	22.916804217743966
2	18.125044322013796	22.726280692230382	35.85317736875182	23.295497617004003
3	19.655773844020747	25.529204844732345	32.070614443718995	22.744406867527918
4	13.381580137868973	15.746633360965816	35.156190776619496	35.715595724545715
5	11.520819731060133	40.513041064804284	34.280082114936455	13.686057089199121
6	32.007952286282304	36.52998980288014	15.258265902738549	16.203792008099008
7	25.4976903645776	34.8049770259134	21.000350908081643	18.69698170142735
8	28.06350702672037	34.09487723107848	19.702510643396693	18.139105098804468
9	25.627538581548837	13.920474691824452	22.72197075847498	37.73001596815173
10	16.663029204844733	28.057577046943432	32.848389368585536	22.4310043796263
11	32.47266095921397	21.903113911854824	24.101699764512556	21.522525364418655
12	20.080531570723263	27.463723195880068	31.2576570099697	21.198088223426968
13	31.01590334988837	20.973941101240037	27.899240473518056	20.110915075353535
14	20.867170898349144	22.45897309867731	27.769392256546823	28.904463746426728
15	23.43793939927471	29.069463905374644	25.757019995647273	21.73557669970338
16	22.378093065224885	28.45213467045207	26.06702955697549	23.102742707347552
17	20.047794414325853	27.23318759032521	27.359153810225973	25.359864185122966
18	21.135212097647816	26.589662516903502	30.9923056984049	21.282819687043787
19	20.687406923771025	24.962891776035057	31.94000205409609	22.409699246097826
20	24.79058612653721	24.10912752268676	30.79713600316918	20.303150347606856
21	23.957118298817186	25.09607178576756	29.64354096821286	21.303268947202394
22	22.56100543112787	24.54229114855761	30.73743883562096	22.15926458469356
23	21.487526256972313	26.270513450661348	31.6184076353686	20.62355265699774
24	20.470198489261236	25.574413298804956	30.176627810015678	23.778760401918134
25	21.51521987386872	26.4681284983824	30.993895177520365	21.02275645022852
26	22.863403832845485	25.55689846162875	29.81181178610991	21.767885919415857
27	21.55648519705873	26.827595313726498	29.5198672662671	22.096052222947677
28	21.346796221441338	26.257277980334475	29.75819743286896	22.637728365355226
29	20.420374432372554	26.093103127850366	30.909683351306672	22.576839088470408
30	19.917426559951483	26.404977270448647	32.354825536326935	21.322770633272935
31	21.221196804413374	25.860061035998033	31.359475374077796	21.559266785510797
32	22.236109786545182	25.92538251426252	29.61422730529492	22.224280393897377
33	22.980352815456584	25.80449040076882	29.41211892238207	21.80303786139253
34	21.43009104047303	28.107706772892776	29.496177914065424	20.96602427256877
35	20.394239727685548	28.675364785456996	29.78084751026437	21.149547976593087
36	20.971067812069773	25.550632245885087	31.676393056177083	21.801906885868064
37	21.167826986420955	25.777316421275543	31.014313870772902	22.040542721530596
38	21.21175163043877	25.26807176620474	31.868536627712203	21.651639975644287
39	22.147251790609356	24.83747576044349	31.748255852339835	21.26701659660732
40	20.765444234959247	25.40608134709578	31.000436495418633	22.82803792252634
41	21.34337272796187	25.121381183990763	30.98457227117003	22.550673816877335
42	19.339100697169993	26.5905795240855	31.357396824465262	22.712922954279243
43	19.89492931708641	26.3348567879317	30.857383375026593	22.9128305199553
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	243.0
1	384.5
2	526.0
3	1037.5
4	1549.0
5	1549.0
6	2817.0
7	4085.0
8	4643.5
9	5202.0
10	8448.0
11	11694.0
12	11694.0
13	22726.5
14	33759.0
15	53557.5
16	73356.0
17	69382.5
18	65409.0
19	65409.0
20	71926.5
21	78444.0
22	63885.0
23	49326.0
24	55721.0
25	62116.0
26	62116.0
27	67786.5
28	73457.0
29	78054.0
30	82651.0
31	89703.0
32	96755.0
33	96755.0
34	106898.0
35	117041.0
36	129239.0
37	141437.0
38	149418.5
39	157400.0
40	157400.0
41	165684.0
42	173968.0
43	195737.5
44	217507.0
45	268682.5
46	319858.0
47	319858.0
48	323884.0
49	327910.0
50	299100.0
51	270290.0
52	253248.0
53	236206.0
54	236206.0
55	209458.0
56	182710.0
57	155914.5
58	129119.0
59	115221.5
60	101324.0
61	101324.0
62	90097.0
63	78870.0
64	68802.0
65	58734.0
66	50413.5
67	42093.0
68	42093.0
69	35828.0
70	29563.0
71	24714.0
72	19865.0
73	16066.0
74	12267.0
75	12267.0
76	9514.5
77	6762.0
78	5393.5
79	4025.0
80	3258.5
81	2492.0
82	2492.0
83	1944.5
84	1397.0
85	1086.0
86	775.0
87	651.5
88	528.0
89	528.0
90	517.0
91	506.0
92	322.0
93	138.0
94	120.5
95	103.0
96	103.0
97	66.0
98	29.0
99	26.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3271512.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.20506271146135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17262767817122	31.3046040263109
2	13.416375925643436	11.324779746046431
3	5.03476889334035	6.37478210643433
4	2.414408962453119	4.076011266857928
5	1.3076380013237592	2.759447192487962
6	0.8060749572186048	2.041226647172984
7	0.5097204981738582	1.5058949913521504
8	0.35154649721680853	1.1869633568823983
9	0.27366547743882286	1.039506177354078
>10	1.4424438243625892	11.485332878678499
>50	0.1505035818002033	4.4347149194887905
>100	0.10510181186409087	8.481018912548064
>500	0.008361766654878033	2.399350796904809
>1k	0.005598748107943874	4.71016749493858
>5k	6.543991294999334E-4	2.031650425344907
>10k+	5.089771007221705E-4	4.8445490611971955
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37037	1.1321064999914412	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36071	1.1025788687310332	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29476	0.9009901232213118	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	19883	0.6077617933236986	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14357	0.4388490703992527	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10533	0.3219612216002876	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10509	0.32122761585468734	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9841	0.3008089226021485	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8858	0.27076165393860696	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8542	0.2611025116215377	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7997	0.2444435478151998	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7318	0.22368861859592748	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7097	0.21693333235519233	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5832	0.1782661961808485	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5718	0.17478156888924753	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5001	0.15286509723944158	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4878	0.14910536779324055	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4348	0.13290490757790283	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4009	0.12254272642130001	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3837	0.1172852185778319	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3765	0.1150844013410313	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3710	0.11340322150736418	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3579	0.10939895681262976	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3526	0.10777891079109599	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3454	0.10557809355429537	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3302	0.10093192383216079	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.056690606667498E-5	0.0
3	0.0	0.0	0.0	3.056690606667498E-5	0.0
4	0.0	0.0	0.0	6.113381213334996E-5	0.0
5	0.0	0.0	0.0	6.113381213334996E-5	0.0
6	0.0	0.0	0.0	6.113381213334996E-5	0.0
7	0.0	0.0	0.0	6.113381213334996E-5	0.0
8	0.0	0.0	0.0	9.170071820002494E-5	0.0
9	0.0	0.0	0.0	3.056690606667498E-4	0.0
10	0.0	0.0	0.0	3.9736977886677474E-4	0.0
11	0.0	0.0	0.0	7.336057456001995E-4	0.0
12	3.056690606667498E-5	0.0	0.0	9.781409941335994E-4	0.0
13	3.056690606667498E-5	0.0	0.0	0.0010392748062669494	0.0
14	3.056690606667498E-5	0.0	0.0	0.0011921093366003242	0.0
15	3.056690606667498E-5	0.0	0.0	0.001742313645800474	0.0
16	3.056690606667498E-5	0.0	0.0	0.003026123700600823	0.0
17	3.056690606667498E-5	0.0	0.0	0.005043539501001372	0.0
18	3.056690606667498E-5	0.0	0.0	0.005777145246601572	0.0
19	3.056690606667498E-5	0.0	0.0	0.006602451710401796	0.0
20	3.056690606667498E-5	0.0	0.0	0.00840589916833562	0.0
21	3.056690606667498E-5	0.0	0.0	0.011645991211403167	0.0
22	3.056690606667498E-5	0.0	0.0	0.020418693252538887	0.0
23	3.056690606667498E-5	0.0	0.0	0.034020966452209256	0.0
24	3.056690606667498E-5	0.0	0.0	0.05138296909808064	0.0
25	3.056690606667498E-5	0.0	0.0	0.05749635031141564	0.0
26	3.056690606667498E-5	0.0	0.0	0.06633018616468471	0.0
27	3.056690606667498E-5	0.0	0.0	0.11636821139583166	0.0
28	3.056690606667498E-5	0.0	0.0	0.19874602324552074	0.0
29	3.056690606667498E-5	0.0	0.0	0.30145082762954867	0.0
30	3.056690606667498E-5	0.0	0.0	0.492616258170534	0.0
31	3.056690606667498E-5	0.0	0.0	0.7408501023380015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8755	0.0	27.956024	1
GTATTGG	1285	0.0	21.451363	1
ATTGGAC	1215	0.0	20.86008	3
CAATGCG	210	0.0	17.619047	19
TATACCG	170	5.456968E-12	17.411764	5
TGCGACG	215	0.0	17.209303	22
TAATACG	65	0.0015807768	17.076923	4
GTATCAA	14400	0.0	17.009722	2
TAGGTCG	255	0.0	16.686274	21
TTGGACC	2015	0.0	16.526054	4
TATTGGA	1685	0.0	16.468842	2
GGACCCT	2060	0.0	16.344662	6
CTGTGCG	360	0.0	15.930555	9
CGACTAT	70	0.0025938656	15.857143	36
TGGACCC	2165	0.0	15.722864	5
CATTGCG	285	0.0	15.578947	29
CGACGAG	275	0.0	15.472727	24
CGTCTGT	815	0.0	15.435582	34
TGTGCGC	405	0.0	15.074076	10
CGATCTG	335	0.0	14.910448	33
>>END_MODULE