FastQCFastQC Report
Wed 25 May 2016
SRR2088114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497804
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT203920.8163971232330478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT196450.7864908535657722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT153230.6134588622646132No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78260.3133152160858098No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG44830.179477653170545No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA41250.16514506342371138No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG38830.155456553036187No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG36120.14460702280883528No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA30090.12046581717380547No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA26030.10421153941622321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.027.2078151
TAGACCG500.00703713818.4999985
GTATCAA87700.017.4874572
TATACCG1603.45608E-1117.343755
ACGTTTA1708.54925E-1116.3235326
CTAGTAC3100.015.51612953
CTAGGCA4200.014.976194
GTACTAG2600.014.9423071
CGGTATA2151.2732926E-1114.62790726
TAATACT7700.014.4155844
TTAACGG3750.014.30666635
CGACGAG2600.014.23076924
CAATGCG2353.6379788E-1214.17021419
CGACACA800.006301348613.8750014
GTATTAG6000.013.8751
ATGCGAC2405.456968E-1213.87499921
TAAACGT1356.573884E-613.7037044
TCACGTT1907.1377144E-913.63157924
CTAATAC6800.013.60294153
GTCTAAC1501.3071385E-613.5666671
Produced by FastQC (version 0.11.5)