FastQCFastQC Report
Wed 25 May 2016
SRR2088113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797283
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT165520.9209456718836155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT157820.8781032258136309No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT108800.6053581990148463No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61270.34090346372830543No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG39860.2217792078376082No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG39070.2173836841499085No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA36550.20336251998154994No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34650.19279100731493037No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA32790.18244205280971332No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG28790.1601862366694616No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.11061140621705097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37300.029.0147441
TATATCG705.1045645E-621.1428595
GTATCAA60850.017.7855382
TTACTCG650.001580402617.07692319
CGAATTA1005.881502E-616.6515
TATACTG2700.016.4444455
TAGCGCA803.3838933E-416.18754
TTTGGAC5350.015.9065423
TCTATAC2350.015.7446813
ACGTTTA1201.93688E-615.41666626
TTGGACC8300.015.37951854
GTATACT1752.240995E-914.84
CGTTTAT1405.9997546E-714.5357151
ATAACAC3100.014.322583
ATTGGAC6250.014.2083
AATGCGA1702.438901E-814.14705920
TGGACCC8000.014.1062495
CATCCGT1051.6564499E-414.09523911
GTATTAG4350.014.0344831
TAATACG800.006300444213.8754
Produced by FastQC (version 0.11.5)