##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088113_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1797283
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.110990867882244	31.0	31.0	33.0	30.0	34.0
2	31.330058204523162	31.0	31.0	34.0	30.0	34.0
3	31.381587652028088	31.0	31.0	34.0	28.0	34.0
4	35.13873496828268	35.0	35.0	37.0	32.0	37.0
5	34.973327517146714	35.0	35.0	37.0	32.0	37.0
6	35.046674897609336	36.0	35.0	37.0	32.0	37.0
7	34.92210798188154	36.0	35.0	37.0	32.0	37.0
8	34.957254366730226	36.0	35.0	37.0	32.0	37.0
9	36.44489821580686	38.0	35.0	39.0	32.0	39.0
10	36.1782034326258	38.0	35.0	39.0	32.0	39.0
11	36.29490681211584	38.0	35.0	39.0	32.0	39.0
12	36.12795480733975	38.0	35.0	39.0	31.0	39.0
13	36.188827802855755	38.0	35.0	39.0	32.0	39.0
14	37.08711148995456	39.0	36.0	40.0	31.0	41.0
15	37.11201074065687	39.0	36.0	40.0	31.0	41.0
16	36.951704878975654	38.0	36.0	40.0	31.0	41.0
17	36.93524336456752	38.0	36.0	40.0	31.0	41.0
18	36.90952509983124	38.0	36.0	40.0	31.0	41.0
19	36.937812242145505	39.0	36.0	40.0	31.0	41.0
20	36.85215795175273	39.0	36.0	40.0	31.0	41.0
21	36.75745667209894	39.0	36.0	40.0	30.0	41.0
22	36.62788943087983	38.0	35.0	40.0	30.0	41.0
23	36.418184559693714	38.0	35.0	40.0	30.0	41.0
24	36.27456555255906	38.0	35.0	40.0	29.0	41.0
25	36.117410001652495	38.0	34.0	40.0	29.0	41.0
26	36.25944105630555	38.0	35.0	40.0	30.0	41.0
27	36.16312344800457	38.0	35.0	40.0	29.0	41.0
28	36.08574164447113	38.0	35.0	40.0	29.0	41.0
29	36.00097424835154	38.0	35.0	40.0	29.0	41.0
30	35.77780627758678	38.0	34.0	40.0	27.0	41.0
31	35.606942813124036	38.0	34.0	40.0	27.0	41.0
32	35.4459681641678	38.0	34.0	40.0	27.0	41.0
33	35.28279853534474	38.0	34.0	40.0	26.0	41.0
34	35.160137830269356	38.0	34.0	40.0	26.0	41.0
35	34.949680156102296	38.0	33.0	40.0	25.0	41.0
36	34.88062647896853	38.0	33.0	40.0	25.0	41.0
37	34.71377295617886	38.0	33.0	40.0	25.0	41.0
38	34.62990580782214	38.0	33.0	40.0	24.0	41.0
39	34.54426375812824	38.0	33.0	40.0	24.0	41.0
40	34.387285140959996	38.0	33.0	40.0	23.0	41.0
41	34.250405751348005	38.0	33.0	40.0	23.0	41.0
42	34.06724038451374	37.0	33.0	40.0	22.0	41.0
43	33.73987902851137	37.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	5.0
14	6.0
15	22.0
16	42.0
17	104.0
18	270.0
19	561.0
20	1061.0
21	2189.0
22	3806.0
23	6263.0
24	9930.0
25	14846.0
26	21082.0
27	28780.0
28	38164.0
29	48822.0
30	61256.0
31	74722.0
32	90326.0
33	108529.0
34	130034.0
35	158785.0
36	200698.0
37	259841.0
38	334838.0
39	202299.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.32968987076604	24.127808475348623	15.0005869971507	22.541914656734637
2	20.330298567337476	24.72259516169685	33.3967438628196	21.55036240814607
3	20.60048417528013	25.63953478667522	32.1854710693864	21.574509968658248
4	15.274166617054744	18.195965799487336	36.00234353743957	30.52752404601835
5	12.298730917724141	39.52944527934666	35.39398080324579	12.777842999683411
6	31.850687955096664	39.13785419435893	13.752870304787837	15.258587545756566
7	25.595468270717518	33.494335616594604	22.151380723013574	18.758815389674304
8	26.35767433398079	35.96790266196253	19.22195892355294	18.452464080503738
9	24.723262836181057	14.485587411665273	21.623361485086097	39.167788267067564
10	15.772418700894628	28.56528437647271	34.479044201720036	21.18325272091262
11	33.77030773673372	22.868852595834934	23.235016410882427	20.125823256548912
12	20.195650879688955	27.66525917176093	31.21867841625387	20.92041153229625
13	30.47171758704667	21.753669288587275	26.35934352019131	21.415269604174746
14	20.24572646600452	22.835246313463156	27.784939823055133	29.13408739747719
15	22.715732580790004	30.264404659700222	25.105005722526723	21.914857036983047
16	22.02496768733694	28.79424108501555	26.43723887668219	22.743552350965317
17	21.577737061998583	27.653463589206595	27.46061694235132	23.308182406443503
18	22.32825882178822	26.4951596381872	29.435876264339004	21.740705275685578
19	22.526168666815412	25.616722575131462	30.27903785881244	21.578070899240686
20	23.084900931016428	25.599307399001713	30.286549196759776	21.02924247322208
21	22.42807615717725	26.077195411073266	30.496087705720242	20.998640726029233
22	21.379382100648588	26.492043823927563	30.12580656468681	22.00276751073704
23	22.078771122856	26.573611390081588	30.732667031291122	20.614950455771293
24	22.148153629673235	26.67498663260043	28.75991148861921	22.416948249107126
25	22.16022740992932	26.63503744262868	29.777836879333975	21.426898268108026
26	21.97088605411613	27.27550419160477	29.597564768597934	21.156044985681163
27	21.433686292030803	27.192601276482335	29.15072362004203	22.22298881144483
28	21.55442409459167	26.967094219441236	29.78712868257253	21.691353003394568
29	22.041325712200027	26.514577837769565	29.248537931978436	22.195558518051968
30	22.000263731421263	26.76656931601757	30.034446439431072	21.198720513130098
31	22.301384923798867	26.6855025057267	30.178942325721657	20.834170244752773
32	21.63387735821237	26.589524298621864	29.65759983263626	22.118998510529504
33	21.79406359488183	26.553748074176408	29.961614281112105	21.690574049829657
34	21.153596845905735	26.96909724289386	30.48829817007116	21.389007741129248
35	21.0266274148256	28.227830564246144	29.555668194713906	21.18987382621435
36	22.70738664973741	26.540283305411556	29.25165374623807	21.500676298612962
37	21.63944131224743	27.072086032082872	29.56023063702266	21.728242018647034
38	21.681393525671805	25.896366904933725	30.511722416558772	21.910517152835695
39	21.19699568737923	26.08236988832588	31.068674215468572	21.651960208826324
40	20.526650505234848	26.55597365579043	30.535035383965685	22.382340455009032
41	21.494778507335795	25.43255569657088	30.918614375142923	22.154051420950402
42	20.57655917292936	27.17807935645082	29.97580236390151	22.269559106718305
43	21.050886254418476	26.70152669334768	29.570746510148933	22.676840542084914
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	207.0
2	262.0
3	392.5
4	523.0
5	523.0
6	1086.5
7	1650.0
8	1879.0
9	2108.0
10	3412.5
11	4717.0
12	4717.0
13	9825.0
14	14933.0
15	24122.5
16	33312.0
17	31311.5
18	29311.0
19	29311.0
20	33815.0
21	38319.0
22	32750.5
23	27182.0
24	31384.5
25	35587.0
26	35587.0
27	40749.0
28	45911.0
29	49158.0
30	52405.0
31	57894.5
32	63384.0
33	63384.0
34	72477.0
35	81570.0
36	88312.0
37	95054.0
38	101363.5
39	107673.0
40	107673.0
41	110430.0
42	113187.0
43	117804.5
44	122422.0
45	124819.5
46	127217.0
47	127217.0
48	148421.5
49	169626.0
50	156035.0
51	142444.0
52	130768.5
53	119093.0
54	119093.0
55	106883.5
56	94674.0
57	83218.5
58	71763.0
59	65536.0
60	59309.0
61	59309.0
62	52706.5
63	46104.0
64	39846.0
65	33588.0
66	28140.5
67	22693.0
68	22693.0
69	19193.0
70	15693.0
71	13000.0
72	10307.0
73	8421.0
74	6535.0
75	6535.0
76	5156.0
77	3777.0
78	2971.0
79	2165.0
80	1702.5
81	1240.0
82	1240.0
83	952.0
84	664.0
85	501.0
86	338.0
87	265.0
88	192.0
89	192.0
90	156.0
91	120.0
92	85.5
93	51.0
94	34.0
95	17.0
96	17.0
97	11.5
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1797283.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.63800196653282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79708432412062	39.90126910961872
2	12.0856278677833	12.239840954711893
3	3.8656051060647565	5.872395568882393
4	1.7067861751682025	3.457129667784739
5	0.8672385980473833	2.195761491668828
6	0.5284894229207527	1.605698906229172
7	0.33815932392709247	1.1986598757015063
8	0.24572361630386128	0.9954362372494786
9	0.19444155609561467	0.8861518709950892
>10	1.1460005785171676	11.225456309051228
>50	0.1285057541922409	4.521342318959411
>100	0.08699023439343748	8.41051339956753
>500	0.006268284947537671	2.188816158370331
>1k	0.0026392778726474406	2.553897284261439
>5k	1.0996991136031003E-4	0.34119158912977704
>10k+	3.299097340809301E-4	2.4064392578185387
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16552	0.9209456718836155	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15782	0.8781032258136309	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10880	0.6053581990148463	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6127	0.34090346372830543	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3986	0.2217792078376082	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3907	0.2173836841499085	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3655	0.20336251998154994	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3465	0.19279100731493037	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3279	0.18244205280971332	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2879	0.1601862366694616	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1988	0.11061140621705097	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	5.563954035062925E-5	0.0	0.0	2.7819770175314625E-4	0.0
10	5.563954035062925E-5	0.0	0.0	3.894767824544048E-4	0.0
11	1.112790807012585E-4	0.0	0.0	5.007558631556633E-4	0.0
12	1.112790807012585E-4	0.0	0.0	6.67674484207551E-4	0.0
13	1.112790807012585E-4	0.0	0.0	6.67674484207551E-4	0.0
14	1.112790807012585E-4	0.0	0.0	9.458721859606973E-4	0.0
15	1.112790807012585E-4	0.0	0.0	0.001112790807012585	0.0
16	1.112790807012585E-4	0.0	0.0	0.0015579071298176192	0.0
17	1.112790807012585E-4	0.0	0.0	0.0018361048315707655	0.0
18	1.112790807012585E-4	0.0	0.0	0.001947383912272024	0.0
19	2.7819770175314625E-4	0.0	0.0	0.0022812211543757994	0.0
20	2.7819770175314625E-4	0.0	0.0	0.0032270933403364967	0.0
21	2.7819770175314625E-4	0.0	0.0	0.004562442308751599	0.0
22	2.7819770175314625E-4	0.0	0.0	0.007233140245581803	0.0
23	2.7819770175314625E-4	0.0	0.0	0.009013605536801939	0.0
24	2.7819770175314625E-4	0.0	0.0	0.011239187150827109	0.0
25	2.7819770175314625E-4	0.0	0.0	0.013186571063099133	0.0
26	2.7819770175314625E-4	0.0	0.0	0.017526455210448214	0.0
27	2.7819770175314625E-4	0.0	0.0	0.04851767918574871	0.0
28	2.7819770175314625E-4	0.0	0.0	0.11061140621705096	0.0
29	3.894767824544048E-4	0.0	0.0	0.18700449511846493	0.0
30	3.894767824544048E-4	0.0	0.0	0.30156630870041057	0.0
31	3.894767824544048E-4	0.0	0.0	0.46792853434879206	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3730	0.0	29.014744	1
TATATCG	70	5.1045645E-6	21.142859	5
GTATCAA	6085	0.0	17.785538	2
TTACTCG	65	0.0015804026	17.076923	19
CGAATTA	100	5.881502E-6	16.65	15
TATACTG	270	0.0	16.444445	5
TAGCGCA	80	3.3838933E-4	16.1875	4
TTTGGAC	535	0.0	15.906542	3
TCTATAC	235	0.0	15.744681	3
ACGTTTA	120	1.93688E-6	15.416666	26
TTGGACC	830	0.0	15.3795185	4
GTATACT	175	2.240995E-9	14.8	4
CGTTTAT	140	5.9997546E-7	14.535715	1
ATAACAC	310	0.0	14.32258	3
ATTGGAC	625	0.0	14.208	3
AATGCGA	170	2.438901E-8	14.147059	20
TGGACCC	800	0.0	14.106249	5
CATCCGT	105	1.6564499E-4	14.095239	11
GTATTAG	435	0.0	14.034483	1
TAATACG	80	0.0063004442	13.875	4
>>END_MODULE