FastQCFastQC Report
Wed 25 May 2016
SRR2088112_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088112_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151630
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT297790.944876143455926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT282220.8954731361232124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT224060.7109337073197044No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111510.3538169137874687No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG78170.2480303842773422No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG75800.2405104660128251No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA72030.22854840193804477No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA67230.21331818773142786No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA65090.20652805056431117No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG62180.19729473320154967No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA36430.11559097990563613No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34790.11038732338504202No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.10769030628595361No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33210.10537404454203064No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC31820.10096362834469783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69800.029.4727761
GTATTGG10150.017.862071
GTATCAA119600.017.1542642
CAATGCG1403.479181E-815.85714219
CTAATAC8850.015.6779673
ATTGGAC13150.015.615973
GGGTAAG4050.015.0740741
TACTAGG3450.015.0144932
GTATTAG7950.014.8930831
TTGGACC17500.014.6942864
GCGACGA2650.014.66037723
GGACCCT16650.014.5555556
TGGACCC16800.014.5357155
GACCCTC16450.014.50767
CTCTAAT7700.014.41558551
TAATACT10950.014.191784
TATACCG1458.92338E-714.0344835
TAGTACT5200.013.8754
CGTACAC950.001245915713.63157923
CCTATCG950.001245915713.6315793
Produced by FastQC (version 0.11.5)