##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088112_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3151630
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49171508076773	31.0	31.0	34.0	30.0	34.0
2	31.703843408014265	31.0	31.0	34.0	30.0	34.0
3	31.779855820638844	31.0	31.0	34.0	30.0	34.0
4	35.48713840139864	37.0	35.0	37.0	33.0	37.0
5	35.36622097137037	37.0	35.0	37.0	33.0	37.0
6	35.40467631035369	37.0	35.0	37.0	33.0	37.0
7	35.285674079761904	37.0	35.0	37.0	32.0	37.0
8	35.311043491780445	37.0	35.0	37.0	32.0	37.0
9	36.85793287917681	39.0	37.0	39.0	33.0	39.0
10	36.664896260030524	39.0	35.0	39.0	32.0	39.0
11	36.726923845756005	39.0	35.0	39.0	32.0	39.0
12	36.593600771664185	38.0	35.0	39.0	32.0	39.0
13	36.62970780199452	38.0	35.0	39.0	32.0	39.0
14	37.626420614094926	39.0	36.0	41.0	32.0	41.0
15	37.621803320821286	39.0	36.0	41.0	32.0	41.0
16	37.50253138851959	39.0	36.0	41.0	32.0	41.0
17	37.48515276222145	39.0	36.0	41.0	32.0	41.0
18	37.45896536078156	39.0	36.0	41.0	32.0	41.0
19	37.49795217078147	39.0	36.0	41.0	32.0	41.0
20	37.40714423964742	39.0	36.0	41.0	32.0	41.0
21	37.31444807924788	39.0	36.0	41.0	31.0	41.0
22	37.19434736945644	39.0	36.0	40.0	31.0	41.0
23	36.98439093421499	39.0	36.0	40.0	31.0	41.0
24	36.84647626783601	39.0	36.0	40.0	30.0	41.0
25	36.68236785409455	39.0	35.0	40.0	30.0	41.0
26	36.87696271453184	39.0	36.0	40.0	31.0	41.0
27	36.80610985426589	39.0	36.0	40.0	30.0	41.0
28	36.73604896513867	39.0	35.0	40.0	30.0	41.0
29	36.658722946538774	39.0	35.0	40.0	30.0	41.0
30	36.43787373517831	39.0	35.0	40.0	30.0	41.0
31	36.25974273629836	38.0	35.0	40.0	30.0	41.0
32	36.107877193706116	38.0	35.0	40.0	29.0	41.0
33	35.95437472038279	38.0	35.0	40.0	29.0	41.0
34	35.83202089077715	38.0	35.0	40.0	28.0	41.0
35	35.64214581026326	38.0	34.0	40.0	27.0	41.0
36	35.55098758420246	38.0	34.0	40.0	27.0	41.0
37	35.391393025196486	38.0	34.0	40.0	26.0	41.0
38	35.31756646560669	38.0	34.0	40.0	26.0	41.0
39	35.23497872529453	38.0	34.0	40.0	25.0	41.0
40	35.0725294530132	38.0	34.0	40.0	25.0	41.0
41	34.932498421451754	38.0	34.0	40.0	24.0	41.0
42	34.754845270542546	38.0	33.0	40.0	24.0	41.0
43	34.42950473247177	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	7.0
14	9.0
15	27.0
16	49.0
17	125.0
18	252.0
19	607.0
20	1278.0
21	2632.0
22	4677.0
23	8092.0
24	12864.0
25	19701.0
26	28108.0
27	38996.0
28	53248.0
29	69979.0
30	88521.0
31	110213.0
32	135571.0
33	167589.0
34	205455.0
35	254767.0
36	327302.0
37	441669.0
38	613763.0
39	566124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.56942915253377	22.558580797872846	14.359141142837197	23.512848906756187
2	18.818516132921694	23.561300025700987	35.21076395389053	22.40941988748679
3	19.219292873846232	25.286470810342585	32.740772235319504	22.753464080491682
4	13.922319561623667	17.080780421559638	36.98184748844249	32.015052528374206
5	12.143652649581329	38.96015712504323	35.73344586769386	13.16274435768158
6	31.713335639018542	38.68138074583628	13.867966734673804	15.737316880471376
7	25.647744183168708	33.31177834961591	22.233352265335714	18.807125201879664
8	26.239691841999218	35.81251606311655	19.211614307517063	18.736177787367172
9	24.526736958335846	14.694618340350866	21.846885579842812	38.93175912147048
10	15.81483232486047	28.151496209897736	34.42526565618426	21.608405809057533
11	33.24774799072226	22.76875775392416	23.528872361286066	20.454621894067515
12	20.551809698473487	27.471911360153317	31.159749082220944	20.816529859152247
13	30.38192935084385	21.67116698343397	26.634820711822137	21.312082953900045
14	20.345979699393645	22.832946760882464	27.79428422752671	29.02678931219718
15	22.32625657199608	29.93965027620628	25.646824024393727	22.087269127403914
16	21.926399989846523	28.8371731453248	26.484136780015422	22.752290084813257
17	21.402480621138903	27.42945713805237	27.28810171244721	23.879960528361515
18	22.37543747203828	26.4586896304452	29.708404857169146	21.457468040347376
19	22.510002760476326	25.298686711320812	30.456779507746784	21.73453102045608
20	23.22245948921669	25.252773961410448	30.426033512817174	21.098733036555686
21	22.377690274556343	25.910973052039736	30.714170127838607	20.99716654556531
22	21.27791016077395	26.218274353271166	30.347280613523797	22.15653487243109
23	22.151267756684636	26.381650130250062	31.17358319345862	20.293498919606677
24	22.147714040036426	26.435907768361133	28.871758423418992	22.54461976818345
25	22.019113918829305	26.57269412970431	29.973505773203073	21.434686178263313
26	21.81525115575115	27.188565916684382	29.79417000092016	21.202012926644308
27	21.043682158121353	27.118887686689106	29.458312048051326	22.37911810713821
28	21.263536646116453	26.757043180830237	29.967762713262662	22.011657459790648
29	21.80049688573849	26.472714119360457	29.284402039579522	22.442386955321535
30	21.80887350355213	26.686603440124635	30.396302865501347	21.108220190821893
31	22.358715966023933	26.516881740559644	30.316947103562285	20.80745518985414
32	21.67687831376145	26.316604423742636	29.71541710162678	22.29110016086914
33	21.583339414842477	26.363151765911606	30.324879506794893	21.728629312451016
34	20.898138423609367	26.755520159409574	30.966261902571052	21.380079514410003
35	20.70147828266643	28.434429168398573	29.601698168884038	21.26239438005096
36	22.458473869077274	26.487658767050696	29.56546295091746	21.48840441295457
37	21.590002633557873	26.917626751871254	29.764217246313812	21.72815336825706
38	21.56024025662911	25.66449741879599	30.749739023933646	22.025523300641257
39	21.023216557781215	25.734841970662796	31.373162458791164	21.868779012764826
40	20.108324898544563	26.28046439461485	30.94738278287743	22.663827923963158
41	21.307418700799268	25.217490631831787	31.13135107864819	22.34373958872076
42	20.18885465616205	27.182442101388805	30.471248211243072	22.157455031206077
43	20.696369814984628	26.73943324565384	29.8219334122343	22.742263527127232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	164.0
1	301.5
2	439.0
3	790.0
4	1141.0
5	1141.0
6	2078.0
7	3015.0
8	3510.5
9	4006.0
10	6540.0
11	9074.0
12	9074.0
13	17961.0
14	26848.0
15	43104.5
16	59361.0
17	56191.0
18	53021.0
19	53021.0
20	59840.0
21	66659.0
22	57007.0
23	47355.0
24	54556.0
25	61757.0
26	61757.0
27	69570.0
28	77383.0
29	84795.0
30	92207.0
31	102931.0
32	113655.0
33	113655.0
34	127596.5
35	141538.0
36	154219.0
37	166900.0
38	176480.0
39	186060.0
40	186060.0
41	190121.0
42	194182.0
43	201052.0
44	207922.0
45	213839.5
46	219757.0
47	219757.0
48	263341.5
49	306926.0
50	282128.5
51	257331.0
52	236217.5
53	215104.0
54	215104.0
55	191417.5
56	167731.0
57	144944.5
58	122158.0
59	110811.5
60	99465.0
61	99465.0
62	88811.0
63	78157.0
64	68050.0
65	57943.0
66	49049.5
67	40156.0
68	40156.0
69	34202.5
70	28249.0
71	23298.0
72	18347.0
73	15037.5
74	11728.0
75	11728.0
76	9388.0
77	7048.0
78	5562.5
79	4077.0
80	3145.5
81	2214.0
82	2214.0
83	1687.5
84	1161.0
85	903.5
86	646.0
87	504.0
88	362.0
89	362.0
90	309.5
91	257.0
92	163.5
93	70.0
94	53.5
95	37.0
96	37.0
97	24.5
98	12.0
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3151630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.45001410454892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64746111329904	34.67375621581508
2	13.737169349299286	12.761834200630581
3	5.008583533229088	6.979463272869078
4	2.3304275947117423	4.329935785759618
5	1.2117451399845887	2.8142789421701377
6	0.7092995209258898	1.9768183650814422
7	0.4487750824740551	1.4591926237482977
8	0.3130542985130946	1.1633101265138328
9	0.22390630652542326	0.9360405986583028
>10	1.1477082553538522	10.052002105510432
>50	0.1189983077667232	3.8543392169156343
>100	0.08905943524900807	8.20714566048629
>500	0.00800005609995866	2.556277334428422
>1k	0.005128241089596729	3.9920930696806245
>5k	4.102592871677384E-4	1.3355465230887429
>10k+	2.735061914451589E-4	2.9079659586434987
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29779	0.944876143455926	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28222	0.8954731361232124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22406	0.7109337073197044	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11151	0.3538169137874687	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7817	0.2480303842773422	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7580	0.2405104660128251	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7203	0.22854840193804477	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6723	0.21331818773142786	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6509	0.20652805056431117	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6218	0.19729473320154967	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3643	0.11559097990563613	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3479	0.11038732338504202	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3394	0.10769030628595361	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3321	0.10537404454203064	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3182	0.10096362834469783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.855665163740668E-4
2	0.0	0.0	0.0	0.0	2.855665163740668E-4
3	0.0	0.0	0.0	0.0	2.855665163740668E-4
4	0.0	0.0	0.0	3.1729612930451864E-5	2.855665163740668E-4
5	0.0	0.0	0.0	3.1729612930451864E-5	2.855665163740668E-4
6	0.0	0.0	0.0	3.1729612930451864E-5	3.172961293045186E-4
7	0.0	0.0	0.0	3.1729612930451864E-5	3.4902574223497047E-4
8	0.0	0.0	0.0	3.1729612930451864E-5	3.8075535516542234E-4
9	0.0	0.0	0.0	1.586480646522593E-4	4.124849680958742E-4
10	0.0	0.0	0.0	2.2210729051316304E-4	4.124849680958742E-4
11	0.0	0.0	0.0	4.442145810263261E-4	4.124849680958742E-4
12	0.0	0.0	0.0	6.980514844699409E-4	4.124849680958742E-4
13	0.0	0.0	0.0	7.297810974003929E-4	4.7594419395677795E-4
14	0.0	0.0	0.0	8.249699361917484E-4	4.7594419395677795E-4
15	0.0	0.0	0.0	0.0011105364525658152	4.7594419395677795E-4
16	0.0	0.0	0.0	0.0014912918077312374	4.7594419395677795E-4
17	0.0	0.0	0.0	0.0020306952275489193	4.7594419395677795E-4
18	0.0	0.0	0.0	0.0022845321309925342	4.7594419395677795E-4
19	0.0	0.0	0.0	0.0028873947766711195	4.7594419395677795E-4
20	0.0	0.0	0.0	0.004822901165428683	6.028626456785854E-4
21	0.0	0.0	0.0	0.007964132845543417	6.028626456785854E-4
22	0.0	0.0	0.0	0.012818763623902552	6.028626456785854E-4
23	0.0	0.0	0.0	0.0153571326583387	6.345922586090372E-4
24	0.0	0.0	0.0	0.019799278468601963	6.345922586090372E-4
25	0.0	0.0	0.0	0.022591484406481725	6.980514844699409E-4
26	0.0	0.0	0.0	0.027858600152936733	6.980514844699409E-4
27	0.0	0.0	0.0	0.06545819147552219	6.980514844699409E-4
28	0.0	0.0	0.0	0.1468446486421312	6.980514844699409E-4
29	0.0	0.0	0.0	0.24574585214634967	6.980514844699409E-4
30	0.0	0.0	0.0	0.40071328169867654	7.297810974003929E-4
31	0.0	0.0	0.0	0.6168554049809146	7.297810974003929E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6980	0.0	29.472776	1
GTATTGG	1015	0.0	17.86207	1
GTATCAA	11960	0.0	17.154264	2
CAATGCG	140	3.479181E-8	15.857142	19
CTAATAC	885	0.0	15.677967	3
ATTGGAC	1315	0.0	15.61597	3
GGGTAAG	405	0.0	15.074074	1
TACTAGG	345	0.0	15.014493	2
GTATTAG	795	0.0	14.893083	1
TTGGACC	1750	0.0	14.694286	4
GCGACGA	265	0.0	14.660377	23
GGACCCT	1665	0.0	14.555555	6
TGGACCC	1680	0.0	14.535715	5
GACCCTC	1645	0.0	14.5076	7
CTCTAAT	770	0.0	14.4155855	1
TAATACT	1095	0.0	14.19178	4
TATACCG	145	8.92338E-7	14.034483	5
TAGTACT	520	0.0	13.875	4
CGTACAC	95	0.0012459157	13.631579	23
CCTATCG	95	0.0012459157	13.631579	3
>>END_MODULE