FastQCFastQC Report
Wed 25 May 2016
SRR2088111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118398
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161191.441257942163702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160981.4393802564024614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120841.0804740351824664No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55360.49499373210610176No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG43550.38939626143823575No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA41160.3680264092031638No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG35590.318223029726448No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG34980.31276879965808235No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA33360.2982837952142261No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27120.24248970402307585No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA23970.2143244176044664No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.18025783307910062No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC18520.1655940014198881No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.16219628432811933No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA17330.15495378210619118No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17150.15334433716798493No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA16150.14440297640017238No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG14970.1338521706941536No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14750.13188507132523483No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA14160.12660966847222546No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14130.12634142764919107No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG13450.12026130232707856No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.11364469535889726No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC12420.11105170073623163No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT11980.10711750199839414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39350.029.9479051
GTATTGG2400.021.5833341
ACGTTTA1052.2591848E-819.38095326
CAACCGT500.00703476618.56
ACCGTCG609.236601E-418.523
TATACCG852.7230091E-517.4117645
GTATACG752.0672036E-417.2666661
GGACCCT5050.017.2178236
GTATCAA68450.017.189192
CCCTAGT650.001579898217.0769231
ATTGGAC3100.016.7096793
TCACGTT1151.2420442E-616.08695624
TAAGCCG1059.342566E-615.85714436
GTTAGAA700.002592435315.8571422
ACACTAC700.002592435315.8571426
CGTCCCC700.002592435315.85714227
GCCAACG700.002592435315.85714224
TTTTTAC9250.015.61
TTCGTCT957.059402E-515.57894818
TGGACCC5650.015.38937955
Produced by FastQC (version 0.11.5)