##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088111_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1118398
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35109594258931	31.0	31.0	34.0	30.0	34.0
2	31.56149957349709	31.0	31.0	34.0	30.0	34.0
3	31.60824411345514	31.0	31.0	34.0	30.0	34.0
4	35.36086437922815	37.0	35.0	37.0	33.0	37.0
5	35.23741369351519	37.0	35.0	37.0	33.0	37.0
6	35.2835859863841	37.0	35.0	37.0	32.0	37.0
7	35.13070838824819	37.0	35.0	37.0	32.0	37.0
8	35.16408291145013	37.0	35.0	37.0	32.0	37.0
9	36.66442178902323	39.0	35.0	39.0	32.0	39.0
10	36.505577620846964	38.0	35.0	39.0	32.0	39.0
11	36.574676456860615	38.0	35.0	39.0	32.0	39.0
12	36.43082158587551	38.0	35.0	39.0	32.0	39.0
13	36.47664158913017	38.0	35.0	39.0	32.0	39.0
14	37.34903048825195	39.0	36.0	41.0	32.0	41.0
15	37.383023753619014	39.0	36.0	41.0	32.0	41.0
16	37.23528564965245	39.0	36.0	41.0	32.0	41.0
17	37.32117814946021	39.0	36.0	41.0	32.0	41.0
18	37.3271822732158	39.0	36.0	40.0	32.0	41.0
19	37.3774944161202	39.0	36.0	41.0	32.0	41.0
20	37.321526862530156	39.0	36.0	41.0	31.0	41.0
21	37.22196570451664	39.0	36.0	41.0	31.0	41.0
22	37.146974511756994	39.0	36.0	40.0	31.0	41.0
23	36.93860056974351	39.0	36.0	40.0	31.0	41.0
24	36.77920561374395	39.0	35.0	40.0	30.0	41.0
25	36.6396998206363	38.0	35.0	40.0	30.0	41.0
26	36.6959928397583	39.0	35.0	40.0	30.0	41.0
27	36.60766203086916	39.0	35.0	40.0	30.0	41.0
28	36.496434185325796	38.0	35.0	40.0	30.0	41.0
29	36.37917449780847	38.0	35.0	40.0	30.0	41.0
30	36.12717208006452	38.0	35.0	40.0	30.0	41.0
31	35.89793079029111	38.0	35.0	40.0	29.0	41.0
32	35.72814150239897	38.0	34.0	40.0	29.0	41.0
33	35.526252729350375	38.0	34.0	40.0	27.0	41.0
34	35.38629897406827	38.0	34.0	40.0	27.0	41.0
35	35.1720013805461	38.0	34.0	40.0	26.0	41.0
36	35.037973065044824	38.0	34.0	40.0	25.0	41.0
37	34.83192208855881	38.0	33.0	40.0	25.0	41.0
38	34.69496726567823	38.0	33.0	40.0	24.0	41.0
39	34.57905861777292	38.0	33.0	40.0	24.0	41.0
40	34.35281536626496	38.0	33.0	40.0	22.0	41.0
41	34.18610280061302	38.0	33.0	40.0	21.0	41.0
42	33.935740228433886	38.0	33.0	40.0	20.0	41.0
43	33.56237672098841	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	4.0
15	11.0
16	14.0
17	33.0
18	92.0
19	247.0
20	495.0
21	1021.0
22	1834.0
23	3319.0
24	5256.0
25	8074.0
26	11703.0
27	16180.0
28	22040.0
29	28520.0
30	35995.0
31	43678.0
32	53490.0
33	64396.0
34	78094.0
35	93768.0
36	117720.0
37	155303.0
38	193230.0
39	183880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.20187625514352	22.891135356107576	15.801083335270627	22.105905053478278
2	18.722136484507303	24.15383432373806	35.7847564105086	21.33927278124603
3	19.422334446234704	24.943892961181977	34.106284167174834	21.527488425408485
4	13.953619373425202	17.279447924620754	35.939620779007114	32.827311922946926
5	11.995819019704971	39.27564248147797	34.81631762574683	13.91222087307023
6	31.23887918254503	37.87748189821512	13.369837928894723	17.513800990345118
7	24.02168101158979	34.90045583057194	21.666437171740295	19.41142598609797
8	26.04144499543096	35.74764976332218	18.998066877802	19.212838363444856
9	23.636576603320105	15.27059240091631	23.302616778642307	37.790214217121274
10	16.489389287176838	28.706238745062134	33.32463040885266	21.479741558908366
11	31.922177972421267	23.260413555818232	24.0698749461283	20.7475335256322
12	20.531599663089526	28.568720616453174	31.21142920498785	19.68825051546945
13	31.37684437919238	22.115025241461446	27.37665839888841	19.13147198045776
14	19.636032968585422	24.408663105620718	28.982437379179864	26.972866546613993
15	21.60214878781972	30.523749148335387	26.794218158473104	21.07988390537179
16	20.527486637136334	29.551018510405065	27.937907614284	21.983587238174604
17	20.70345261704688	27.87504984808628	27.92351202344782	23.49798551141901
18	22.396678105647542	26.003980693813826	31.020352325379697	20.57898887515893
19	21.782138380075786	25.11976952748485	32.85315245556591	20.244939636873458
20	22.75343840028326	24.43477187906273	32.66627801551862	20.14551170513538
21	21.632549414430283	25.55127959813948	33.37953036396703	19.4366406234632
22	19.708726231627736	25.898830291184353	32.85646075905	21.53598271813791
23	21.266221863773005	26.136223419569777	33.7262763345428	18.87127838211442
24	20.841686054517265	26.029463572002097	31.389809352305708	21.73904102117493
25	20.763449147798905	26.76891410750019	32.279117094272344	20.18851965042856
26	20.524983056121346	27.467323797074027	32.11584784665209	19.89184530015254
27	19.143095749455917	27.423153474881033	31.96599064018355	21.467760135479498
28	19.843025470360285	27.025978229574804	31.88900552397268	21.24199077609223
29	19.966505662563776	26.493967263889957	31.22278473316297	22.316742340383296
30	20.616363763168387	26.86476549493114	32.95025563350435	19.568615108396116
31	21.647213246089496	26.582754976314337	32.42065883522682	19.349372942369353
32	20.79197208864823	26.59974356177318	31.609319759155508	20.998964590423085
33	20.253165688779845	26.560401574394803	32.299413983215274	20.88701875361007
34	19.270867794827957	26.807898440447854	33.28806024331231	20.633173521411877
35	19.00083869964002	28.87934348952698	31.76266409632349	20.357153714509504
36	21.46865427155628	26.421095173632285	31.570693080638556	20.539557474172877
37	20.44558377250317	27.28071759784978	31.673250488645365	20.60044814100168
38	20.195404498219773	25.660721853937506	33.12452275486902	21.0193508929737
39	19.68950230597694	25.196039334834293	33.978333294587436	21.136125064601334
40	18.360369027841607	25.871827381665565	33.45124007732489	22.316563513167942
41	20.009513607856952	25.18522028830524	33.38569990289682	21.41956620094099
42	18.53105960489915	27.650621692814187	32.437647420685664	21.380671281601003
43	18.95040942490956	27.81639452144943	31.281708300622856	21.951487753018156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	161.5
2	212.0
3	417.0
4	622.0
5	622.0
6	1098.0
7	1574.0
8	1905.0
9	2236.0
10	3675.5
11	5115.0
12	5115.0
13	9716.5
14	14318.0
15	23830.5
16	33343.0
17	31181.5
18	29020.0
19	29020.0
20	31610.5
21	34201.0
22	27715.0
23	21229.0
24	23299.5
25	25370.0
26	25370.0
27	26976.5
28	28583.0
29	29870.0
30	31157.0
31	34124.0
32	37091.0
33	37091.0
34	40997.0
35	44903.0
36	48939.5
37	52976.0
38	56024.5
39	59073.0
40	59073.0
41	60695.0
42	62317.0
43	65208.0
44	68099.0
45	73470.5
46	78842.0
47	78842.0
48	105110.5
49	131379.0
50	117296.0
51	103213.0
52	89886.5
53	76560.0
54	76560.0
55	65326.0
56	54092.0
57	44894.5
58	35697.0
59	31174.0
60	26651.0
61	26651.0
62	23229.5
63	19808.0
64	17208.0
65	14608.0
66	12027.0
67	9446.0
68	9446.0
69	7929.5
70	6413.0
71	5181.0
72	3949.0
73	3311.5
74	2674.0
75	2674.0
76	2131.5
77	1589.0
78	1242.5
79	896.0
80	691.0
81	486.0
82	486.0
83	378.5
84	271.0
85	205.0
86	139.0
87	104.0
88	69.0
89	69.0
90	49.5
91	30.0
92	26.5
93	23.0
94	17.0
95	11.0
96	11.0
97	6.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1118398.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.391657201955965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.36034858390522	39.990994812678835
2	11.839769347774403	11.93251196646547
3	3.74648897104194	5.6637536381896245
4	1.6148411050660072	3.2549807760845595
5	0.8250119965559031	2.078686085897317
6	0.5112135035011436	1.5456537375264328
7	0.34095771669436337	1.2026997066016785
8	0.253499276603774	1.0219398918048956
9	0.18339575067422792	0.8317454220243969
>10	1.1399818970684936	11.042592639736624
>50	0.10906607624941977	3.798143631230011
>100	0.06261762512300416	5.896843890787267
>500	0.007649311651529164	2.809924624083074
>1k	0.0044472742160053275	4.462030501128026
>5k	1.778909686402131E-4	0.4962592585632584
>10k+	5.336729059206394E-4	3.971239417198503
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16119	1.441257942163702	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16098	1.4393802564024614	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12084	1.0804740351824664	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5536	0.49499373210610176	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4355	0.38939626143823575	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4116	0.3680264092031638	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3559	0.318223029726448	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3498	0.31276879965808235	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3336	0.2982837952142261	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2712	0.24248970402307585	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2397	0.2143244176044664	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2016	0.18025783307910062	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1852	0.1655940014198881	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1814	0.16219628432811933	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1733	0.15495378210619118	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1715	0.15334433716798493	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1615	0.14440297640017238	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1497	0.1338521706941536	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1475	0.13188507132523483	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1416	0.12660966847222546	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1413	0.12634142764919107	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1345	0.12026130232707856	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1271	0.11364469535889726	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1242	0.11105170073623163	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1198	0.10711750199839414	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	8.941360767812531E-5	0.0	0.0	8.941360767812531E-5	0.0
3	8.941360767812531E-5	0.0	0.0	8.941360767812531E-5	0.0
4	8.941360767812531E-5	0.0	0.0	1.7882721535625063E-4	0.0
5	8.941360767812531E-5	0.0	0.0	1.7882721535625063E-4	0.0
6	8.941360767812531E-5	0.0	0.0	1.7882721535625063E-4	0.0
7	8.941360767812531E-5	0.0	0.0	1.7882721535625063E-4	0.0
8	8.941360767812531E-5	0.0	0.0	1.7882721535625063E-4	0.0
9	8.941360767812531E-5	0.0	0.0	2.6824082303437594E-4	0.0
10	8.941360767812531E-5	0.0	0.0	5.364816460687519E-4	0.0
11	8.941360767812531E-5	0.0	0.0	6.258952537468773E-4	0.0
12	8.941360767812531E-5	0.0	0.0	0.0010729632921375038	0.0
13	8.941360767812531E-5	0.0	0.0	0.0012517905074937545	0.0
14	8.941360767812531E-5	0.0	0.0	0.0013412041151718798	0.0
15	8.941360767812531E-5	0.0	0.0	0.0017882721535625063	0.0
16	8.941360767812531E-5	0.0	0.0	0.0025929946226656343	0.0
17	8.941360767812531E-5	0.0	0.0	0.00402361234551564	0.0
18	8.941360767812531E-5	0.0	0.0	0.004470680383906266	0.0
19	8.941360767812531E-5	0.0	0.0	0.0051859892453312685	0.0
20	8.941360767812531E-5	0.0	0.0	0.00840487912174378	0.0
21	8.941360767812531E-5	0.0	0.0	0.0141273500131438	0.0
22	1.7882721535625063E-4	0.0	0.0	0.025572291795943843	0.0
23	1.7882721535625063E-4	0.0	0.0	0.03245713958715949	0.0
24	1.7882721535625063E-4	0.0	0.0	0.04086201870890327	0.0
25	1.7882721535625063E-4	0.0	0.0	0.0476574528924408	0.0
26	1.7882721535625063E-4	0.0	0.0	0.05802943138310333	0.0
27	1.7882721535625063E-4	0.0	0.0	0.12526846435705358	0.0
28	1.7882721535625063E-4	0.0	0.0	0.268956131895801	0.0
29	1.7882721535625063E-4	0.0	0.0	0.44867748332883284	0.0
30	1.7882721535625063E-4	0.0	0.0	0.7792395909148622	0.0
31	1.7882721535625063E-4	0.0	0.0	1.1981423428868794	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3935	0.0	29.947905	1
GTATTGG	240	0.0	21.583334	1
ACGTTTA	105	2.2591848E-8	19.380953	26
CAACCGT	50	0.007034766	18.5	6
ACCGTCG	60	9.236601E-4	18.5	23
TATACCG	85	2.7230091E-5	17.411764	5
GTATACG	75	2.0672036E-4	17.266666	1
GGACCCT	505	0.0	17.217823	6
GTATCAA	6845	0.0	17.18919	2
CCCTAGT	65	0.0015798982	17.076923	1
ATTGGAC	310	0.0	16.709679	3
TCACGTT	115	1.2420442E-6	16.086956	24
TAAGCCG	105	9.342566E-6	15.857144	36
GTTAGAA	70	0.0025924353	15.857142	2
ACACTAC	70	0.0025924353	15.857142	6
CGTCCCC	70	0.0025924353	15.857142	27
GCCAACG	70	0.0025924353	15.857142	24
TTTTTAC	925	0.0	15.6	1
TTCGTCT	95	7.059402E-5	15.578948	18
TGGACCC	565	0.0	15.3893795	5
>>END_MODULE