FastQCFastQC Report
Wed 25 May 2016
SRR2088110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036733
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT276871.359382894075954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT265331.3027235283171628No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT216761.0642533901105349No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99830.49014770222704696No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG78370.3847828851400748No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA73410.36043015947598434No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG72920.3580243458519109No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG67570.3317567889360068No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA62080.30480185669893894No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55110.2705803853524247No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA42910.21068053593671826No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34160.167719578363978No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34110.16747408717784804No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32860.16133680752459945No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32680.16045303925453164No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32580.15996205688227177No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27710.13605121535321518No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG26730.13123958810506825No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA26370.12947205156493266No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG26250.1288828727182208No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG24990.12269649482774621No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22990.11287684738254843No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC22620.11106021260518684No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22050.10826161308330547No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT21130.1037445752585145No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT20990.10305719993735066No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC20370.10001310922933934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66850.029.9708291
GTATTGG5750.018.6608681
ACGTTTA1705.456968E-1217.41176426
GTATCAA115900.017.2549612
TAGTACT2750.016.8181824
CTGTGCG2200.015.9772739
CGGTATA1059.350102E-615.85714428
TTAGTAC957.063956E-515.5789483
GTCGTCA1201.9371873E-615.41666724
CATTGCG1353.97622E-715.07407529
TGGACCC11800.015.0508475
GGACCCT11450.015.0262016
TAATACT3950.014.9873424
TTGGACC12250.014.951024
CTAATAC3350.014.9104483
ATTGGAC7450.014.8993283
TGCCGGT1001.09402215E-414.819
AGTACTG5050.014.6534665
ATAGTAC2301.8189894E-1214.4782613
GACCCTC11400.014.4429827
Produced by FastQC (version 0.11.5)