##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088110_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2036733
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.627259439504343	31.0	31.0	34.0	30.0	34.0
2	31.825010936632342	31.0	31.0	34.0	30.0	34.0
3	31.894296896058542	31.0	31.0	34.0	30.0	34.0
4	35.57920797669601	37.0	35.0	37.0	33.0	37.0
5	35.48966310262563	37.0	35.0	37.0	33.0	37.0
6	35.523587038654554	37.0	35.0	37.0	33.0	37.0
7	35.37811632648953	37.0	35.0	37.0	33.0	37.0
8	35.423253808918496	37.0	35.0	37.0	33.0	37.0
9	36.9670261148614	39.0	37.0	39.0	33.0	39.0
10	36.82586917381905	39.0	37.0	39.0	32.0	39.0
11	36.868782997084054	39.0	37.0	39.0	33.0	39.0
12	36.74317252187695	39.0	35.0	39.0	32.0	39.0
13	36.75660629056435	39.0	35.0	39.0	32.0	39.0
14	37.72743359095178	39.0	37.0	41.0	32.0	41.0
15	37.73182788318351	39.0	37.0	41.0	32.0	41.0
16	37.61663261703915	39.0	36.0	41.0	32.0	41.0
17	37.66643050414561	39.0	37.0	41.0	32.0	41.0
18	37.65655586667472	39.0	37.0	41.0	32.0	41.0
19	37.69973874827972	39.0	37.0	41.0	32.0	41.0
20	37.623929106073305	39.0	37.0	41.0	32.0	41.0
21	37.5384908085645	39.0	36.0	41.0	32.0	41.0
22	37.42996995678864	39.0	36.0	41.0	32.0	41.0
23	37.201157441844366	39.0	36.0	41.0	31.0	41.0
24	37.05294312018316	39.0	36.0	41.0	31.0	41.0
25	36.89711611684006	39.0	36.0	40.0	30.0	41.0
26	37.05378515495158	39.0	36.0	41.0	31.0	41.0
27	36.99300841101902	39.0	36.0	41.0	31.0	41.0
28	36.899077100434866	39.0	35.0	41.0	31.0	41.0
29	36.797197767208566	39.0	35.0	40.0	30.0	41.0
30	36.5410571734243	39.0	35.0	40.0	30.0	41.0
31	36.31194663218007	38.0	35.0	40.0	30.0	41.0
32	36.139901499116476	38.0	35.0	40.0	30.0	41.0
33	35.94352033378946	38.0	35.0	40.0	29.0	41.0
34	35.794331903101686	38.0	35.0	40.0	28.0	41.0
35	35.57300245049302	38.0	34.0	40.0	27.0	41.0
36	35.46457881322687	38.0	34.0	40.0	27.0	41.0
37	35.26575697452734	38.0	34.0	40.0	26.0	41.0
38	35.145712766474546	38.0	34.0	40.0	25.0	41.0
39	35.01661042463592	38.0	34.0	40.0	24.0	41.0
40	34.811968971878	38.0	33.0	40.0	24.0	41.0
41	34.63104147671786	38.0	33.0	40.0	23.0	41.0
42	34.379709564287516	38.0	33.0	40.0	22.0	41.0
43	34.02658767742262	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	3.0
15	7.0
16	17.0
17	62.0
18	136.0
19	312.0
20	688.0
21	1534.0
22	2874.0
23	4990.0
24	8120.0
25	12239.0
26	18286.0
27	25567.0
28	34502.0
29	44883.0
30	56590.0
31	70408.0
32	86005.0
33	106300.0
34	131199.0
35	160121.0
36	206575.0
37	286461.0
38	369977.0
39	408866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.38708068264225	21.99718863493644	14.83989310331791	22.775837579103396
2	18.711534599773262	23.663877395809855	35.54844940402105	22.07613860039583
3	19.130686250971532	24.44674878837825	33.957175535526744	22.46538942512347
4	13.511982179303816	16.541736202045136	36.34388994531929	33.60239167333175
5	11.961116160046506	39.024408206672156	35.156498176245975	13.85797745703536
6	31.606499231858077	37.87148340013148	13.085760381945008	17.43625698606543
7	25.011574909426027	34.443886361147975	21.166986541682196	19.377552187743806
8	26.798259762079763	34.94738878390049	18.716984504105348	19.537366949914396
9	24.033047041512066	14.811072438066256	22.643223240356	38.512657280065675
10	16.320450446867607	28.069609516809518	33.38886343963593	22.22107659668695
11	32.50759917966665	22.653337477224554	24.012622174826056	20.826441168282734
12	21.14990035512755	27.81734277394239	30.714187868512955	20.318569002417107
13	31.632472199350627	21.49054392500146	26.91496627196594	19.962017603681975
14	20.13611995288533	23.700602877254898	28.226576581221003	27.936700588638768
15	21.907191566101204	29.951446753207218	26.473867708727656	21.667493971963925
16	21.28305477448443	29.09924864967573	27.076744963625572	22.540951612214265
17	21.199440476488572	27.241862335416574	27.21034126711749	24.34835592097737
18	22.97861329884673	25.480070289036412	30.41792910509134	21.123387307025514
19	22.49489746569629	24.60612166641381	31.71098028067498	21.188000587214916
20	23.576973515919857	23.97422735331533	31.806329057367854	20.642470073396954
21	22.140850077059685	25.137364593199013	32.47087369822162	20.250911631519696
22	20.439988943076976	25.537760717776948	31.908502489035133	22.113747850110936
23	21.906062306645005	25.596089423601427	32.98994026217477	19.507908007578802
24	21.74295796258027	25.59908441607221	30.21623354656698	22.44172407478054
25	21.466485788760725	26.18688851214175	31.5245542739279	20.82207142516962
26	21.184809201795225	27.03491326550903	31.231339601214298	20.548937931481444
27	19.8216948416901	27.12250452072019	30.841352302928264	22.21444833466144
28	20.530329699572796	26.342186236487553	31.05895569031385	22.0685283736258
29	20.867339999891986	26.161308330547005	30.26714841857033	22.70420325099068
30	21.213973554707465	26.317244331976745	32.15345359455559	20.315328518760193
31	22.194072566212654	26.07028019873002	31.69620171126996	20.039445523787357
32	21.48587959246499	26.043816248865216	30.73957165715879	21.730732501511
33	20.977369149515425	25.930006534975377	31.64440307099654	21.44822124451266
34	20.005567740101426	26.665841816281272	32.35004293640845	20.978547507208848
35	19.629769832373707	28.867652264680743	30.719048593998327	20.78352930894722
36	22.21945635485849	26.205054859915368	30.619575565378476	20.95591321984767
37	21.209063730984866	27.08420789568392	30.605484371294615	21.101244002036594
38	20.944522428811236	25.321973965168727	32.08078820346113	21.652715402558904
39	20.43699395060619	25.281369722982838	32.81662348476703	21.465012841643947
40	19.0099045873956	25.88498345143914	32.38711210551408	22.717999855651183
41	20.75181184769923	24.912740157890113	32.43036765251017	21.905080341900486
42	19.444767674506185	27.339420532784615	31.533490153103035	21.682321639606172
43	19.904719960839245	27.31177822522638	30.40496717046368	22.378534643470697
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	238.5
2	331.0
3	686.0
4	1041.0
5	1041.0
6	1843.5
7	2646.0
8	3087.0
9	3528.0
10	5659.5
11	7791.0
12	7791.0
13	15578.5
14	23366.0
15	38626.0
16	53886.0
17	50692.0
18	47498.0
19	47498.0
20	51899.5
21	56301.0
22	45060.5
23	33820.0
24	37417.0
25	41014.0
26	41014.0
27	43912.5
28	46811.0
29	49660.0
30	52509.0
31	57096.0
32	61683.0
33	61683.0
34	69271.0
35	76859.0
36	84498.0
37	92137.0
38	97847.0
39	103557.0
40	103557.0
41	107265.0
42	110973.0
43	116348.5
44	121724.0
45	131758.5
46	141793.0
47	141793.0
48	189858.5
49	237924.0
50	214682.0
51	191440.0
52	170152.0
53	148864.0
54	148864.0
55	128670.0
56	108476.0
57	91091.5
58	73707.0
59	66047.0
60	58387.0
61	58387.0
62	51082.0
63	43777.0
64	37904.0
65	32031.0
66	26896.0
67	21761.0
68	21761.0
69	18477.0
70	15193.0
71	12475.0
72	9757.0
73	8193.5
74	6630.0
75	6630.0
76	5213.5
77	3797.0
78	3138.0
79	2479.0
80	1930.0
81	1381.0
82	1381.0
83	1072.5
84	764.0
85	592.0
86	420.0
87	335.5
88	251.0
89	251.0
90	206.0
91	161.0
92	116.5
93	72.0
94	51.0
95	30.0
96	30.0
97	19.5
98	9.0
99	8.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2036733.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.02705684815712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82358144592321	33.382891307505325
2	12.976048607771123	11.425944594375787
3	4.617091858041403	6.098308971214568
4	2.0742747570634132	3.6529685059171286
5	1.1656101000447328	2.565919106872778
6	0.6836827597352545	1.80603238373814
7	0.46486176904635657	1.432654687263718
8	0.32334841057133407	1.13888630831883
9	0.23327002264288604	0.924317329308232
>10	1.3781594831897668	11.480189034480821
>50	0.1468654546924516	4.4869944805177875
>100	0.09901363832943776	8.358544886116015
>500	0.006816482439927944	2.0647544600237455
>1k	0.006257754371081391	4.942018925463336
>5k	7.822192963851739E-4	2.505620470228492
>10k+	3.352368413079317E-4	3.733954548655219
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27687	1.359382894075954	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26533	1.3027235283171628	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21676	1.0642533901105349	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9983	0.49014770222704696	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7837	0.3847828851400748	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7341	0.36043015947598434	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7292	0.3580243458519109	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6757	0.3317567889360068	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6208	0.30480185669893894	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5511	0.2705803853524247	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4291	0.21068053593671826	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3416	0.167719578363978	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3411	0.16747408717784804	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3286	0.16133680752459945	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3268	0.16045303925453164	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3258	0.15996205688227177	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2771	0.13605121535321518	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2673	0.13123958810506825	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2637	0.12947205156493266	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2625	0.1288828727182208	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2499	0.12269649482774621	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2299	0.11287684738254843	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2262	0.11106021260518684	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2205	0.10826161308330547	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2113	0.1037445752585145	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2099	0.10305719993735066	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2037	0.10001310922933934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.9098237225988876E-5	0.0	0.0	0.0	0.0
6	9.819647445197775E-5	0.0	0.0	0.0	0.0
7	9.819647445197775E-5	0.0	0.0	0.0	0.0
8	1.4729471167796663E-4	0.0	0.0	0.0	0.0
9	1.4729471167796663E-4	0.0	0.0	2.9458942335593326E-4	0.0
10	1.4729471167796663E-4	0.0	0.0	4.418841350338999E-4	0.0
11	1.4729471167796663E-4	0.0	0.0	7.364735583898331E-4	0.0
12	1.4729471167796663E-4	0.0	0.0	0.0015220453540056552	0.0
13	1.4729471167796663E-4	0.0	0.0	0.001620241828457633	0.0
14	1.4729471167796663E-4	0.0	0.0	0.001963929489039555	0.0
15	1.4729471167796663E-4	0.0	0.0	0.002454911861299444	0.0
16	1.4729471167796663E-4	0.0	0.0	0.003240483656915266	0.0
17	1.4729471167796663E-4	0.0	0.0	0.0041733501642090545	0.0
18	1.4729471167796663E-4	0.0	0.0	0.005008020197050865	0.0
19	1.4729471167796663E-4	0.0	0.0	0.006382770839378554	0.0
20	1.4729471167796663E-4	0.0	0.0	0.010654317478039585	0.0
21	1.4729471167796663E-4	0.0	0.0	0.01782266011303396	0.0
22	1.4729471167796663E-4	0.0	0.0	0.027347718134875805	0.0
23	1.4729471167796663E-4	0.0	0.0	0.03304311365309051	0.0
24	1.4729471167796663E-4	0.0	0.0	0.04212628753989846	0.0
25	1.4729471167796663E-4	0.0	0.0	0.04693791478804536	0.0
26	1.4729471167796663E-4	0.0	0.0	0.05611928514930528	0.0
27	1.4729471167796663E-4	0.0	0.0	0.11277865090809645	0.0
28	1.4729471167796663E-4	0.0	0.0	0.2313508938088596	0.0
29	1.4729471167796663E-4	0.0	0.0	0.3723610311218996	0.0
30	1.963929489039555E-4	0.0	0.0	0.609260025737296	0.0
31	1.963929489039555E-4	0.0	0.0	0.9156330260274665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6685	0.0	29.970829	1
GTATTGG	575	0.0	18.660868	1
ACGTTTA	170	5.456968E-12	17.411764	26
GTATCAA	11590	0.0	17.254961	2
TAGTACT	275	0.0	16.818182	4
CTGTGCG	220	0.0	15.977273	9
CGGTATA	105	9.350102E-6	15.857144	28
TTAGTAC	95	7.063956E-5	15.578948	3
GTCGTCA	120	1.9371873E-6	15.416667	24
CATTGCG	135	3.97622E-7	15.074075	29
TGGACCC	1180	0.0	15.050847	5
GGACCCT	1145	0.0	15.026201	6
TAATACT	395	0.0	14.987342	4
TTGGACC	1225	0.0	14.95102	4
CTAATAC	335	0.0	14.910448	3
ATTGGAC	745	0.0	14.899328	3
TGCCGGT	100	1.09402215E-4	14.8	19
AGTACTG	505	0.0	14.653466	5
ATAGTAC	230	1.8189894E-12	14.478261	3
GACCCTC	1140	0.0	14.442982	7
>>END_MODULE