FastQCFastQC Report
Wed 25 May 2016
SRR2088109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522826
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT214341.4075147127774281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203861.338695294143914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT140440.9222327435964451No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75680.4969707635672099No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG56710.372399735754446No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG55840.36668667332971727No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA52860.34711779284041644No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA47570.3123797466026979No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA43340.2846024430893615No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG40160.263720214916215No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.16745182969032574No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA24660.16193576941817384No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA22320.14656960151717924No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21810.14322056492337273No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC21420.14065953693987363No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20920.1373761677302594No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA19650.12903640993783924No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19300.12673805149110928No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19130.12562170595984046No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC17160.11268523127396038No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG16690.10959886421692301No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16560.10874518822242331No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT16120.1058558233179628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48850.030.1453441
GTATTGG5050.020.5148531
GTATCAA78450.018.7240282
GTTAGCC801.6172326E-518.53
CGACTAT609.2386955E-418.536
GATCGGA500.00703590818.49999833
ATTGGAC6450.018.356593
TCTATAC1054.7997855E-717.6190473
TTGGACC9850.017.2791884
GGACCCT9550.017.0471216
TGGACCC9950.016.5477395
CTAGTCC904.4474844E-516.4444453
AACCGGC1201.936407E-615.4166667
TGCGGTA855.3655717E-415.23529436
GACCCTC10850.015.1751167
CTAGACA1353.97431E-715.0740754
CATTGCG750.0041052214.829
GGACTAG1252.9600888E-614.7999991
TAAACCG1001.0937346E-414.7999995
AATGCGT1152.2106662E-514.47826135
Produced by FastQC (version 0.11.5)