##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1522826
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04108282889838	31.0	31.0	33.0	30.0	34.0
2	31.24828903630487	31.0	31.0	34.0	30.0	34.0
3	31.26986865209814	31.0	31.0	34.0	28.0	34.0
4	35.048268810750535	35.0	35.0	37.0	32.0	37.0
5	34.901814127155696	35.0	35.0	37.0	32.0	37.0
6	34.98163020594605	35.0	35.0	37.0	32.0	37.0
7	34.82943881966817	35.0	35.0	37.0	32.0	37.0
8	34.87939856556166	35.0	35.0	37.0	32.0	37.0
9	36.324356164131686	38.0	35.0	39.0	32.0	39.0
10	36.09315378119365	38.0	35.0	39.0	31.0	39.0
11	36.20673274556647	38.0	35.0	39.0	32.0	39.0
12	36.04121219364524	37.0	35.0	39.0	31.0	39.0
13	36.10397116939164	38.0	35.0	39.0	31.0	39.0
14	36.91238526266297	38.0	36.0	40.0	31.0	41.0
15	36.945807991195316	38.0	36.0	40.0	31.0	41.0
16	36.76816261345682	38.0	36.0	40.0	31.0	41.0
17	36.83583153951929	38.0	36.0	40.0	31.0	41.0
18	36.832627627844545	38.0	36.0	40.0	31.0	41.0
19	36.880869514967564	39.0	36.0	40.0	31.0	41.0
20	36.80200232987879	39.0	36.0	40.0	31.0	41.0
21	36.70275395875826	39.0	35.0	40.0	30.0	41.0
22	36.594567599975306	38.0	35.0	40.0	30.0	41.0
23	36.37233932176099	38.0	35.0	40.0	30.0	41.0
24	36.23138099822304	38.0	34.0	40.0	29.0	41.0
25	36.07769567895478	38.0	34.0	40.0	29.0	41.0
26	36.14237345566729	38.0	35.0	40.0	30.0	41.0
27	36.037633321206755	38.0	34.0	40.0	29.0	41.0
28	35.9173464335387	38.0	34.0	40.0	29.0	41.0
29	35.795442814871826	38.0	34.0	40.0	28.0	41.0
30	35.547823585885716	38.0	34.0	40.0	27.0	41.0
31	35.34264190393387	38.0	34.0	40.0	27.0	41.0
32	35.18345300119646	38.0	34.0	40.0	27.0	41.0
33	34.99206540996805	38.0	33.0	40.0	26.0	41.0
34	34.82167168146591	37.0	33.0	40.0	25.0	41.0
35	34.60503892105861	37.0	33.0	40.0	25.0	41.0
36	34.49537832950055	37.0	33.0	40.0	24.0	41.0
37	34.304984942468806	37.0	33.0	40.0	24.0	41.0
38	34.16402136554012	37.0	33.0	40.0	23.0	41.0
39	34.057068896906145	37.0	33.0	40.0	23.0	41.0
40	33.84191299596934	37.0	33.0	40.0	21.0	41.0
41	33.67112132311899	37.0	32.0	40.0	20.0	41.0
42	33.42597315780004	37.0	32.0	40.0	19.0	41.0
43	33.06249761955732	37.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	12.0
16	38.0
17	59.0
18	194.0
19	500.0
20	977.0
21	1934.0
22	3415.0
23	5687.0
24	9224.0
25	13793.0
26	19723.0
27	26883.0
28	35303.0
29	44874.0
30	56014.0
31	67763.0
32	80892.0
33	96944.0
34	115216.0
35	137461.0
36	170925.0
37	217044.0
38	262518.0
39	155428.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.61091155522693	23.93228116672555	15.72464615130028	21.732161126747247
2	20.01607537565027	24.8808465313831	33.70568929083165	21.39738880213498
3	20.778670708275275	25.29849109484603	33.043039716947305	20.87979847993139
4	14.92875745488979	17.837822574607998	35.3649727546023	31.868447215899913
5	11.737716587449912	39.9559109182533	35.13631892284476	13.170053571452023
6	31.716098884573814	38.39644187845492	12.90580801746227	16.981651219508993
7	24.863444674572143	34.01202763808866	21.607393096781905	19.51713459055729
8	26.901037938674545	35.52362515481086	18.750927551801716	18.82440935471288
9	23.97483363168215	14.472763139058568	22.263278930094444	39.28912429916484
10	16.243681155956097	28.567873151627303	33.66976923167847	21.518676460738128
11	32.91603899591943	22.536717917871115	23.61129899279366	20.935944093415795
12	20.372386602277608	28.0963813331267	31.092915408589032	20.438316656006663
13	31.440295870966217	21.390362392026404	27.17506793290895	19.994273804098434
14	19.96235945538098	23.14276220658171	28.392015896760363	28.502862441276942
15	21.959829947741895	30.09634718608692	26.468815215920927	21.47500765025026
16	21.08238236016459	28.705643323662716	27.51837701746621	22.693597298706482
17	21.17293768296575	27.088649655311904	27.868318507826896	23.870094153895455
18	22.33012832720219	25.583421874856356	30.722945366049697	21.36350443189176
19	22.208118327372926	24.363387543947898	32.13046007882713	21.298034049852053
20	23.029682971002597	24.240786537660902	32.22298542315406	20.506545068182444
21	22.091361718279042	25.07955603594895	32.722451547320574	20.10663069845143
22	20.39175848061433	25.410913656583222	32.21714102596094	21.980186836841504
23	21.607918435855442	25.76788155705248	33.28581203630618	19.338387970785895
24	21.539690023679658	25.74837834394737	30.4420859638593	22.26984566851367
25	21.382941977612678	26.263473305551656	31.587719148477895	20.76586556835778
26	21.29278065911667	26.91837412810131	31.309814778576143	20.479030434205878
27	20.20802113964432	26.858616808486328	30.970248734917842	21.96311331695151
28	20.598873410356795	26.364798079360348	31.581086742674476	21.455241767608378
29	21.082185358012012	25.851870141434414	30.69956777727725	22.36637672327633
30	21.151595783103257	26.111715980683282	32.30960070290368	20.42708753330978
31	21.919050502158488	26.089980076515634	32.01035443313944	19.98061498818644
32	21.392398080936363	25.801962929448273	30.99690969289991	21.80872929671545
33	21.03950155828703	26.003693133686973	31.691013943812358	21.26579136421364
34	20.034856247529266	26.736409806504486	32.53720385651414	20.69153008945211
35	19.756886210243323	28.79199593387557	30.920538525084286	20.530579330796822
36	22.19990990434889	26.15676380623919	30.79478548435606	20.848540805055862
37	21.197234615116894	27.135010828551653	30.73818019918231	20.929574357149143
38	21.20137166032101	25.272421143321694	32.07641582163688	21.44979137472042
39	20.643658566375937	25.3847123702905	32.79481700470047	21.176812058633093
40	19.31474771247667	26.17409999566595	32.01994187123151	22.491210420625862
41	21.105365944631888	25.081460390090527	31.973646365375952	21.839527299901633
42	19.825902631029415	27.44069250196674	31.063430753086696	21.66997411391715
43	20.509697102623676	27.236007265439387	29.98937501723769	22.26492061469925
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	153.0
2	196.0
3	330.0
4	464.0
5	464.0
6	998.0
7	1532.0
8	1907.0
9	2282.0
10	3779.0
11	5276.0
12	5276.0
13	11495.5
14	17715.0
15	29678.5
16	41642.0
17	37705.5
18	33769.0
19	33769.0
20	37942.0
21	42115.0
22	33680.0
23	25245.0
24	27189.0
25	29133.0
26	29133.0
27	31606.0
28	34079.0
29	36988.0
30	39897.0
31	44049.0
32	48201.0
33	48201.0
34	54487.5
35	60774.0
36	67177.5
37	73581.0
38	78109.5
39	82638.0
40	82638.0
41	84987.5
42	87337.0
43	91309.0
44	95281.0
45	102126.0
46	108971.0
47	108971.0
48	140787.0
49	172603.0
50	154672.5
51	136742.0
52	122927.0
53	109112.0
54	109112.0
55	94132.0
56	79152.0
57	66065.0
58	52978.0
59	47556.0
60	42134.0
61	42134.0
62	37203.0
63	32272.0
64	27912.5
65	23553.0
66	19432.0
67	15311.0
68	15311.0
69	12965.5
70	10620.0
71	8848.0
72	7076.0
73	5901.5
74	4727.0
75	4727.0
76	3775.5
77	2824.0
78	2193.0
79	1562.0
80	1250.0
81	938.0
82	938.0
83	684.0
84	430.0
85	347.0
86	264.0
87	207.0
88	150.0
89	150.0
90	116.5
91	83.0
92	60.0
93	37.0
94	24.5
95	12.0
96	12.0
97	9.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1522826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.192743514383785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.68476330880108	38.402273601454304
2	11.38915966723891	10.977497013752188
3	3.657317719517338	5.287685244219303
4	1.5801453056012233	3.0460614971318933
5	0.866022860908492	2.086800880667791
6	0.5250315206429131	1.5181625646786485
7	0.34941244736857946	1.178740111973695
8	0.2618923502030491	1.0097048689371777
9	0.1797725414721173	0.7797358783885203
>10	1.2883214455010021	12.084874782522231
>50	0.11990714806717781	3.987798469571311
>100	0.08431510183305013	7.520130838697768
>500	0.008472471925458925	2.901186103471829
>1k	0.004509541508712008	3.952588644545712
>5k	5.466110919650919E-4	1.58773967195429
>10k+	4.0995831897381896E-4	3.6790198280332835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21434	1.4075147127774281	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20386	1.338695294143914	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	14044	0.9222327435964451	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7568	0.4969707635672099	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5671	0.372399735754446	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5584	0.36668667332971727	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5286	0.34711779284041644	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4757	0.3123797466026979	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4334	0.2846024430893615	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4016	0.263720214916215	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2550	0.16745182969032574	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2466	0.16193576941817384	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2232	0.14656960151717924	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2181	0.14322056492337273	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2142	0.14065953693987363	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2092	0.1373761677302594	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1965	0.12903640993783924	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1930	0.12673805149110928	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1913	0.12562170595984046	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1716	0.11268523127396038	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1669	0.10959886421692301	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1656	0.10874518822242331	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1612	0.1058558233179628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	6.566738419228461E-5	0.0
8	0.0	0.0	0.0	6.566738419228461E-5	0.0
9	0.0	0.0	0.0	1.9700215257685382E-4	0.0
10	0.0	0.0	0.0	2.6266953676913846E-4	0.0
11	0.0	0.0	0.0	4.596716893459923E-4	0.0
12	0.0	0.0	0.0	8.536759944996999E-4	0.0
13	0.0	0.0	0.0	9.193433786919845E-4	0.0
14	6.566738419228461E-5	0.0	0.0	9.850107628842691E-4	0.0
15	6.566738419228461E-5	0.0	0.0	0.001182012915461123	0.0
16	6.566738419228461E-5	0.0	0.0	0.0019700215257685383	0.0
17	6.566738419228461E-5	0.0	0.0	0.0028893649044605227	0.0
18	6.566738419228461E-5	0.0	0.0	0.0034803713621910843	0.0
19	6.566738419228461E-5	0.0	0.0	0.0040713778199216455	0.0
20	6.566738419228461E-5	0.0	0.0	0.0072890796453435915	0.0
21	6.566738419228461E-5	0.0	0.0	0.012017131307188083	0.0
22	6.566738419228461E-5	0.0	0.0	0.020750893404761935	0.0
23	6.566738419228461E-5	0.0	0.0	0.024296932151145305	0.0
24	6.566738419228461E-5	0.0	0.0	0.03007566196006635	0.0
25	6.566738419228461E-5	0.0	0.0	0.03342469855387287	0.0
26	6.566738419228461E-5	0.0	0.0	0.04084511296760102	0.0
27	6.566738419228461E-5	0.0	0.0	0.07965453702524122	0.0
28	6.566738419228461E-5	0.0	0.0	0.15930907405048245	0.0
29	6.566738419228461E-5	0.0	0.0	0.25347610298221857	0.0
30	1.3133476838456923E-4	0.0	0.0	0.41055248597016336	0.0
31	1.3133476838456923E-4	0.0	0.0	0.6118886859037079	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4885	0.0	30.145344	1
GTATTGG	505	0.0	20.514853	1
GTATCAA	7845	0.0	18.724028	2
GTTAGCC	80	1.6172326E-5	18.5	3
CGACTAT	60	9.2386955E-4	18.5	36
GATCGGA	50	0.007035908	18.499998	33
ATTGGAC	645	0.0	18.35659	3
TCTATAC	105	4.7997855E-7	17.619047	3
TTGGACC	985	0.0	17.279188	4
GGACCCT	955	0.0	17.047121	6
TGGACCC	995	0.0	16.547739	5
CTAGTCC	90	4.4474844E-5	16.444445	3
AACCGGC	120	1.936407E-6	15.416666	7
TGCGGTA	85	5.3655717E-4	15.235294	36
GACCCTC	1085	0.0	15.175116	7
CTAGACA	135	3.97431E-7	15.074075	4
CATTGCG	75	0.00410522	14.8	29
GGACTAG	125	2.9600888E-6	14.799999	1
TAAACCG	100	1.0937346E-4	14.799999	5
AATGCGT	115	2.2106662E-5	14.478261	35
>>END_MODULE