FastQCFastQC Report
Wed 25 May 2016
SRR2088108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082397
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT281281.350751081566099No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT261631.25638867132444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT220521.0589719443506689No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99020.4755097130854491No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG78330.3761530582304911No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG76820.36890179922464356No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA72190.34666780637889894No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG66950.3215044969811232No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA65530.31468543222065726No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63830.3065217631412262No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA39650.19040557588202442No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC36900.17719964060647417No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA35200.1690359715270431No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33970.16312931684016066No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32850.1577508995643002No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32210.1546775182638085No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30050.14430485637464902No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG26060.12514424482939612No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.12360755417915029No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG24910.11962176280507512No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG24090.11568399301382012No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC23090.11088183473180187No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC22640.10872086350489366No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22630.10867284192207347No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT21980.10555143903876159No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT21960.10545539587312122No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21920.10526330954184049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64350.030.3877241
GTATTGG7050.021.255321
ATTGGAC7900.019.4367083
GTGTACG500.007036752518.51
TTGGACC12200.018.0450824
GTATCAA110300.017.7116972
TGGACCC12300.017.1463415
GTATTAG2700.017.129631
CGCATAC650.001580516717.07692337
ATAACGC650.001580516717.0769233
TATACCG1201.0423719E-716.9583345
GGACCCT12050.016.5809146
TATCGGC803.3842144E-416.18752
GATACTG6950.015.438848536
CGCCGGA1353.9763472E-715.07407314
GACCCTC13350.014.9662917
TATTGGA9900.014.9494952
ACGACGC750.004105894414.827
CGAATTA1252.9613548E-614.79999915
TGATACT7200.014.64583435
Produced by FastQC (version 0.11.5)