##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088108_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2082397
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.613574164772615	31.0	31.0	34.0	30.0	34.0
2	31.81277969570644	31.0	31.0	34.0	30.0	34.0
3	31.882653980004772	31.0	31.0	34.0	30.0	34.0
4	35.569711251024664	37.0	35.0	37.0	33.0	37.0
5	35.48073590194377	37.0	35.0	37.0	33.0	37.0
6	35.51396971855031	37.0	35.0	37.0	33.0	37.0
7	35.36908236037605	37.0	35.0	37.0	33.0	37.0
8	35.413045639232095	37.0	35.0	37.0	33.0	37.0
9	36.95600022474101	39.0	37.0	39.0	33.0	39.0
10	36.81119690433668	39.0	37.0	39.0	32.0	39.0
11	36.85582528211479	39.0	37.0	39.0	32.0	39.0
12	36.72303119914214	39.0	35.0	39.0	32.0	39.0
13	36.75113294919269	39.0	35.0	39.0	32.0	39.0
14	37.71629040956167	39.0	37.0	41.0	32.0	41.0
15	37.723815391589596	39.0	37.0	41.0	32.0	41.0
16	37.60281012698347	39.0	36.0	41.0	32.0	41.0
17	37.645191574901425	39.0	37.0	41.0	32.0	41.0
18	37.63510608207753	39.0	37.0	41.0	32.0	41.0
19	37.67346524221846	39.0	37.0	41.0	32.0	41.0
20	37.60090943273545	39.0	37.0	41.0	32.0	41.0
21	37.51653503150456	39.0	36.0	41.0	32.0	41.0
22	37.40557540180859	39.0	36.0	41.0	32.0	41.0
23	37.17892601650886	39.0	36.0	41.0	31.0	41.0
24	37.02176098025497	39.0	36.0	41.0	31.0	41.0
25	36.876054854093624	39.0	36.0	40.0	30.0	41.0
26	37.03666976085732	39.0	36.0	41.0	31.0	41.0
27	36.96705863483284	39.0	36.0	41.0	31.0	41.0
28	36.88344489547382	39.0	35.0	41.0	30.0	41.0
29	36.78831846184949	39.0	35.0	40.0	30.0	41.0
30	36.537994916435245	39.0	35.0	40.0	30.0	41.0
31	36.30680413004821	38.0	35.0	40.0	30.0	41.0
32	36.149046027246484	38.0	35.0	40.0	30.0	41.0
33	35.95835520316251	38.0	35.0	40.0	29.0	41.0
34	35.7999247982013	38.0	35.0	40.0	28.0	41.0
35	35.58469830680701	38.0	34.0	40.0	27.0	41.0
36	35.47967846669007	38.0	34.0	40.0	27.0	41.0
37	35.28678873432876	38.0	34.0	40.0	26.0	41.0
38	35.17909985463867	38.0	34.0	40.0	25.0	41.0
39	35.05736994434779	38.0	34.0	40.0	24.0	41.0
40	34.856359762331586	38.0	33.0	40.0	24.0	41.0
41	34.68295862892619	38.0	33.0	40.0	23.0	41.0
42	34.429398428829856	38.0	33.0	40.0	22.0	41.0
43	34.09005631491017	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	5.0
15	9.0
16	26.0
17	64.0
18	135.0
19	345.0
20	717.0
21	1528.0
22	2918.0
23	5073.0
24	8191.0
25	13024.0
26	18504.0
27	25778.0
28	35023.0
29	45866.0
30	58154.0
31	71766.0
32	88494.0
33	109113.0
34	134146.0
35	164416.0
36	211913.0
37	292028.0
38	378221.0
39	416936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46452237493619	21.862401837882018	14.791992112935237	22.881083674246554
2	18.662915860904526	23.72866461102278	35.53424250995367	22.074177018119023
3	19.162772516479805	24.498162454133386	33.764647183029936	22.574417846356866
4	13.503188873207176	16.381218374786364	36.54538495781544	33.57020779419102
5	11.894369805565413	39.031798451496044	35.192472905022434	13.881358837916114
6	31.66327074040157	37.874334240781174	13.198924124458497	17.26347089435876
7	25.133968210672602	34.231176860128016	21.239465865538605	19.395389063660772
8	26.89842522823458	34.87913207712074	18.85442593319141	19.36801676145327
9	24.110724323940154	14.769902184837955	22.38631730645021	38.733056184771684
10	16.154508482292282	28.14996371969418	33.503121642991225	22.19240615502231
11	32.59253638955492	22.626137091054204	23.95561461143096	20.825711907959914
12	20.871332411639088	27.715272352005886	31.050755451530136	20.362639784824893
13	31.727235488718048	21.393134930563193	26.83887846553755	20.04075111518121
14	20.194996439199635	23.383293387380025	28.274531705529732	28.147178467890612
15	21.921564427916483	30.020260305791837	26.16556785281577	21.89260741347591
16	21.259154714494883	29.090322354479	26.92565346569362	22.724869465332496
17	21.256945721685156	27.100115876079343	27.219545552553136	24.42339284968236
18	23.103183494789896	25.564865873318105	30.1793558096751	21.152594822216898
19	22.411336551099524	24.649766591096704	31.62590994896746	21.312986908836308
20	23.601311373383655	24.114181877903203	31.558871819350486	20.725634929362652
21	22.306409392637427	25.010792850738834	32.23112595725023	20.45167179937351
22	20.54007953334547	25.349921268614967	31.872788906246026	22.237210291793545
23	21.982455794932473	25.58047288773466	32.77328962729009	19.66378169004277
24	21.696487269238286	25.41143691620762	30.291246097646123	22.600829716907967
25	21.550597700630572	26.059248068451886	31.37177973268306	21.018374498234486
26	21.364177916122625	26.83527684682604	31.063000955149285	20.737544281902057
27	19.931790143762214	27.050797710523018	30.766227573320553	22.251184572394216
28	20.680830792591422	26.247636737855462	30.93680023549784	22.134732234055274
29	20.860863706584286	26.08445939943248	30.15625742833859	22.89841946564464
30	21.247101297207017	26.251766593977997	31.887435489006176	20.613696619808806
31	22.266599500479494	25.9046185717709	31.612896100023196	20.215885827726414
32	21.607023060444284	26.053197349016543	30.553828112506885	21.78595147803229
33	21.28148475050627	25.882768751587715	31.256864084994362	21.57888241291166
34	20.077391582873005	26.71166929264689	32.04211300726999	21.16882611721012
35	19.827391222711135	28.840754188562506	30.60290617014911	20.728948418577247
36	22.249695903326792	26.19193170178405	30.488230630374513	21.070141764514645
37	21.39712072193727	27.061122350829358	30.45519178139423	21.086565145839145
38	21.109471440844374	25.410716592465317	31.891757431459993	21.588054535230313
39	20.569852914693982	25.10385867824435	32.77886013089723	21.54742827616444
40	19.208825214404364	25.64510993821063	32.294034230744664	22.85203061664034
41	21.01693385074988	24.82787864177676	32.25177523786291	21.903412269610453
42	19.59703168992272	27.202978106480174	31.44323584791949	21.756754355677614
43	20.060295899389022	27.23760166769353	30.319290702013113	22.382811730904336
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	172.0
1	259.5
2	347.0
3	653.0
4	959.0
5	959.0
6	1761.5
7	2564.0
8	3016.0
9	3468.0
10	5609.0
11	7750.0
12	7750.0
13	15364.0
14	22978.0
15	38201.0
16	53424.0
17	50420.0
18	47416.0
19	47416.0
20	51315.0
21	55214.0
22	44555.0
23	33896.0
24	38218.5
25	42541.0
26	42541.0
27	46403.5
28	50266.0
29	53079.0
30	55892.0
31	61176.5
32	66461.0
33	66461.0
34	73518.0
35	80575.0
36	87398.5
37	94222.0
38	98729.0
39	103236.0
40	103236.0
41	107158.0
42	111080.0
43	115506.0
44	119932.0
45	130897.5
46	141863.0
47	141863.0
48	190522.0
49	239181.0
50	216595.0
51	194009.0
52	173745.0
53	153481.0
54	153481.0
55	133007.0
56	112533.0
57	95288.5
58	78044.0
59	69324.0
60	60604.0
61	60604.0
62	53317.0
63	46030.0
64	40394.5
65	34759.0
66	29325.5
67	23892.0
68	23892.0
69	20234.5
70	16577.0
71	13961.0
72	11345.0
73	9435.0
74	7525.0
75	7525.0
76	5944.0
77	4363.0
78	3467.0
79	2571.0
80	2037.5
81	1504.0
82	1504.0
83	1165.5
84	827.0
85	631.0
86	435.0
87	330.5
88	226.0
89	226.0
90	187.5
91	149.0
92	102.0
93	55.0
94	37.0
95	19.0
96	19.0
97	13.0
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2082397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.16605102141754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.08351692036163	35.12475524034299
2	12.827557825116026	11.843953780689805
3	4.5885100772512715	6.3550017101601215
4	2.1267407505283673	3.9273288799288175
5	1.114545160832259	2.572707438032804
6	0.7111024303635163	1.9697274648969663
7	0.42720390173724354	1.3805621986905157
8	0.3296192232624054	1.2173774303017781
9	0.23851987819277412	0.9910368779642915
>10	1.3416016794422114	11.694294274270122
>50	0.1270617299307948	4.057929259457328
>100	0.07267327239045807	6.3690732838853705
>500	0.004684603455030875	1.5847758775353593
>1k	0.005621524146037049	4.7304899120524
>5k	7.287160930048027E-4	2.51194786326431
>10k+	3.123068970020583E-4	3.669038508527125
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28128	1.350751081566099	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26163	1.25638867132444	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22052	1.0589719443506689	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9902	0.4755097130854491	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7833	0.3761530582304911	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7682	0.36890179922464356	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7219	0.34666780637889894	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6695	0.3215044969811232	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6553	0.31468543222065726	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6383	0.3065217631412262	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3965	0.19040557588202442	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3690	0.17719964060647417	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3520	0.1690359715270431	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3397	0.16312931684016066	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3285	0.1577508995643002	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3221	0.1546775182638085	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3005	0.14430485637464902	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2606	0.12514424482939612	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2574	0.12360755417915029	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2491	0.11962176280507512	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2409	0.11568399301382012	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2309	0.11088183473180187	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2264	0.10872086350489366	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2263	0.10867284192207347	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2198	0.10555143903876159	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2196	0.10545539587312122	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2192	0.10526330954184049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.8021582820182706E-5	0.0	0.0	0.0	0.0
6	4.8021582820182706E-5	0.0	0.0	0.0	0.0
7	4.8021582820182706E-5	0.0	0.0	0.0	0.0
8	4.8021582820182706E-5	0.0	0.0	0.0	0.0
9	4.8021582820182706E-5	0.0	0.0	9.604316564036541E-5	0.0
10	4.8021582820182706E-5	0.0	0.0	4.321942453816443E-4	0.0
11	4.8021582820182706E-5	0.0	0.0	7.683453251229233E-4	0.0
12	4.8021582820182706E-5	0.0	0.0	0.0013446043189651157	0.0
13	4.8021582820182706E-5	0.0	0.0	0.0015847122330660292	0.0
14	4.8021582820182706E-5	0.0	0.0	0.0018728417299871253	0.0
15	4.8021582820182706E-5	0.0	0.0	0.0023530575581889525	0.0
16	4.8021582820182706E-5	0.0	0.0	0.0033134892145926065	0.0
17	4.8021582820182706E-5	0.0	0.0	0.0040818345397155295	0.0
18	4.8021582820182706E-5	0.0	0.0	0.0051863309445797316	0.0
19	4.8021582820182706E-5	0.0	0.0	0.006098741018163203	0.0
20	4.8021582820182706E-5	0.0	0.0	0.009604316564036541	0.0
21	4.8021582820182706E-5	0.0	0.0	0.015174820171177734	0.0
22	4.8021582820182706E-5	0.0	0.0	0.02381870507881062	0.0
23	4.8021582820182706E-5	0.0	0.0	0.02828471228108761	0.0
24	4.8021582820182706E-5	0.0	0.0	0.03558399286975538	0.0
25	4.8021582820182706E-5	0.0	0.0	0.04000197848921219	0.0
26	4.8021582820182706E-5	0.0	0.0	0.048405755482744166	0.0
27	4.8021582820182706E-5	0.0	0.0	0.10156564766468641	0.0
28	4.8021582820182706E-5	0.0	0.0	0.21062266224932133	0.0
29	4.8021582820182706E-5	0.0	0.0	0.34152949701713936	0.0
30	4.8021582820182706E-5	0.0	0.0	0.5605559362599927	0.0
31	4.8021582820182706E-5	0.0	0.0	0.8354794979055387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6435	0.0	30.387724	1
GTATTGG	705	0.0	21.25532	1
ATTGGAC	790	0.0	19.436708	3
GTGTACG	50	0.0070367525	18.5	1
TTGGACC	1220	0.0	18.045082	4
GTATCAA	11030	0.0	17.711697	2
TGGACCC	1230	0.0	17.146341	5
GTATTAG	270	0.0	17.12963	1
CGCATAC	65	0.0015805167	17.076923	37
ATAACGC	65	0.0015805167	17.076923	3
TATACCG	120	1.0423719E-7	16.958334	5
GGACCCT	1205	0.0	16.580914	6
TATCGGC	80	3.3842144E-4	16.1875	2
GATACTG	695	0.0	15.4388485	36
CGCCGGA	135	3.9763472E-7	15.074073	14
GACCCTC	1335	0.0	14.966291	7
TATTGGA	990	0.0	14.949495	2
ACGACGC	75	0.0041058944	14.8	27
CGAATTA	125	2.9613548E-6	14.799999	15
TGATACT	720	0.0	14.645834	35
>>END_MODULE