FastQCFastQC Report
Wed 25 May 2016
SRR2088107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311486
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119560.9116376385260688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT118450.9031739568702982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80670.6151037830369519No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44460.3390047625365425No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG23620.1801010456840561No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA21610.1647749194425255No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG19420.1480763042838429No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG17590.13412266695946432No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15620.11910153825507859No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.11063785659930796No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13670.10423290831926531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30400.027.9325661
GGTATCG555.143624E-420.18181835
TCACGTT1254.129106E-1019.24000224
GTGTTAC801.6169532E-518.51
CGAACTA1306.9667294E-1018.524
CGCTTAG500.007035399318.49999825
GTATCAA47200.017.9904652
TCTAGCA1258.581992E-917.7600023
CGTAAGC953.6071706E-617.5263162
GACCGTG852.7235905E-517.4117647
GCGAACT1502.5102054E-1017.26666623
ACGTTTA1401.869921E-917.17857226
CGCCGGA650.001580094817.07692314
TAAGACT2900.016.5862064
CGATCGT957.060886E-515.57894813
TATACCG750.00410481614.85
GTAAGAC1001.0935507E-414.7999993
TCCGACG1405.9961894E-714.53571523
ACTACCC1152.2102036E-514.4782613
CTCTTAG908.275111E-414.3888881
Produced by FastQC (version 0.11.5)