##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088107_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1311486
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.428341591141653	31.0	31.0	34.0	30.0	34.0
2	31.645695036012583	31.0	31.0	34.0	30.0	34.0
3	31.71659933846034	31.0	31.0	34.0	30.0	34.0
4	35.44927280962206	37.0	35.0	37.0	33.0	37.0
5	35.31333083235353	37.0	35.0	37.0	33.0	37.0
6	35.33539511668443	37.0	35.0	37.0	33.0	37.0
7	35.22807029583236	37.0	35.0	37.0	32.0	37.0
8	35.24594391400289	37.0	35.0	37.0	32.0	37.0
9	36.78221727109553	39.0	37.0	39.0	32.0	39.0
10	36.58320561561465	38.0	35.0	39.0	32.0	39.0
11	36.64195119124413	39.0	35.0	39.0	32.0	39.0
12	36.5212850156235	38.0	35.0	39.0	32.0	39.0
13	36.55024300678772	38.0	35.0	39.0	32.0	39.0
14	37.538573801016554	39.0	36.0	41.0	32.0	41.0
15	37.554924718982896	39.0	36.0	41.0	32.0	41.0
16	37.425707174914564	39.0	36.0	41.0	32.0	41.0
17	37.40229708895101	39.0	36.0	41.0	32.0	41.0
18	37.383881337658195	39.0	36.0	41.0	32.0	41.0
19	37.41047254793418	39.0	36.0	41.0	32.0	41.0
20	37.33010874687187	39.0	36.0	40.0	31.0	41.0
21	37.23960682767487	39.0	36.0	40.0	31.0	41.0
22	37.112837651335965	39.0	36.0	40.0	31.0	41.0
23	36.91517332247542	39.0	36.0	40.0	31.0	41.0
24	36.76012553698629	39.0	35.0	40.0	30.0	41.0
25	36.626151556326185	39.0	35.0	40.0	30.0	41.0
26	36.79116666132921	39.0	36.0	40.0	30.0	41.0
27	36.698951418467296	39.0	35.0	40.0	30.0	41.0
28	36.63606397628339	39.0	35.0	40.0	30.0	41.0
29	36.55510466752981	39.0	35.0	40.0	30.0	41.0
30	36.34690191126707	38.0	35.0	40.0	30.0	41.0
31	36.184013401591784	38.0	35.0	40.0	30.0	41.0
32	36.01030434179244	38.0	35.0	40.0	29.0	41.0
33	35.83956062054799	38.0	35.0	40.0	28.0	41.0
34	35.7103118142321	38.0	34.0	40.0	27.0	41.0
35	35.537986680757555	38.0	34.0	40.0	27.0	41.0
36	35.44612676002641	38.0	34.0	40.0	27.0	41.0
37	35.27819282859291	38.0	34.0	40.0	26.0	41.0
38	35.197174045319585	38.0	34.0	40.0	25.0	41.0
39	35.108598948063495	38.0	34.0	40.0	25.0	41.0
40	34.95554279649192	38.0	33.0	40.0	24.0	41.0
41	34.81312267153443	38.0	33.0	40.0	24.0	41.0
42	34.65727731748566	38.0	33.0	40.0	23.0	41.0
43	34.326667612159035	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	2.0
15	3.0
16	22.0
17	65.0
18	117.0
19	282.0
20	566.0
21	1183.0
22	2079.0
23	3667.0
24	5774.0
25	8722.0
26	12338.0
27	17503.0
28	23427.0
29	30280.0
30	37954.0
31	47365.0
32	58097.0
33	70772.0
34	87036.0
35	106633.0
36	136342.0
37	181408.0
38	249220.0
39	230626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.013683714504005	22.958765857965695	14.667102813144783	23.360447614385514
2	19.20066245465068	24.078640564977437	34.56094842034151	22.15974856003038
3	19.487131391414014	25.249678608845233	32.80706008298983	22.45612991675092
4	14.568969855568417	18.065919117703125	36.180714090733716	31.18439693599474
5	12.742568353760545	38.834802658968535	35.02789964971033	13.394729337560598
6	31.390194024183256	38.57715598946538	14.459475739733401	15.573174246617958
7	25.39752616497622	33.39364659630373	22.356395722104544	18.852431516615503
8	25.929136872219754	35.73442644450646	19.497958803982655	18.838477879291123
9	24.71776290406455	14.976217817041126	22.030048357359515	38.27597092153481
10	16.308599558058567	28.603584026058986	33.93699970872735	21.15081670715509
11	33.14034614170491	23.517292597862273	23.15914923986989	20.183212020562934
12	20.159803459587064	27.87059869491554	31.134453589287265	20.835144256210132
13	30.225408429826928	22.09752906245282	26.19044351216864	21.48661899555161
14	20.1756633315186	23.668418877517563	28.011507557076477	28.144410233887363
15	22.85720167809645	30.328421347997615	25.088868657385593	21.725508316520344
16	21.74914562564907	28.924898931441128	27.02087555642988	22.305079886479916
17	21.36530622515223	28.175138735754707	27.54745380431053	22.91210123478253
18	22.146709915317434	27.035134191291405	29.324521954485217	21.493633938905944
19	22.328107200534355	26.431391566513103	30.546265838903352	20.694235394049194
20	22.72384150497985	26.147591358199783	30.23623584239557	20.8923312944248
21	21.887157011207133	26.733872873976544	30.555797011939127	20.823173102877192
22	21.449409296019933	26.858235619747372	30.201847370082486	21.49050771415021
23	21.935499120844597	26.79616862093839	30.522933527311768	20.745398730905247
24	21.68059742917576	27.03566793698141	29.43912477906741	21.844609854775424
25	21.68296116008863	27.285308421134502	29.987662849622488	21.04406756915438
26	21.367669956065104	27.7623245692291	29.795285653068355	21.07471982163744
27	20.98672803217114	27.653592947236948	29.61160088632284	21.748078134269065
28	21.1031608419762	27.650847969402648	29.596198510697025	21.649792677924125
29	21.307890438784707	27.344477943340607	29.544196430613823	21.803435187260863
30	21.520854969096124	27.57436983696357	30.255984432925704	20.648790761014606
31	21.992457410906407	27.249852457441403	30.04256240630857	20.715127725343617
32	21.584065708669403	27.275472250561577	29.65437679090741	21.48608524986161
33	21.350361345832134	27.23490757812131	29.884421183298944	21.530309892747614
34	20.936022191620804	27.04009040127001	30.696172128410065	21.327715278699124
35	20.83499175744156	27.9477630718132	29.947403174719362	21.26984199602588
36	21.739766951381867	26.980463382758185	29.87542375595317	21.404345909906777
37	21.289819334708874	26.958579809468038	30.144431583714958	21.60716927210813
38	21.093400920787563	26.38152446918991	30.831819783055252	21.693254826967273
39	20.73037760220086	26.19692470983297	31.556036435005787	21.516661252960382
40	20.060221763709258	26.294371422950757	31.341699415777217	22.303707397562764
41	20.705444053539267	25.666838990275153	31.45843722311942	22.169279733066155
42	19.89666683441531	27.111154827424766	30.708600778048716	22.2835775601112
43	20.185194504554378	26.68728450017766	30.50966613444596	22.617854860822
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	207.5
2	255.0
3	416.5
4	578.0
5	578.0
6	1050.0
7	1522.0
8	1730.0
9	1938.0
10	3134.5
11	4331.0
12	4331.0
13	8153.0
14	11975.0
15	18811.5
16	25648.0
17	23941.0
18	22234.0
19	22234.0
20	25957.5
21	29681.0
22	26026.0
23	22371.0
24	26070.5
25	29770.0
26	29770.0
27	33155.0
28	36540.0
29	38771.0
30	41002.0
31	45810.5
32	50619.0
33	50619.0
34	55624.0
35	60629.0
36	66087.5
37	71546.0
38	74179.0
39	76812.0
40	76812.0
41	78940.5
42	81069.0
43	83204.0
44	85339.0
45	87748.0
46	90157.0
47	90157.0
48	103934.0
49	117711.0
50	108977.5
51	100244.0
52	90844.5
53	81445.0
54	81445.0
55	74387.0
56	67329.0
57	60426.0
58	53523.0
59	48447.0
60	43371.0
61	43371.0
62	38159.5
63	32948.0
64	28315.5
65	23683.0
66	19799.0
67	15915.0
68	15915.0
69	13581.0
70	11247.0
71	9474.0
72	7701.0
73	6366.5
74	5032.0
75	5032.0
76	4101.5
77	3171.0
78	2499.5
79	1828.0
80	1446.0
81	1064.0
82	1064.0
83	785.5
84	507.0
85	398.0
86	289.0
87	227.5
88	166.0
89	166.0
90	130.0
91	94.0
92	60.0
93	26.0
94	19.5
95	13.0
96	13.0
97	7.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1311486.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.23961523592695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.71396781927959	40.07508162100448
2	12.87758212785567	13.454398710564593
3	4.499745447420442	7.051949124985734
4	2.0266157548168606	4.234785090508019
5	1.085087160174961	2.83422678724923
6	0.617694796280679	1.9360883094562173
7	0.39727420959786386	1.4527416296784583
8	0.26190964964846314	1.0945647459369716
9	0.19634026535554666	0.92310659257442
>10	1.1337734154962282	11.294449032879589
>50	0.11857251925922586	4.302018599470162
>100	0.0652885970431913	5.990275208910723
>500	0.00424521179449055	1.443405294897414
>1k	0.0014638661360312243	1.475905811471263
>5k	1.4638661360312245E-4	0.6168980404735067
>10k+	2.927732272062449E-4	1.8201053999392505
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11956	0.9116376385260688	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11845	0.9031739568702982	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8067	0.6151037830369519	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4446	0.3390047625365425	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2362	0.1801010456840561	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2161	0.1647749194425255	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1942	0.1480763042838429	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1759	0.13412266695946432	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1562	0.11910153825507859	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1451	0.11063785659930796	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1367	0.10423290831926531	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.5249876857244377E-4	0.0
11	0.0	0.0	0.0	2.2874815285866567E-4	0.0
12	0.0	0.0	0.0	4.5749630571733133E-4	0.0
13	0.0	0.0	0.0	4.5749630571733133E-4	0.0
14	0.0	0.0	0.0	5.337456900035532E-4	0.0
15	0.0	0.0	0.0	5.337456900035532E-4	0.0
16	0.0	0.0	0.0	6.099950742897751E-4	0.0
17	0.0	0.0	0.0	7.624938428622189E-4	0.0
18	0.0	0.0	0.0	9.149926114346627E-4	0.0
19	0.0	0.0	0.0	0.0012962395328657722	0.0
20	0.0	0.0	0.0	0.0019062346071555471	0.0
21	0.0	0.0	0.0	0.003583721061452429	0.0
22	0.0	0.0	0.0	0.005947451974325307	0.0
23	0.0	0.0	0.0	0.007777437197194633	0.0
24	0.0	0.0	0.0	0.011513657027219504	0.0
25	0.0	0.0	0.0	0.015097378088671934	0.0
26	0.0	0.0	0.0	0.023637309128728787	0.0
27	0.0	0.0	0.0	0.07708812751337032	0.0
28	0.0	0.0	0.0	0.1723998578711477	0.0
29	0.0	0.0	0.0	0.28639268737904944	0.0
30	7.624938428622188E-5	0.0	0.0	0.45902129340305575	0.0
31	7.624938428622188E-5	0.0	0.0	0.7045443108046903	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3040	0.0	27.932566	1
GGTATCG	55	5.143624E-4	20.181818	35
TCACGTT	125	4.129106E-10	19.240002	24
GTGTTAC	80	1.6169532E-5	18.5	1
CGAACTA	130	6.9667294E-10	18.5	24
CGCTTAG	50	0.0070353993	18.499998	25
GTATCAA	4720	0.0	17.990465	2
TCTAGCA	125	8.581992E-9	17.760002	3
CGTAAGC	95	3.6071706E-6	17.526316	2
GACCGTG	85	2.7235905E-5	17.411764	7
GCGAACT	150	2.5102054E-10	17.266666	23
ACGTTTA	140	1.869921E-9	17.178572	26
CGCCGGA	65	0.0015800948	17.076923	14
TAAGACT	290	0.0	16.586206	4
CGATCGT	95	7.060886E-5	15.578948	13
TATACCG	75	0.004104816	14.8	5
GTAAGAC	100	1.0935507E-4	14.799999	3
TCCGACG	140	5.9961894E-7	14.535715	23
ACTACCC	115	2.2102036E-5	14.478261	3
CTCTTAG	90	8.275111E-4	14.388888	1
>>END_MODULE