FastQCFastQC Report
Wed 25 May 2016
SRR2088106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623982
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT348212.1441740117809185No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT330382.034382154482008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT228741.40851314854475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112970.6956357890666276No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG90930.5599199991132907No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA82670.5090573663993813No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77060.47451264853920794No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63500.3910141861178264No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG63300.38978264537414825No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55680.3428609430400091No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA44120.2716778880554095No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40870.25166535097063886No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC37740.2323917383320751No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35760.220199484969661No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34990.21545805310649993No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30250.18627053748132674No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29370.1808517582091427No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28770.17715713597810812No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT27960.17216939596621145No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG27790.17112258633408498No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27460.16909054410701596No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG26890.16558065298753313No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC24330.14981693146845224No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.14119614626270488No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT22330.1375015240316703No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC20940.12894231586310687No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA20860.12844969956563557No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT20660.1272181588219574No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA20460.12598661807827918No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT20180.12426246103712972No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG19450.11976733732270432No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19170.11804318028155485No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG18900.11638060027758929No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG18620.11465644323643981No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.11299386323247425No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA18000.11083866693103742No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA17730.10917608692707186No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT17090.10523515654730163No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA17030.10486569432419818No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC16590.10215630468810614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69250.031.5768971
GGTCTAG450.003826478720.5555551
CGCTTAT450.003826478720.5555551
ATGCGAT555.144352E-420.18181828
TGCGATT759.269639E-619.73333429
GTATCAA115150.019.0060792
GTATTGG3700.019.0000021
CGCGCTA500.00703610418.49999816
TTATGCG1258.58563E-917.764
GTACACT650.00158031417.0769234
CGACCGC1301.3942554E-817.07692310
GCGCTAC650.00158031417.07692317
TATACCG1201.0419535E-716.9583325
CTAGTAC1655.4569682E-1116.8181823
GTATACT1107.812487E-716.8181824
TAGCACC1005.880798E-616.654
TTTTTAC12050.016.5809141
ATTGGAC4800.016.5729163
GGACCCT8500.016.5411786
GTGCGCG904.4477765E-516.44444511
Produced by FastQC (version 0.11.5)