##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088106_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1623982
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.132203435752366	31.0	31.0	33.0	30.0	34.0
2	31.33581960883803	31.0	31.0	34.0	30.0	34.0
3	31.333092977631527	31.0	31.0	34.0	28.0	34.0
4	35.10245125869622	35.0	35.0	37.0	32.0	37.0
5	34.99482321848395	35.0	35.0	37.0	32.0	37.0
6	35.09409525475036	36.0	35.0	37.0	32.0	37.0
7	34.90426925914203	36.0	35.0	37.0	32.0	37.0
8	35.00818358824174	36.0	35.0	37.0	32.0	37.0
9	36.42060010517358	38.0	35.0	39.0	32.0	39.0
10	36.21993224062828	38.0	35.0	39.0	32.0	39.0
11	36.32481456075252	38.0	35.0	39.0	32.0	39.0
12	36.146901258757794	38.0	35.0	39.0	31.0	39.0
13	36.21563908959582	38.0	35.0	39.0	32.0	39.0
14	36.96643682011254	39.0	36.0	40.0	31.0	41.0
15	37.01316455477955	38.0	36.0	40.0	31.0	41.0
16	36.83534546565171	38.0	36.0	40.0	31.0	41.0
17	36.97461363488019	38.0	36.0	40.0	31.0	41.0
18	36.95814670359647	38.0	36.0	40.0	31.0	41.0
19	37.05055290021687	39.0	36.0	40.0	31.0	41.0
20	36.98355092605706	39.0	36.0	40.0	31.0	41.0
21	36.907287149734415	39.0	36.0	40.0	31.0	41.0
22	36.795213247437474	39.0	36.0	40.0	30.0	41.0
23	36.552418684443545	38.0	35.0	40.0	30.0	41.0
24	36.38366004056695	38.0	35.0	40.0	30.0	41.0
25	36.22061266688917	38.0	35.0	40.0	30.0	41.0
26	36.30976759594626	38.0	35.0	40.0	30.0	41.0
27	36.20115801776128	38.0	35.0	40.0	30.0	41.0
28	36.06784865842109	38.0	35.0	40.0	30.0	41.0
29	35.94568104818896	38.0	35.0	40.0	29.0	41.0
30	35.62320087291608	38.0	34.0	40.0	27.0	41.0
31	35.30007105990091	38.0	34.0	40.0	27.0	41.0
32	35.122840031478184	37.0	34.0	40.0	27.0	41.0
33	34.8787018575329	37.0	33.0	40.0	25.0	41.0
34	34.69122009973017	37.0	33.0	40.0	25.0	41.0
35	34.437161865094566	37.0	33.0	40.0	24.0	41.0
36	34.29589428946873	37.0	33.0	40.0	24.0	41.0
37	34.0599132256392	37.0	33.0	40.0	23.0	41.0
38	33.86678115890447	37.0	33.0	40.0	22.0	41.0
39	33.67996997503667	37.0	33.0	40.0	21.0	41.0
40	33.38691992891547	37.0	32.0	40.0	18.0	41.0
41	33.13379335485246	37.0	31.0	40.0	17.0	41.0
42	32.793184284062264	36.0	31.0	40.0	15.0	41.0
43	32.41256368605071	36.0	30.0	40.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	5.0
15	14.0
16	29.0
17	73.0
18	229.0
19	509.0
20	1151.0
21	2097.0
22	3811.0
23	6439.0
24	10059.0
25	15063.0
26	21410.0
27	29159.0
28	38141.0
29	48221.0
30	59028.0
31	70510.0
32	85098.0
33	101777.0
34	121232.0
35	145853.0
36	185428.0
37	239759.0
38	272924.0
39	165961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.252517577165264	24.196019414008283	17.244218224093615	20.307244784732838
2	20.02509880035616	25.851148596474594	33.77543593463474	20.3483166685345
3	20.572764969069855	24.626627635035366	35.304332190874035	19.496275205020748
4	14.601208634085847	18.229389241998987	34.660790575265	32.508611548650165
5	10.937313344606036	40.99238784666333	34.40604637243516	13.664252436295477
6	30.838272837999437	38.31914393139825	12.238374563264864	18.604208667337446
7	23.144899389278944	36.25403483536147	20.497517829631118	20.10354794572846
8	27.52376565750113	35.324221573884444	18.120644194332204	19.03136857428223
9	23.38751291578355	14.713155687686195	23.363374717207456	38.5359566793228
10	16.608558469244116	29.629761906228026	32.358733040144536	21.402946584383326
11	31.807926442534463	22.808750343291983	24.192632676963168	21.190690537210386
12	20.47177862808824	29.578283503142277	31.02312710362553	18.926810765143948
13	33.1422392612726	21.571667666267235	27.605786271030098	17.680306801430064
14	19.11437442040614	24.770717902045714	29.806549579983027	26.308358097565122
15	21.154791124532167	30.89597052184076	27.664592341540732	20.28464601208634
16	19.555635468866033	29.35549778261089	28.98517348098686	22.10369326753622
17	20.612112695830373	27.517731107857106	28.398590624772936	23.471565571539585
18	22.284360294633807	24.947628729875085	32.33619584453522	20.431815130955886
19	21.524191770598442	24.018123353583967	34.77440020886931	19.68328466694828
20	22.609240742816112	23.25930952436665	34.695396870162355	19.436052862654883
21	21.221048016542056	24.402671950797483	36.047567029683826	18.328713002976635
22	18.714677872045378	24.897381867533014	35.150820637174554	21.237119623247054
23	20.83508314747331	25.062593058297445	36.356067985975216	17.746255808254034
24	20.181381320728924	25.069058647201754	33.366625984770764	21.382934047298555
25	19.91672321491248	26.175782736508165	34.4365270058412	19.470967042738156
26	19.745723782652764	27.184599336692155	34.09009459464452	18.97958228601056
27	18.04533547785628	27.060275298617842	33.775928550932214	21.11846067259366
28	19.148057059745735	26.497830640980013	33.69883410037796	20.655278198896294
29	19.376384713623672	25.56703214690803	32.66760345865902	22.38897968080927
30	19.792214445726618	26.303247203478858	35.31991118128157	18.584627169512963
31	21.140751560054238	26.024734264296036	34.501059740809936	18.333454434839794
32	20.471470742902323	25.86229404020488	33.36791910255163	20.29831611434117
33	19.77226348567903	25.920792225529592	34.0591829219782	20.24776136681318
34	18.42440371876043	27.003624424408645	35.10937929114978	19.46259256568115
35	17.707647005939723	29.69632668342383	33.52099961699083	19.07502669364562
36	21.28982956707648	26.065251954763045	33.13934514052496	19.505573337635514
37	20.04683549448208	27.820012783392915	32.720990749897474	19.412160972227525
38	19.954285207594666	25.275772761028136	34.523535359382066	20.246406671995132
39	19.14325405084539	25.143997901454572	35.54700729441582	20.16574075328421
40	17.402840671879368	25.983416072345626	34.584065586933846	22.029677668841156
41	19.870602014061735	24.91998064017951	34.49071479856304	20.71870254719572
42	18.446078835849168	27.84889241383217	33.109357123416395	20.595671626902266
43	18.774777060336874	28.037810763912407	31.749612988321303	21.43779918742942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	218.5
2	290.0
3	465.5
4	641.0
5	641.0
6	1420.5
7	2200.0
8	2693.5
9	3187.0
10	5567.5
11	7948.0
12	7948.0
13	16896.0
14	25844.0
15	45335.0
16	64826.0
17	58175.0
18	51524.0
19	51524.0
20	57714.5
21	63905.0
22	49305.5
23	34706.0
24	37377.5
25	40049.0
26	40049.0
27	41500.5
28	42952.0
29	43281.0
30	43610.0
31	46704.0
32	49798.0
33	49798.0
34	54384.0
35	58970.0
36	64579.0
37	70188.0
38	72275.0
39	74362.0
40	74362.0
41	76484.5
42	78607.0
43	82336.5
44	86066.0
45	97755.0
46	109444.0
47	109444.0
48	160218.0
49	210992.0
50	186663.5
51	162335.0
52	139106.5
53	115878.0
54	115878.0
55	95535.5
56	75193.0
57	60209.0
58	45225.0
59	39101.5
60	32978.0
61	32978.0
62	28144.0
63	23310.0
64	20081.5
65	16853.0
66	14162.5
67	11472.0
68	11472.0
69	9663.5
70	7855.0
71	6387.0
72	4919.0
73	4146.0
74	3373.0
75	3373.0
76	2694.0
77	2015.0
78	1539.5
79	1064.0
80	814.5
81	565.0
82	565.0
83	459.0
84	353.0
85	261.5
86	170.0
87	127.5
88	85.0
89	85.0
90	70.0
91	55.0
92	37.0
93	19.0
94	11.0
95	3.0
96	3.0
97	3.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1623982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.33548785139864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06631253739592	30.68171825252825
2	12.561512691878054	10.13349485156874
3	4.58078495617043	5.5430458784844605
4	2.1386729328032197	3.4505766439679744
5	1.1456836040442797	2.3105853546237323
6	0.7350767254121726	1.7789806996625148
7	0.44204285751897204	1.2481010016477825
8	0.3280830954700421	1.0586713369264915
9	0.23336632873768132	0.8471650245951821
>10	1.480946870680066	11.608785661221921
>50	0.15960693757216382	4.493725496070036
>100	0.10740810021497321	8.267206352392309
>500	0.009639170241981633	2.6115059740494537
>1k	0.009333164837474282	6.9966216982692275
>5k	9.180162135220604E-4	2.6730969316565374
>10k+	6.12010809014707E-4	6.296718842335423
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34821	2.1441740117809185	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33038	2.034382154482008	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22874	1.40851314854475	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11297	0.6956357890666276	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9093	0.5599199991132907	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8267	0.5090573663993813	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7706	0.47451264853920794	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6350	0.3910141861178264	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6330	0.38978264537414825	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5568	0.3428609430400091	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4412	0.2716778880554095	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4087	0.25166535097063886	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3774	0.2323917383320751	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3576	0.220199484969661	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3499	0.21545805310649993	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3025	0.18627053748132674	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2937	0.1808517582091427	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2877	0.17715713597810812	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2796	0.17216939596621145	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2779	0.17112258633408498	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2746	0.16909054410701596	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2689	0.16558065298753313	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2433	0.14981693146845224	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2293	0.14119614626270488	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2233	0.1375015240316703	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	2094	0.12894231586310687	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	2086	0.12844969956563557	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2066	0.1272181588219574	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2046	0.12598661807827918	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	2018	0.12426246103712972	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1945	0.11976733732270432	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1917	0.11804318028155485	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1890	0.11638060027758929	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1862	0.11465644323643981	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1835	0.11299386323247425	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1800	0.11083866693103742	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1773	0.10917608692707186	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	1709	0.10523515654730163	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	1703	0.10486569432419818	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1659	0.10215630468810614	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	6.157703718390968E-5	0.0
11	0.0	0.0	0.0	3.0788518591954835E-4	0.0
12	0.0	0.0	0.0	4.926162974712774E-4	0.0
13	0.0	0.0	0.0	6.157703718390967E-4	0.0
14	0.0	0.0	0.0	7.389244462069161E-4	0.0
15	0.0	0.0	0.0	0.001354694818046013	0.0
16	0.0	0.0	0.0	0.002463081487356387	0.0
17	0.0	0.0	0.0	0.0031404288963793933	0.0
18	0.0	0.0	0.0	0.003940930379770219	0.0
19	6.157703718390968E-5	0.0	0.0	0.005357202235000142	0.0
20	6.157703718390968E-5	0.0	0.0	0.008867093354482993	0.0
21	6.157703718390968E-5	0.0	0.0	0.013608525217644038	0.0
22	6.157703718390968E-5	0.0	0.0	0.023460851167069585	0.0
23	6.157703718390968E-5	0.0	0.0	0.027524935621207624	0.0
24	6.157703718390968E-5	0.0	0.0	0.03368263933959859	0.0
25	6.157703718390968E-5	0.0	0.0	0.038608802314311366	0.0
26	6.157703718390968E-5	0.0	0.0	0.048399551226553005	0.0
27	6.157703718390968E-5	0.0	0.0	0.11299386323247425	0.0
28	6.157703718390968E-5	0.0	0.0	0.23664055389776487	0.0
29	6.157703718390968E-5	0.0	0.0	0.39292307427052764	0.0
30	6.157703718390968E-5	0.0	0.0	0.6613989563923738	0.0
31	6.157703718390968E-5	0.0	0.0	0.9576460822841633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6925	0.0	31.576897	1
GGTCTAG	45	0.0038264787	20.555555	1
CGCTTAT	45	0.0038264787	20.555555	1
ATGCGAT	55	5.144352E-4	20.181818	28
TGCGATT	75	9.269639E-6	19.733334	29
GTATCAA	11515	0.0	19.006079	2
GTATTGG	370	0.0	19.000002	1
CGCGCTA	50	0.007036104	18.499998	16
TTATGCG	125	8.58563E-9	17.76	4
GTACACT	65	0.001580314	17.076923	4
CGACCGC	130	1.3942554E-8	17.076923	10
GCGCTAC	65	0.001580314	17.076923	17
TATACCG	120	1.0419535E-7	16.958332	5
CTAGTAC	165	5.4569682E-11	16.818182	3
GTATACT	110	7.812487E-7	16.818182	4
TAGCACC	100	5.880798E-6	16.65	4
TTTTTAC	1205	0.0	16.580914	1
ATTGGAC	480	0.0	16.572916	3
GGACCCT	850	0.0	16.541178	6
GTGCGCG	90	4.4477765E-5	16.444445	11
>>END_MODULE