FastQCFastQC Report
Wed 25 May 2016
SRR2088105_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088105_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3497354
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT335110.9581815280923808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324610.9281588309333285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT261440.7475365662154875No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129680.3707946064367519No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG99690.285044064741516No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG94780.2710048796890449No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA86990.24873089770151952No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA86520.2473870245905905No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA85940.24572862798561426No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG78620.22479851910901785No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA48240.13793284866216002No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46910.13412997368868007No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45830.1310419248380347No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43900.12552346716975174No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39980.11431499356370559No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38970.1114270960274539No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA36920.10556552182021037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78950.029.4781511
GTATTGG11400.018.3377191
GTATCAA133100.017.471452
ATTGGAC12800.016.7656253
TTGGACC18750.016.3786684
GGACCCT17750.016.363386
TGGACCC18600.016.1129045
TATACCG2300.016.0869565
CTAATAC9200.014.8804353
CGGCGTA750.004106635714.812
CTCTAAT6750.014.81
GACCCTC20550.014.3138697
TAATACT11100.014.04
CGAACTA6550.013.83969424
TTACACT5950.013.6806734
TAGGGGT2850.013.6315784
TAGGTCG1805.1686584E-813.36111121
GTCGACG1254.8499132E-513.32000136
GCGAACT7000.013.21428523
TCTAATA9050.012.8784532
Produced by FastQC (version 0.11.5)