##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088105_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3497354
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.428025873274482	31.0	31.0	34.0	30.0	34.0
2	31.633168389588242	31.0	31.0	34.0	30.0	34.0
3	31.709521541142248	31.0	31.0	34.0	30.0	34.0
4	35.443337448825595	37.0	35.0	37.0	33.0	37.0
5	35.31065514100088	37.0	35.0	37.0	33.0	37.0
6	35.33620417035279	37.0	35.0	37.0	33.0	37.0
7	35.19965694064713	37.0	35.0	37.0	32.0	37.0
8	35.21043566078813	37.0	35.0	37.0	32.0	37.0
9	36.75368492866321	39.0	35.0	39.0	32.0	39.0
10	36.582748271979334	39.0	35.0	39.0	32.0	39.0
11	36.64926798945717	39.0	35.0	39.0	32.0	39.0
12	36.51880879087447	38.0	35.0	39.0	32.0	39.0
13	36.552403331204104	38.0	35.0	39.0	32.0	39.0
14	37.51182837081977	39.0	36.0	41.0	32.0	41.0
15	37.522709454061555	39.0	36.0	41.0	32.0	41.0
16	37.39500605314761	39.0	36.0	41.0	32.0	41.0
17	37.40771080079397	39.0	36.0	41.0	32.0	41.0
18	37.39230744156868	39.0	36.0	41.0	32.0	41.0
19	37.4252291875515	39.0	36.0	41.0	32.0	41.0
20	37.335049011338285	39.0	36.0	41.0	31.0	41.0
21	37.24471328896074	39.0	36.0	41.0	31.0	41.0
22	37.15337852559392	39.0	36.0	40.0	31.0	41.0
23	36.958644449489526	39.0	36.0	40.0	31.0	41.0
24	36.81182945735547	39.0	35.0	40.0	30.0	41.0
25	36.6703705143946	39.0	35.0	40.0	30.0	41.0
26	36.779007787029855	39.0	36.0	40.0	30.0	41.0
27	36.70732044854481	39.0	35.0	40.0	30.0	41.0
28	36.616341954517615	39.0	35.0	40.0	30.0	41.0
29	36.515189769179784	39.0	35.0	40.0	30.0	41.0
30	36.31726613891531	38.0	35.0	40.0	30.0	41.0
31	36.1502813269689	38.0	35.0	40.0	30.0	41.0
32	36.0169822671654	38.0	35.0	40.0	29.0	41.0
33	35.862322487228916	38.0	35.0	40.0	29.0	41.0
34	35.72941915516702	38.0	34.0	40.0	27.0	41.0
35	35.54410991852698	38.0	34.0	40.0	27.0	41.0
36	35.44563661556708	38.0	34.0	40.0	27.0	41.0
37	35.28274432613913	38.0	34.0	40.0	26.0	41.0
38	35.19613570716604	38.0	34.0	40.0	25.0	41.0
39	35.12460448670623	38.0	34.0	40.0	25.0	41.0
40	34.95028898990494	38.0	33.0	40.0	24.0	41.0
41	34.832927979266614	38.0	33.0	40.0	24.0	41.0
42	34.648593765458116	38.0	33.0	40.0	23.0	41.0
43	34.31788374868543	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	8.0
14	17.0
15	29.0
16	38.0
17	109.0
18	297.0
19	628.0
20	1346.0
21	2790.0
22	5086.0
23	8970.0
24	14572.0
25	22348.0
26	32907.0
27	45850.0
28	62583.0
29	82645.0
30	104194.0
31	128943.0
32	158358.0
33	192722.0
34	235475.0
35	285568.0
36	360614.0
37	474187.0
38	630655.0
39	646415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46727897719247	21.74340944611269	14.075097916882306	23.714213659812533
2	18.68381067515613	23.302988487868255	35.19003223579883	22.823168601176775
3	19.311971278858245	24.76171986021432	32.65217075537678	23.274138105550655
4	13.839291075481636	16.574273007536554	36.56507176568343	33.021364151298386
5	12.34158738291863	38.62628718739939	35.51316223636498	13.518963193317004
6	32.26991033792976	37.78891127406605	13.705075322658217	16.236103065345972
7	25.86135118149321	33.07191665470524	21.928034737118406	19.138697426683144
8	26.5101845566677	35.11669107559601	19.33264405033062	19.040480317405674
9	24.43301421589007	14.65313491285126	21.845801139947515	39.068049731311156
10	16.005042669400922	27.807250853073494	34.23977098114746	21.94793549637812
11	33.27386933092847	22.468529065116087	23.625575220581045	20.632026383374402
12	20.7990955448033	27.162534876366536	30.998835119350225	21.03953445947994
13	30.711617983195293	21.343278375594807	26.616407718520918	21.328695922688983
14	20.48940427534645	22.63694209965591	27.61939454799257	29.254259077005074
15	22.358931923963087	29.61321616284768	25.757701393682197	22.270150519507034
16	22.09070056963064	28.60173719903676	26.40573416359911	22.901828067733494
17	21.60453302696839	27.06208750958582	27.00613092069033	24.327248542755466
18	22.80978705615731	26.04923607961905	29.605696192035463	21.535280672188172
19	22.672311696213765	24.88415527853343	30.424915521848806	22.018617503404002
20	23.466740856087203	24.787024704962665	30.49419646967393	21.252037969276202
21	22.583129989128924	25.597351597807943	30.753964282712015	21.065554130351117
22	21.30004569168577	25.802192171567416	30.42834668723841	22.4694154495084
23	22.259599685933996	26.079230183733188	31.32788388021344	20.333286250119375
24	22.277842048588735	26.041773294896657	28.884093517556416	22.796291138958193
25	22.129701482892493	26.213846239185397	30.0576378599364	21.59881441798571
26	21.9927693908023	26.910801708949105	29.72164098915923	21.374787911089356
27	20.9764296093561	26.993835911377573	29.39885410513205	22.630880374134275
28	21.366495928064474	26.443305424615293	29.823918310814406	22.366280336505827
29	21.882857726155258	26.16352248013784	29.223121251094398	22.7304985426125
30	21.873393428288928	26.38440375209373	30.504632931067317	21.237569888550027
31	22.556995946078093	26.17387316239649	30.45633927820861	20.81279161331681
32	21.910535793631414	26.080802801203422	29.589884238198366	22.418777166966798
33	21.66778084231679	26.023702490511397	30.30751247943445	22.001004187737358
34	20.941974990235476	26.612804994861825	30.904478071136065	21.54074194376663
35	20.713573747467372	28.38791840917448	29.48969420882187	21.40881363453628
36	22.65341169352602	26.320212366263178	29.373778004743013	21.652597935467785
37	21.733201729078612	26.84549519436694	29.541190282710872	21.880112793843573
38	21.548633624162726	25.55429047216839	30.698979857343577	22.198096046325308
39	21.2208429572757	25.428395295414763	31.32279431821886	22.02796742909068
40	20.166217088690477	25.952648773901643	30.934500768295116	22.946633369112764
41	21.52384345422282	25.023346221171778	30.946910149787527	22.505900174817878
42	20.189863536833847	27.05036436117133	30.452422031055477	22.307350070939343
43	20.731101283999276	26.76223224757917	29.579733707254114	22.926932761167443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	245.0
1	368.5
2	492.0
3	968.5
4	1445.0
5	1445.0
6	2662.0
7	3879.0
8	4358.5
9	4838.0
10	7670.0
11	10502.0
12	10502.0
13	20988.5
14	31475.0
15	49876.5
16	68278.0
17	64766.0
18	61254.0
19	61254.0
20	67157.5
21	73061.0
22	60718.0
23	48375.0
24	55754.0
25	63133.0
26	63133.0
27	70699.5
28	78266.0
29	85757.0
30	93248.0
31	103942.0
32	114636.0
33	114636.0
34	129519.0
35	144402.0
36	158283.0
37	172164.0
38	183893.5
39	195623.0
40	195623.0
41	201703.0
42	207783.0
43	217432.0
44	227081.0
45	236481.5
46	245882.0
47	245882.0
48	303719.5
49	361557.0
50	333644.5
51	305732.0
52	279906.0
53	254080.0
54	254080.0
55	224729.5
56	195379.0
57	166562.0
58	137745.0
59	124636.5
60	111528.0
61	111528.0
62	99945.5
63	88363.0
64	77028.5
65	65694.0
66	55528.0
67	45362.0
68	45362.0
69	38807.0
70	32252.0
71	26897.0
72	21542.0
73	17696.0
74	13850.0
75	13850.0
76	10982.0
77	8114.0
78	6382.0
79	4650.0
80	3583.0
81	2516.0
82	2516.0
83	1946.5
84	1377.0
85	1051.5
86	726.0
87	565.0
88	404.0
89	404.0
90	341.0
91	278.0
92	182.5
93	87.0
94	61.5
95	36.0
96	36.0
97	23.0
98	10.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3497354.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.46703838069033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.58444721804045	32.720824386664866
2	13.768277504031238	12.244690484155047
3	5.215287925209038	6.957252250098635
4	2.506613554734304	4.4584672457571575
5	1.3511032545679613	3.0039780138574583
6	0.8271181906704305	2.2067697797945507
7	0.5272491195586376	1.641164478491939
8	0.36602396605576065	1.3020801397483204
9	0.2702320413273489	1.0814776698055968
>10	1.351172514649808	11.112335703533125
>50	0.1323812320141174	4.11199180851503
>100	0.08679529930864793	7.708940362607229
>500	0.007100969814293117	2.180661030971926
>1k	0.00555166730769053	4.7242153797355995
>5k	3.87325626117944E-4	1.528675905553906
>10k+	2.582170840786293E-4	3.016475360709555
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33511	0.9581815280923808	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32461	0.9281588309333285	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26144	0.7475365662154875	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12968	0.3707946064367519	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9969	0.285044064741516	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9478	0.2710048796890449	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8699	0.24873089770151952	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8652	0.2473870245905905	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8594	0.24572862798561426	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7862	0.22479851910901785	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4824	0.13793284866216002	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4691	0.13412997368868007	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4583	0.1310419248380347	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4390	0.12552346716975174	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3998	0.11431499356370559	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3897	0.1114270960274539	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3692	0.10556552182021037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.577913474014926E-5	0.0
3	2.859304491338309E-5	0.0	0.0	8.577913474014926E-5	0.0
4	2.859304491338309E-5	0.0	0.0	8.577913474014926E-5	0.0
5	5.718608982676618E-5	0.0	0.0	8.577913474014926E-5	0.0
6	8.577913474014926E-5	0.0	0.0	8.577913474014926E-5	0.0
7	1.1437217965353236E-4	0.0	0.0	8.577913474014926E-5	0.0
8	1.1437217965353236E-4	0.0	0.0	8.577913474014926E-5	0.0
9	1.1437217965353236E-4	0.0	0.0	1.7155826948029852E-4	0.0
10	1.1437217965353236E-4	0.0	0.0	2.573374042204478E-4	0.0
11	1.1437217965353236E-4	0.0	0.0	6.004539431810449E-4	0.0
12	1.1437217965353236E-4	0.0	0.0	9.149774372282589E-4	0.0
13	1.1437217965353236E-4	0.0	0.0	0.0010579426617951743	0.0
14	1.4296522456691544E-4	0.0	0.0	0.0012295009312754729	0.0
15	1.4296522456691544E-4	0.0	0.0	0.0016298035600628361	0.0
16	1.4296522456691544E-4	0.0	0.0	0.001972920099023433	0.0
17	1.7155826948029852E-4	0.0	0.0	0.0025447809972910947	0.0
18	1.7155826948029852E-4	0.0	0.0	0.0029736766709918413	0.0
19	1.7155826948029852E-4	0.0	0.0	0.0037170958387398017	0.0
20	1.7155826948029852E-4	0.0	0.0	0.005918760297070299	0.0
21	1.7155826948029852E-4	0.0	0.0	0.00989319354003055	0.0
22	1.7155826948029852E-4	0.0	0.0	0.015697581657447317	0.0
23	1.7155826948029852E-4	0.0	0.0	0.018528293103872242	0.0
24	1.7155826948029852E-4	0.0	0.0	0.023017401155273388	0.0
25	1.7155826948029852E-4	0.0	0.0	0.027134799622800552	0.0
26	2.0015131439368164E-4	0.0	0.0	0.03414009562657941	0.0
27	2.0015131439368164E-4	0.0	0.0	0.07937429267955146	0.0
28	2.0015131439368164E-4	0.0	0.0	0.16795554582121228	0.0
29	2.2874435930706472E-4	0.0	0.0	0.272577497159281	0.0
30	2.573374042204478E-4	0.0	0.0	0.4375879593544148	0.0
31	2.573374042204478E-4	0.0	0.0	0.6610997914423304	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7895	0.0	29.478151	1
GTATTGG	1140	0.0	18.337719	1
GTATCAA	13310	0.0	17.47145	2
ATTGGAC	1280	0.0	16.765625	3
TTGGACC	1875	0.0	16.378668	4
GGACCCT	1775	0.0	16.36338	6
TGGACCC	1860	0.0	16.112904	5
TATACCG	230	0.0	16.086956	5
CTAATAC	920	0.0	14.880435	3
CGGCGTA	75	0.0041066357	14.8	12
CTCTAAT	675	0.0	14.8	1
GACCCTC	2055	0.0	14.313869	7
TAATACT	1110	0.0	14.0	4
CGAACTA	655	0.0	13.839694	24
TTACACT	595	0.0	13.680673	4
TAGGGGT	285	0.0	13.631578	4
TAGGTCG	180	5.1686584E-8	13.361111	21
GTCGACG	125	4.8499132E-5	13.320001	36
GCGAACT	700	0.0	13.214285	23
TCTAATA	905	0.0	12.878453	2
>>END_MODULE