FastQCFastQC Report
Wed 25 May 2016
SRR2088104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331405
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT278660.8364638943628889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT244260.7332041586057534No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT210140.6307849090698969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101180.30371569953217936No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG70480.21156238884194506No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG70310.2110520936361685No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA61010.18313594414368714No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA58230.1747911166609884No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG57920.17386057834457233No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA57250.17184941488651184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG303.6009325E-430.83333433
GGTATCA65550.028.4202921
ACGTTTA1700.018.526
TCGCGTA953.6105466E-617.52631623
TAATACT10950.017.0639274
GTATCAA110200.016.9051722
TTAACGG6150.016.84552835
GTATTGG10650.016.3286381
ATCGCGT1251.6603735E-716.27999922
GTGTCGA803.3849783E-416.187511
CTCTAAT8350.016.1736531
GTATTAG10550.016.1327021
GGTAAAC7550.015.92715335
TAACGGC6400.015.898437536
CTAATAC10050.015.6467663
GGACCCT14200.014.5915496
TATACTG4950.014.5757585
AACGGCC7500.014.55333237
TACCCCG5900.014.4237295
ATTAGAG9750.014.4205123
Produced by FastQC (version 0.11.5)