##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3331405
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67724008338824	31.0	31.0	34.0	30.0	34.0
2	31.88124139814883	31.0	31.0	34.0	30.0	34.0
3	31.96953597656244	31.0	31.0	34.0	30.0	34.0
4	35.65279724320519	37.0	35.0	37.0	33.0	37.0
5	35.541233803755475	37.0	35.0	37.0	33.0	37.0
6	35.561206157762264	37.0	35.0	37.0	33.0	37.0
7	35.44469315499016	37.0	35.0	37.0	33.0	37.0
8	35.45961028454961	37.0	35.0	37.0	33.0	37.0
9	37.04519234377087	39.0	37.0	39.0	33.0	39.0
10	36.87846629275035	39.0	37.0	39.0	33.0	39.0
11	36.92154361298011	39.0	37.0	39.0	33.0	39.0
12	36.80439154050618	39.0	37.0	39.0	32.0	39.0
13	36.82498585431672	39.0	37.0	39.0	32.0	39.0
14	37.881356064483306	40.0	37.0	41.0	33.0	41.0
15	37.87746071102133	40.0	37.0	41.0	33.0	41.0
16	37.773617437687705	40.0	37.0	41.0	32.0	41.0
17	37.739900732573794	39.0	37.0	41.0	32.0	41.0
18	37.715254374655736	39.0	37.0	41.0	32.0	41.0
19	37.74316121876505	40.0	37.0	41.0	32.0	41.0
20	37.65241692319007	39.0	37.0	41.0	32.0	41.0
21	37.55870481073301	39.0	36.0	41.0	32.0	41.0
22	37.43853359168279	39.0	36.0	41.0	32.0	41.0
23	37.25309081303534	39.0	36.0	41.0	31.0	41.0
24	37.114691549061135	39.0	36.0	41.0	31.0	41.0
25	36.95925262764509	39.0	36.0	40.0	31.0	41.0
26	37.161074981877015	39.0	36.0	41.0	31.0	41.0
27	37.106111085262825	39.0	36.0	41.0	31.0	41.0
28	37.0402505849634	39.0	36.0	41.0	31.0	41.0
29	36.975778687970994	39.0	36.0	41.0	31.0	41.0
30	36.774152046959166	39.0	35.0	40.0	30.0	41.0
31	36.6100188959313	39.0	35.0	40.0	30.0	41.0
32	36.465696305312626	39.0	35.0	40.0	30.0	41.0
33	36.32153190620774	38.0	35.0	40.0	30.0	41.0
34	36.209619364802535	38.0	35.0	40.0	30.0	41.0
35	36.031005536702985	38.0	35.0	40.0	29.0	41.0
36	35.952027748052245	38.0	35.0	40.0	28.0	41.0
37	35.80176201932818	38.0	35.0	40.0	27.0	41.0
38	35.734994094083426	38.0	35.0	40.0	27.0	41.0
39	35.671537684550515	38.0	35.0	40.0	27.0	41.0
40	35.52050561249683	38.0	34.0	40.0	26.0	41.0
41	35.40042684693095	38.0	34.0	40.0	25.0	41.0
42	35.24367556631511	38.0	34.0	40.0	25.0	41.0
43	34.92683537426401	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	3.0
14	7.0
15	14.0
16	34.0
17	99.0
18	220.0
19	472.0
20	1056.0
21	2154.0
22	4046.0
23	7085.0
24	11626.0
25	17423.0
26	25383.0
27	36055.0
28	49127.0
29	65362.0
30	84242.0
31	105563.0
32	131829.0
33	164112.0
34	204136.0
35	254362.0
36	330848.0
37	453280.0
38	639972.0
39	742893.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54118307440854	21.633995266261532	13.674290577098851	24.150531082231073
2	18.378041697121787	22.958841689917616	35.61065076146551	23.05246585149509
3	18.694694880988653	24.912521893915628	32.496139016421	23.89664420867472
4	13.359288348309498	16.611279625263215	37.465964060208826	32.563467966218454
5	12.174713071511869	38.74227240458605	36.00498888607059	13.078025637831486
6	31.84377162188326	38.776402148642994	14.1623128980115	15.217513331462252
7	26.188530064642396	32.90086314933189	22.283480993754885	18.627125792270828
8	26.225001163172895	35.617314616505645	19.44302779157743	18.71465642874403
9	24.7428937640425	14.631844522056008	21.50447033608943	39.12079137781206
10	15.768091841130094	27.862328356954496	34.535338693434156	21.834241108481255
11	33.58414842986668	22.720443776724835	23.482554657869578	20.212853135538907
12	20.698384015152765	27.106100879358706	31.061459054062773	21.134056051425752
13	29.991400024914412	21.61517437837789	26.34432018922947	22.049105407478226
14	20.58179656931535	22.367829789533246	27.5319872546268	29.518386386524604
15	22.668363648370583	29.71382945033702	25.305809410744118	22.311997490548283
16	22.270903717800746	28.630412693743327	26.06086621110312	23.0378173773528
17	21.785192733996617	27.295030174956214	26.968321173799044	23.951455917248126
18	22.39169359474456	26.6622641197933	29.23934496105997	21.706697324402167
19	22.7901440983609	25.434073611584303	29.872111016222885	21.90367127383191
20	23.34618576846706	25.449832728233286	29.728357855019127	21.475623648280532
21	22.351860551328944	26.148667003861735	30.06326159683377	21.436210847975552
22	21.620697573546295	26.442086747183247	29.668353142292816	22.26886253697764
23	22.36834008473902	26.514338544848194	30.339841598364654	20.777479772048128
24	22.48171567251655	26.699305548259666	28.207738176535123	22.611240602688657
25	22.405381513205388	26.529407262101124	29.38279794861327	21.68241327608021
26	22.030794814800363	27.192791029610632	29.212779592994547	21.563634562594462
27	21.53412749275456	27.156500035270405	28.688256156186355	22.621116315788683
28	21.642940441045145	26.688079053732583	29.366528536758512	22.302451968463757
29	22.202554177591736	26.634588109221184	28.772995177710303	22.389862535476773
30	22.071408309707166	26.66400512696595	29.734721536408813	21.52986502691807
31	22.48132544677096	26.581577442550515	29.7757852917913	21.161311818887228
32	21.809596851778753	26.406336065413843	29.20596565112918	22.578101431678228
33	21.747701045054562	26.337716368919416	29.90401947526644	22.010563110759573
34	21.311638783035985	26.6188289925722	30.45699937413794	21.61253285025387
35	21.180132706770866	28.079023715219254	29.059240770785898	21.681602807223978
36	22.714200164795333	26.4837208325016	29.027842606948116	21.774236395754944
37	21.763610248528774	26.723709666041806	29.326215215502167	22.186464869927253
38	21.7002135735523	25.72716316389031	30.186993175552058	22.38563008700533
39	21.27390695517357	25.86269156707155	30.797876571596667	22.065524906158213
40	20.497958068742765	26.28794757767368	30.485816044581792	22.728278309001755
41	21.492043146960516	25.171721841085066	30.70269150703682	22.633543504917593
42	20.37359612535852	27.028445955985536	30.100903372601053	22.497054546054894
43	21.02194119298014	26.29758315185335	29.608138308011185	23.07233734715533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	220.0
1	315.5
2	411.0
3	870.0
4	1329.0
5	1329.0
6	2236.0
7	3143.0
8	3589.5
9	4036.0
10	6325.0
11	8614.0
12	8614.0
13	16346.5
14	24079.0
15	39126.0
16	54173.0
17	51449.0
18	48725.0
19	48725.0
20	54195.5
21	59666.0
22	51755.5
23	43845.0
24	50997.0
25	58149.0
26	58149.0
27	67139.5
28	76130.0
29	84580.5
30	93031.0
31	105242.0
32	117453.0
33	117453.0
34	133641.0
35	149829.0
36	165841.5
37	181854.0
38	194224.5
39	206595.0
40	206595.0
41	211734.5
42	216874.0
43	226183.0
44	235492.0
45	237215.5
46	238939.0
47	238939.0
48	275972.5
49	313006.0
50	289965.0
51	266924.0
52	246980.0
53	227036.0
54	227036.0
55	204238.5
56	181441.0
57	157802.5
58	134164.0
59	122311.5
60	110459.0
61	110459.0
62	98465.5
63	86472.0
64	75030.5
65	63589.0
66	53819.0
67	44049.0
68	44049.0
69	37617.5
70	31186.0
71	25781.0
72	20376.0
73	16578.0
74	12780.0
75	12780.0
76	10134.0
77	7488.0
78	5980.0
79	4472.0
80	3456.5
81	2441.0
82	2441.0
83	1912.5
84	1384.0
85	1051.5
86	719.0
87	572.5
88	426.0
89	426.0
90	338.5
91	251.0
92	166.5
93	82.0
94	67.0
95	52.0
96	52.0
97	32.0
98	12.0
99	10.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3331405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.121004381578786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.88169211102989	33.78737157841813
2	13.185292364640128	11.898672691146489
3	5.000769554126638	6.769192348890414
4	2.2821997883562837	4.119005866002482
5	1.2547332478326458	2.8307412186584693
6	0.7373914643015961	1.9963106095014667
7	0.4703165396193259	1.4854808251424803
8	0.3418981028498434	1.2341428637393006
9	0.25961922368439266	1.0542852116465007
>10	1.3373710222287707	11.280206345165022
>50	0.13719716103362767	4.30913913330228
>100	0.09566372440577514	8.489468354858795
>500	0.009659982791054624	3.0395639503412246
>1k	0.005529507390367504	4.070857995727194
>5k	3.997234258096991E-4	1.1278463503761074
>10k+	2.6648228387313275E-4	2.507714657083592
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27866	0.8364638943628889	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24426	0.7332041586057534	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21014	0.6307849090698969	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10118	0.30371569953217936	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7048	0.21156238884194506	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7031	0.2110520936361685	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6101	0.18313594414368714	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5823	0.1747911166609884	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5792	0.17386057834457233	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5725	0.17184941488651184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.0017365045678924E-5	0.0
3	0.0	0.0	0.0	3.0017365045678924E-5	0.0
4	0.0	0.0	0.0	3.0017365045678924E-5	0.0
5	3.0017365045678924E-5	0.0	0.0	3.0017365045678924E-5	0.0
6	3.0017365045678924E-5	0.0	0.0	3.0017365045678924E-5	0.0
7	3.0017365045678924E-5	0.0	0.0	3.0017365045678924E-5	0.0
8	3.0017365045678924E-5	0.0	0.0	3.0017365045678924E-5	0.0
9	3.0017365045678924E-5	0.0	0.0	1.200694601827157E-4	0.0
10	3.0017365045678924E-5	0.0	0.0	1.8010419027407357E-4	0.0
11	3.0017365045678924E-5	0.0	0.0	2.1012155531975247E-4	0.0
12	6.003473009135785E-5	0.0	0.0	3.90225745593826E-4	0.0
13	6.003473009135785E-5	0.0	0.0	4.802778407308628E-4	0.0
14	1.5008682522839464E-4	0.0	0.0	5.102952057765418E-4	0.0
15	1.5008682522839464E-4	0.0	0.0	5.403125708222207E-4	0.0
16	1.8010419027407357E-4	0.0	0.0	9.905730465074045E-4	0.0
17	1.8010419027407357E-4	0.0	0.0	0.0016809724425580197	0.0
18	2.1012155531975247E-4	0.0	0.0	0.0019511287279691302	0.0
19	2.1012155531975247E-4	0.0	0.0	0.0024914412987913507	0.0
20	2.1012155531975247E-4	0.0	0.0	0.004172413741349371	0.0
21	2.1012155531975247E-4	0.0	0.0	0.007294219706099979	0.0
22	2.1012155531975247E-4	0.0	0.0	0.011616720272677744	3.0017365045678924E-5
23	2.1012155531975247E-4	0.0	0.0	0.014168196301560453	3.0017365045678924E-5
24	2.1012155531975247E-4	0.0	0.0	0.017380054361448096	3.0017365045678924E-5
25	2.1012155531975247E-4	0.0	0.0	0.020291738770878955	3.0017365045678924E-5
26	2.1012155531975247E-4	0.0	0.0	0.025544777653872766	9.005209513703678E-5
27	2.1012155531975247E-4	0.0	0.0	0.060515007932088714	9.005209513703678E-5
28	2.1012155531975247E-4	0.0	0.0	0.13841007022562551	9.005209513703678E-5
29	2.1012155531975247E-4	0.0	0.0	0.23209426653318946	9.005209513703678E-5
30	2.401389203654314E-4	0.0	0.0	0.3729057259624693	9.005209513703678E-5
31	2.401389203654314E-4	0.0	0.0	0.5948841404752649	1.200694601827157E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	30	3.6009325E-4	30.833334	33
GGTATCA	6555	0.0	28.420292	1
ACGTTTA	170	0.0	18.5	26
TCGCGTA	95	3.6105466E-6	17.526316	23
TAATACT	1095	0.0	17.063927	4
GTATCAA	11020	0.0	16.905172	2
TTAACGG	615	0.0	16.845528	35
GTATTGG	1065	0.0	16.328638	1
ATCGCGT	125	1.6603735E-7	16.279999	22
GTGTCGA	80	3.3849783E-4	16.1875	11
CTCTAAT	835	0.0	16.173653	1
GTATTAG	1055	0.0	16.132702	1
GGTAAAC	755	0.0	15.927153	35
TAACGGC	640	0.0	15.8984375	36
CTAATAC	1005	0.0	15.646766	3
GGACCCT	1420	0.0	14.591549	6
TATACTG	495	0.0	14.575758	5
AACGGCC	750	0.0	14.553332	37
TACCCCG	590	0.0	14.423729	5
ATTAGAG	975	0.0	14.420512	3
>>END_MODULE