FastQCFastQC Report
Wed 25 May 2016
SRR2088103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3047326
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT292530.9599563683045398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT274240.8999365345223976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT224040.735201944261953No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107420.3525057706330074No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG80770.2650520489110781No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77910.25566677145799305No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA72450.23774942359301235No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA68870.2260014189489408No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68560.2249841336305994No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG68510.22482005535344757No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38980.1279154248675724No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38800.1273247430698258No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35700.11715188988641188No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC33550.11009652396888289No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA32660.10717593063558019No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32490.10661806449326393No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31870.10458349385658115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.030.6254671
GTATTGG10800.020.726851
TTGGACC17000.017.5205884
GTATCAA117300.017.3802222
TATACCG2050.017.1463435
ATTGGAC13550.016.7933583
GGACCCT16550.016.655596
GTATTAG5750.016.0869561
TATACTG4700.015.7446815
TGGACCC17850.015.5462195
GCGCATA1455.354559E-815.31034526
TAATACT9950.014.6884424
TACACCG3200.014.4531255
GACCCTC17550.014.4415957
TCTATAC3750.014.3066663
TATTCCG800.006301769513.8755
CTAATAC8100.013.7037043
ATAGTCG1356.5750246E-613.70370330
ATTAGAG5850.013.5982913
GTACTAG3050.013.3442621
Produced by FastQC (version 0.11.5)