FastQCFastQC Report
Wed 25 May 2016
SRR2088102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396828
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT306820.9032544479732267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT296420.8726376490066615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237550.6993289033180367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115740.3407296454221409No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG86230.2538544783545119No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG83430.2456114940173597No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA81310.23937037730494448No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA76640.22562225699976568No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75930.22353207168570205No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG69230.2038077877360879No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44550.13115176865004646No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA42530.12520504423538667No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA40370.11884617060386926No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC36750.10818916942512251No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA35280.1038616026481176No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35270.10383216341834205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74050.029.4550971
GTATTGG11950.019.81591
GTATTAG8050.017.465841
GTATCAA125100.017.4056762
TGGACCC18550.016.5552585
ATTGGAC15700.015.9076433
TTGGACC20300.015.7660084
GGACCCT19000.015.5789476
GACCCTC20450.014.7457217
ACGCGAT908.2798896E-414.3888886
TAATACT11550.014.2554114
GTACCGT1051.6571351E-414.0952396
TAGACAG4750.014.0210525
CGCGATA800.006301966513.87514
GTCGTCA1601.7864659E-713.87524
CTAATAC9350.013.6524063
CTAGTAC3950.013.5822783
TTAACGG5600.013.21428535
CCCTCGC21600.013.1041679
TAACGGC5800.013.07758636
Produced by FastQC (version 0.11.5)