##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088102_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3396828
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51260793893597	31.0	31.0	34.0	30.0	34.0
2	31.71326013563242	31.0	31.0	34.0	30.0	34.0
3	31.78748879837307	31.0	31.0	34.0	30.0	34.0
4	35.510654940432666	37.0	35.0	37.0	33.0	37.0
5	35.38522586365868	37.0	35.0	37.0	33.0	37.0
6	35.41089275053079	37.0	35.0	37.0	33.0	37.0
7	35.2870657566412	37.0	35.0	37.0	32.0	37.0
8	35.28822271837137	37.0	35.0	37.0	32.0	37.0
9	36.85049493233099	39.0	37.0	39.0	33.0	39.0
10	36.67356575016456	39.0	35.0	39.0	32.0	39.0
11	36.73467275940966	39.0	35.0	39.0	32.0	39.0
12	36.615015832417775	39.0	35.0	39.0	32.0	39.0
13	36.652466359792136	39.0	35.0	39.0	32.0	39.0
14	37.641297116015295	39.0	36.0	41.0	32.0	41.0
15	37.65088547315319	39.0	36.0	41.0	32.0	41.0
16	37.52875241254488	39.0	36.0	41.0	32.0	41.0
17	37.52145501626812	39.0	36.0	41.0	32.0	41.0
18	37.509619857113755	39.0	36.0	41.0	32.0	41.0
19	37.53993254883674	39.0	36.0	41.0	32.0	41.0
20	37.44860734779624	39.0	36.0	41.0	32.0	41.0
21	37.35908912667936	39.0	36.0	41.0	31.0	41.0
22	37.26762438368972	39.0	36.0	40.0	31.0	41.0
23	37.08498075263157	39.0	36.0	40.0	31.0	41.0
24	36.95226546648815	39.0	36.0	40.0	31.0	41.0
25	36.80962356645671	39.0	35.0	40.0	30.0	41.0
26	36.93421804106655	39.0	36.0	40.0	31.0	41.0
27	36.86491779978262	39.0	36.0	40.0	30.0	41.0
28	36.78221387718189	39.0	35.0	40.0	30.0	41.0
29	36.67968057258124	39.0	35.0	40.0	30.0	41.0
30	36.48615119752899	39.0	35.0	40.0	30.0	41.0
31	36.335325486012245	38.0	35.0	40.0	30.0	41.0
32	36.20364498879543	38.0	35.0	40.0	30.0	41.0
33	36.061584219159755	38.0	35.0	40.0	29.0	41.0
34	35.93767244028841	38.0	35.0	40.0	29.0	41.0
35	35.748951374635396	38.0	35.0	40.0	27.0	41.0
36	35.65093758058989	38.0	34.0	40.0	27.0	41.0
37	35.50368078689883	38.0	34.0	40.0	27.0	41.0
38	35.41785218444973	38.0	34.0	40.0	26.0	41.0
39	35.359154187377165	38.0	34.0	40.0	26.0	41.0
40	35.19405074381158	38.0	34.0	40.0	25.0	41.0
41	35.08943431931202	38.0	34.0	40.0	24.0	41.0
42	34.909703994432455	38.0	33.0	40.0	24.0	41.0
43	34.58023632636095	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	2.0
14	7.0
15	14.0
16	38.0
17	94.0
18	216.0
19	530.0
20	1118.0
21	2229.0
22	4331.0
23	7628.0
24	12664.0
25	19540.0
26	28772.0
27	41018.0
28	56130.0
29	73968.0
30	95395.0
31	119355.0
32	148249.0
33	181781.0
34	223263.0
35	274293.0
36	347485.0
37	461563.0
38	625647.0
39	671496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.30642705488768	21.94691635843793	13.957580425031823	23.789076161642566
2	18.465226970573724	23.034901973252694	35.642870348454494	22.857000707719084
3	19.001020952488616	24.983190199798162	32.677191780096024	23.3385970676172
4	13.439126149454728	16.552030305920702	37.23550324008163	32.77334030454294
5	12.09808091548939	38.66636756409215	36.051751810807026	13.183799709611439
6	31.86987978196129	38.31833698968567	13.910889806607813	15.900893421745227
7	25.82827272973492	32.99136724026062	22.261651163968267	18.918708866036198
8	26.083039824212474	35.539332577333916	19.4294500634121	18.948177535041516
9	24.369352819748304	14.600444885640368	21.92775141985405	39.102450874757274
10	15.79208602849482	27.75312732937906	34.67832342408859	21.776463218037534
11	33.102176501135766	22.595845300380237	23.724928079961657	20.57705011852234
12	20.541752482021465	27.235055763789042	31.230194758168505	20.99299699602099
13	30.270711381323988	21.54436433048715	26.667644049095212	21.517280239093648
14	20.328111991540343	22.666499451841542	27.68986242459141	29.315526132026704
15	22.283318437083068	29.66835530088659	25.851294207419397	22.19703205461095
16	22.021427048999833	28.62367479307165	26.5124993081781	22.842398849750413
17	21.456017201930745	27.14859274593827	27.243092673517765	24.152297378613223
18	22.52324815975375	26.26323734966857	29.716723955407808	21.496790535169872
19	22.534670580906656	25.141808769828796	30.36429869278044	21.959221956484107
20	23.20093922918676	25.049016317576278	30.572787318050842	21.17725713518612
21	22.28184647559429	25.823032546834874	30.82552310567388	21.06959787189696
22	21.174578165276547	26.094197292297398	30.36250289976413	22.368721642661917
23	22.150665267714466	26.322675154585397	31.25351062815073	20.2731489495494
24	22.18696383802771	26.329681691271972	28.766042908266183	22.71731156243413
25	21.967111670063954	26.44405309894996	30.03293072242692	21.555904508559163
26	21.87637996389573	27.05259141764022	29.787701938396644	21.283326680067404
27	21.002682502617148	27.079911022871926	29.32506444247398	22.592342032036946
28	21.281089298604464	26.562722634175177	30.05765967543838	22.09852839178198
29	21.875879496989544	26.406047053309734	29.22959302031189	22.48848042938883
30	21.80225198332091	26.53372499284627	30.45820983576443	21.205813188068397
31	22.348467452576344	26.471667096479422	30.410253330460062	20.76961212048417
32	21.660443213492115	26.152693041861408	29.705890318850408	22.480973425796066
33	21.52737789490666	26.19381964585784	30.40507202601957	21.87373043321593
34	20.91236294566578	26.74218417888689	30.95917720885485	21.386275666592482
35	20.724334585089384	28.484515553922655	29.39889803075104	21.392251830236916
36	22.63658919438959	26.496543245639753	29.346231248682596	21.52063631128806
37	21.629590900687347	26.934392910091415	29.590753491198257	21.845262698022978
38	21.534826020039873	25.68319620540104	30.62083214104453	22.16114563351456
39	21.180995917367614	25.769040999426522	31.14249529266716	21.907467790538703
40	20.102371977621473	26.45365028785679	30.794170325962927	22.649807408558807
41	21.449275618312143	25.201629284732697	30.968715519302126	22.380379577653034
42	20.206145262580264	27.273621154795002	30.327499655561013	22.19273392706372
43	20.828961607711665	26.746040717987484	29.55407221089793	22.870925463402916
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	337.0
2	470.0
3	953.5
4	1437.0
5	1437.0
6	2490.5
7	3544.0
8	4060.5
9	4577.0
10	7210.5
11	9844.0
12	9844.0
13	19059.5
14	28275.0
15	45069.5
16	61864.0
17	59068.5
18	56273.0
19	56273.0
20	62484.5
21	68696.0
22	58265.5
23	47835.0
24	54876.0
25	61917.0
26	61917.0
27	69778.0
28	77639.0
29	85317.0
30	92995.0
31	104553.5
32	116112.0
33	116112.0
34	132208.5
35	148305.0
36	162919.5
37	177534.0
38	189575.5
39	201617.0
40	201617.0
41	206373.0
42	211129.0
43	221269.0
44	231409.0
45	237068.0
46	242727.0
47	242727.0
48	293485.5
49	344244.0
50	316647.0
51	289050.0
52	265295.0
53	241540.0
54	241540.0
55	213073.5
56	184607.0
57	157256.0
58	129905.0
59	117647.5
60	105390.0
61	105390.0
62	94025.0
63	82660.0
64	71438.5
65	60217.0
66	50456.0
67	40695.0
68	40695.0
69	34674.5
70	28654.0
71	23729.0
72	18804.0
73	15183.5
74	11563.0
75	11563.0
76	9191.0
77	6819.0
78	5319.0
79	3819.0
80	2986.0
81	2153.0
82	2153.0
83	1602.0
84	1051.0
85	808.5
86	566.0
87	439.0
88	312.0
89	312.0
90	273.0
91	234.0
92	159.0
93	84.0
94	61.0
95	38.0
96	38.0
97	27.0
98	16.0
99	10.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3396828.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.12393084588438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.04030350131168	32.66949231500168
2	13.858017284998	12.229403926886445
3	5.201162864983714	6.884872517181699
4	2.326771839893085	4.106652790303745
5	1.2613639626620878	2.7828168129996325
6	0.7392175741827073	1.9570311073980111
7	0.49380605373926695	1.5252064916529338
8	0.31023000085866514	1.0950853683365114
9	0.2337403846794303	0.9282190112537037
>10	1.2814506339454819	10.717160144180662
>50	0.13898216009176353	4.297162574438667
>100	0.0982468706505305	8.569594417326995
>500	0.009623155970387326	2.9486022930868545
>1k	0.006415437313104931	5.074136535773189
>5k	4.009648320690582E-4	1.394052527598916
>10k+	2.673098880460388E-4	2.820511166580348
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30682	0.9032544479732267	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29642	0.8726376490066615	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23755	0.6993289033180367	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11574	0.3407296454221409	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8623	0.2538544783545119	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8343	0.2456114940173597	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8131	0.23937037730494448	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7664	0.22562225699976568	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7593	0.22353207168570205	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6923	0.2038077877360879	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4455	0.13115176865004646	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4253	0.12520504423538667	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4037	0.11884617060386926	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3675	0.10818916942512251	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3528	0.1038616026481176	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3527	0.10383216341834205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.9439229775543536E-5	0.0
6	5.887845955108707E-5	0.0	0.0	2.9439229775543536E-5	0.0
7	5.887845955108707E-5	0.0	0.0	2.9439229775543536E-5	0.0
8	8.831768932663061E-5	0.0	0.0	2.9439229775543536E-5	0.0
9	1.1775691910217414E-4	0.0	0.0	2.6495306797989185E-4	0.0
10	1.1775691910217414E-4	0.0	0.0	4.121492168576095E-4	0.0
11	1.1775691910217414E-4	0.0	0.0	6.182238252864143E-4	0.0
12	1.1775691910217414E-4	0.0	0.0	7.948592039396755E-4	0.0
13	1.1775691910217414E-4	0.0	0.0	9.714945825929367E-4	0.0
14	1.1775691910217414E-4	0.0	0.0	0.0011186907314706543	0.0
15	1.1775691910217414E-4	0.0	0.0	0.0013836437994505463	0.0
16	1.1775691910217414E-4	0.0	0.0	0.0019135499354103298	0.0
17	1.1775691910217414E-4	0.0	0.0	0.0022373814629413086	0.0
18	1.1775691910217414E-4	0.0	0.0	0.0025612129904722877	0.0
19	1.1775691910217414E-4	0.0	0.0	0.0030322406668809843	0.0
20	1.1775691910217414E-4	0.0	0.0	0.004798594453413596	0.0
21	1.1775691910217414E-4	0.0	0.0	0.008360741256254365	0.0
22	1.1775691910217414E-4	0.0	0.0	0.014219147981587528	0.0
23	1.1775691910217414E-4	0.0	0.0	0.017133631729366338	0.0
24	1.1775691910217414E-4	0.0	0.0	0.021490637736146782	0.0
25	1.1775691910217414E-4	0.0	0.0	0.024022411496843525	0.0
26	1.1775691910217414E-4	0.0	0.0	0.02979250053285006	0.0
27	1.1775691910217414E-4	0.0	0.0	0.06517845472305339	0.0
28	1.1775691910217414E-4	0.0	0.0	0.13865877224281006	0.0
29	1.1775691910217414E-4	0.0	0.0	0.22927272149193306	0.0
30	1.1775691910217414E-4	0.0	0.0	0.3719646682139926	0.0
31	1.1775691910217414E-4	0.0	0.0	0.572386944525893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7405	0.0	29.455097	1
GTATTGG	1195	0.0	19.8159	1
GTATTAG	805	0.0	17.46584	1
GTATCAA	12510	0.0	17.405676	2
TGGACCC	1855	0.0	16.555258	5
ATTGGAC	1570	0.0	15.907643	3
TTGGACC	2030	0.0	15.766008	4
GGACCCT	1900	0.0	15.578947	6
GACCCTC	2045	0.0	14.745721	7
ACGCGAT	90	8.2798896E-4	14.388888	6
TAATACT	1155	0.0	14.255411	4
GTACCGT	105	1.6571351E-4	14.095239	6
TAGACAG	475	0.0	14.021052	5
CGCGATA	80	0.0063019665	13.875	14
GTCGTCA	160	1.7864659E-7	13.875	24
CTAATAC	935	0.0	13.652406	3
CTAGTAC	395	0.0	13.582278	3
TTAACGG	560	0.0	13.214285	35
CCCTCGC	2160	0.0	13.104167	9
TAACGGC	580	0.0	13.077586	36
>>END_MODULE