FastQCFastQC Report
Wed 25 May 2016
SRR2088101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468656
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146840.9998256909718818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146790.9994852436513383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115800.7884759943785338No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54030.3678873745792071No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG45460.3095347038380669No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA44420.302453399570764No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG44100.30027453671928617No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA39930.27188123018596594No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA39780.2708598882243357No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG37140.2528842696996437No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA22330.1520437733546862No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA21640.14734560033118715No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21430.14591572158490485No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19320.131548844657973No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18610.1267144927062566No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC17500.11915656219019294No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17390.11840757808499744No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16760.1141179418461505No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG16620.11316468934862896No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16600.11302851042041158No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG16320.11112200542536851No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT15430.10506204311969583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37000.029.81
ATAACGC400.001931441123.1250023
GTATTGG5450.020.0275231
TTAACGG1500.019.73333435
GTATTAG2950.018.1864411
GTATCAA61350.017.8818242
ATTGGAC6150.016.8455283
CCGAAAT1005.8800306E-616.6524
TGGACCC8750.016.4914285
GTCGGTT1801.0913936E-1116.44444512
GGACCCT8650.016.2543356
TATGCCG1151.2426935E-616.08695610
CGACGAA1504.6729838E-916.03333310
TTGGACC8600.015.9186044
GTAAGAC700.002592953215.8571433
CGAACTA2003.6379788E-1215.72499924
TCCGACG957.0618116E-515.57894823
ACGACAT957.0618116E-515.57894827
GACCCTC9300.015.1182797
TAAGCCG1353.974037E-715.07407310
Produced by FastQC (version 0.11.5)