##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1468656
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.591278012005535	31.0	31.0	34.0	30.0	34.0
2	31.79456319246985	31.0	31.0	34.0	30.0	34.0
3	31.867215331568456	31.0	31.0	34.0	30.0	34.0
4	35.57223747426218	37.0	35.0	37.0	33.0	37.0
5	35.46302333562114	37.0	35.0	37.0	33.0	37.0
6	35.49441938752165	37.0	35.0	37.0	33.0	37.0
7	35.359577736379386	37.0	35.0	37.0	33.0	37.0
8	35.3701145809502	37.0	35.0	37.0	33.0	37.0
9	36.941919687115295	39.0	37.0	39.0	33.0	39.0
10	36.779217189049035	39.0	37.0	39.0	32.0	39.0
11	36.837537176847405	39.0	37.0	39.0	32.0	39.0
12	36.70998586462725	39.0	35.0	39.0	32.0	39.0
13	36.74104895904827	39.0	35.0	39.0	32.0	39.0
14	37.730962866729854	39.0	37.0	41.0	32.0	41.0
15	37.739582992886014	39.0	37.0	41.0	32.0	41.0
16	37.6151263468096	39.0	36.0	41.0	32.0	41.0
17	37.62898051007179	39.0	36.0	41.0	32.0	41.0
18	37.61089731019381	39.0	36.0	41.0	32.0	41.0
19	37.63934032203593	39.0	37.0	41.0	32.0	41.0
20	37.55027930298177	39.0	36.0	41.0	32.0	41.0
21	37.46952043228639	39.0	36.0	41.0	32.0	41.0
22	37.36241774792736	39.0	36.0	41.0	31.0	41.0
23	37.16815510235208	39.0	36.0	41.0	31.0	41.0
24	37.02416358902289	39.0	36.0	40.0	31.0	41.0
25	36.876045173274065	39.0	36.0	40.0	30.0	41.0
26	37.01039385669619	39.0	36.0	40.0	31.0	41.0
27	36.94430009478054	39.0	36.0	41.0	31.0	41.0
28	36.854650101861836	39.0	35.0	41.0	30.0	41.0
29	36.74529501803009	39.0	35.0	40.0	30.0	41.0
30	36.54821006416751	39.0	35.0	40.0	30.0	41.0
31	36.37539423799719	38.0	35.0	40.0	30.0	41.0
32	36.23149056007669	38.0	35.0	40.0	30.0	41.0
33	36.060541066117594	38.0	35.0	40.0	29.0	41.0
34	35.92506414027519	38.0	35.0	40.0	29.0	41.0
35	35.72517049601813	38.0	35.0	40.0	27.0	41.0
36	35.617979976250396	38.0	34.0	40.0	27.0	41.0
37	35.46294299005349	38.0	34.0	40.0	27.0	41.0
38	35.363444537046114	38.0	34.0	40.0	26.0	41.0
39	35.280168398862635	38.0	34.0	40.0	25.0	41.0
40	35.11213449575666	38.0	34.0	40.0	25.0	41.0
41	34.982485347147325	38.0	34.0	40.0	24.0	41.0
42	34.78008124434857	38.0	33.0	40.0	23.0	41.0
43	34.44496532884487	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	4.0
15	4.0
16	24.0
17	43.0
18	94.0
19	201.0
20	492.0
21	1004.0
22	1864.0
23	3207.0
24	5413.0
25	8272.0
26	12373.0
27	17371.0
28	23817.0
29	31266.0
30	40232.0
31	50043.0
32	62108.0
33	77714.0
34	94860.0
35	117470.0
36	150399.0
37	204361.0
38	271052.0
39	294964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68399952065017	21.692758549313112	14.04692453508514	23.576317394951573
2	18.509780370624572	23.22238836051465	35.63482530967088	22.633005959189898
3	19.25379394494014	24.74017060496127	33.00391650597553	23.002118944123062
4	13.613535096033381	16.424472442832087	36.79813380396771	33.16385865716682
5	12.294982623568758	38.527129566079466	35.623113921844194	13.554773888507587
6	31.997622315913326	37.76561699948797	13.695855258140776	16.54090542645793
7	25.668638537547256	33.22309649094138	21.889809458443636	19.21845551306773
8	26.36580656055604	35.26864017169439	19.32447080868495	19.041082459064615
9	24.455352376594654	14.655576254752646	22.127305509254718	38.761765859397975
10	15.975762874355873	27.647726901330195	34.233339869921885	22.14317035439204
11	32.853574969223565	22.52542460589818	23.807140678280007	20.81385974659825
12	20.695928794762015	27.241232800601367	31.17748472072425	20.885353683912367
13	31.11988103408831	21.340463662014795	26.59894488566417	20.940710418232726
14	20.391228442875665	22.902163610811517	27.69082753211099	29.01578041420183
15	22.26682082121341	29.595289843230816	25.773292043882297	22.364597291673476
16	21.71781547210511	28.80742665402926	26.54209018313342	22.932667690732206
17	21.51273000620976	26.917126951444043	27.038258108093387	24.531884934252812
18	23.04290453312416	25.880124413068817	29.655753287359328	21.421217766447693
19	22.672021222124172	24.872808881044982	30.62296412502315	21.832205771807693
20	23.639708686036755	24.563614624527457	30.64250580122234	21.154170888213443
21	22.553068928326304	25.335544879127582	31.054515148543977	21.056871044002136
22	21.08397065071739	25.620499286422415	30.735856456515343	22.559673606344848
23	22.35819688204726	25.84070061334989	31.613121112091598	20.187981392511247
24	22.24857284483228	25.90130023640662	29.148214421893215	22.701912496867884
25	22.04811746249632	26.29288274449565	30.156074669629916	21.50292512337811
26	21.929573705483108	26.95021843100086	29.924298133803966	21.195909729712064
27	20.751625976402917	26.987327188940093	29.61884879781242	22.642198036844572
28	21.322215685633665	26.37295595428746	29.91755727685721	22.387271083221666
29	21.67948110381192	26.25005447157129	29.165032519528054	22.905431905088733
30	21.77603196391803	26.38432689479361	30.71495299103398	21.124688150254382
31	22.55688193831639	26.063080803128848	30.655783246723534	20.724254011831224
32	21.827575688248302	26.027810460720552	29.774841760085412	22.369772090945737
33	21.697252453944284	25.92036528635705	30.449608349402446	21.932773910296216
34	20.76156703816278	26.654914425161508	30.9927580045974	21.59076053207831
35	20.479404298896405	28.578714144088202	29.640977873647746	21.30090368336765
36	22.586637034131886	26.1356641718687	29.657864060746697	21.619834733252716
37	21.674851020252532	26.970304822912922	29.635803074375485	21.719041082459064
38	21.619902822716824	25.387156692921963	30.93202220261246	22.060918281748755
39	21.1899178568705	25.152656578531662	31.661873168393416	21.99555239620442
40	19.848555413929468	25.860855094726066	31.31536588554433	22.97522360580013
41	21.49414158250809	24.950090422808337	31.18075301500147	22.375014979682103
42	20.088979311697226	27.034581277031517	30.712978396574826	22.16346101469643
43	20.642070028652046	26.89527023346516	29.64165876828883	22.821000969593968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	126.5
2	178.0
3	407.0
4	636.0
5	636.0
6	1135.0
7	1634.0
8	1860.0
9	2086.0
10	3346.5
11	4607.0
12	4607.0
13	8878.0
14	13149.0
15	21370.5
16	29592.0
17	28225.5
18	26859.0
19	26859.0
20	29710.0
21	32561.0
22	27119.5
23	21678.0
24	24484.5
25	27291.0
26	27291.0
27	30459.0
28	33627.0
29	36581.0
30	39535.0
31	43955.5
32	48376.0
33	48376.0
34	54064.0
35	59752.0
36	65114.0
37	70476.0
38	74258.0
39	78040.0
40	78040.0
41	80740.5
42	83441.0
43	87155.0
44	90869.0
45	96614.0
46	102359.0
47	102359.0
48	130721.0
49	159083.0
50	146170.5
51	133258.0
52	121830.0
53	110402.0
54	110402.0
55	96709.5
56	83017.0
57	70293.5
58	57570.0
59	51559.0
60	45548.0
61	45548.0
62	40362.0
63	35176.0
64	30443.0
65	25710.0
66	21754.5
67	17799.0
68	17799.0
69	15130.5
70	12462.0
71	10562.0
72	8662.0
73	7128.5
74	5595.0
75	5595.0
76	4453.0
77	3311.0
78	2626.5
79	1942.0
80	1516.5
81	1091.0
82	1091.0
83	837.5
84	584.0
85	430.5
86	277.0
87	217.5
88	158.0
89	158.0
90	136.5
91	115.0
92	82.5
93	50.0
94	31.5
95	13.0
96	13.0
97	10.0
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1468656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.07105702699367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.88606802415423	41.07680946721037
2	12.170274310321274	12.674380952937902
3	3.863487834276077	6.035276860250577
4	1.6784707947320885	3.495989938825519
5	0.877215010518056	2.283875641881027
6	0.5040669232579844	1.5748378503832645
7	0.34117556335017085	1.2435760550796406
8	0.2337765809265033	0.9738394941599643
9	0.1777685275599947	0.8330935622563062
>10	1.09390017041652	10.793129618641652
>50	0.1006962013940206	3.6346127605145244
>100	0.06301283262951313	6.031696438559552
>500	0.005895132348848011	2.1378460704442315
>1k	0.0036680823503943186	4.049560688319139
>5k	1.3100294108551138E-4	0.368563571110727
>10k+	3.9300882325653415E-4	2.792911029425596
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14684	0.9998256909718818	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14679	0.9994852436513383	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11580	0.7884759943785338	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5403	0.3678873745792071	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4546	0.3095347038380669	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4442	0.302453399570764	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4410	0.30027453671928617	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3993	0.27188123018596594	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3978	0.2708598882243357	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3714	0.2528842696996437	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2233	0.1520437733546862	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2164	0.14734560033118715	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2143	0.14591572158490485	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1932	0.131548844657973	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1861	0.1267144927062566	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1750	0.11915656219019294	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1739	0.11840757808499744	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1676	0.1141179418461505	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1662	0.11316468934862896	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1660	0.11302851042041158	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1632	0.11112200542536851	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1543	0.10506204311969583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.808946410868168E-5	0.0	0.0	6.808946410868168E-5	0.0
3	6.808946410868168E-5	0.0	0.0	6.808946410868168E-5	0.0
4	6.808946410868168E-5	0.0	0.0	6.808946410868168E-5	0.0
5	6.808946410868168E-5	0.0	0.0	1.3617892821736336E-4	0.0
6	6.808946410868168E-5	0.0	0.0	1.3617892821736336E-4	0.0
7	6.808946410868168E-5	0.0	0.0	1.3617892821736336E-4	0.0
8	1.3617892821736336E-4	0.0	0.0	1.3617892821736336E-4	0.0
9	1.3617892821736336E-4	6.808946410868168E-5	0.0	3.404473205434084E-4	0.0
10	1.3617892821736336E-4	6.808946410868168E-5	0.0	4.085367846520901E-4	0.0
11	1.3617892821736336E-4	6.808946410868168E-5	0.0	8.851630334128618E-4	0.0
12	1.3617892821736336E-4	6.808946410868168E-5	0.0	0.0012936998180649519	0.0
13	1.3617892821736336E-4	6.808946410868168E-5	0.0	0.0012936998180649519	0.0
14	2.723578564347267E-4	6.808946410868168E-5	0.0	0.0014298787462823153	0.0
15	2.723578564347267E-4	6.808946410868168E-5	0.0	0.001906504995043087	0.0
16	2.723578564347267E-4	6.808946410868168E-5	0.0	0.0026554891002385857	0.0
17	2.723578564347267E-4	6.808946410868168E-5	0.0	0.0036768310618688106	0.0
18	2.723578564347267E-4	6.808946410868168E-5	0.0	0.004153457310629582	0.0
19	3.404473205434084E-4	6.808946410868168E-5	0.0	0.0050386203440424444	0.0
20	3.404473205434084E-4	6.808946410868168E-5	0.0	0.007966467300715756	0.0
21	3.404473205434084E-4	6.808946410868168E-5	0.0	0.014094519070497108	0.0
22	3.404473205434084E-4	6.808946410868168E-5	0.0	0.022265254763538907	0.0
23	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.02791668028455949	0.0
24	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.03445326883899293	0.0
25	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.03915144186249197	0.0
26	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.047322177555533765	0.0
27	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.1025427329476746	0.0
28	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.20549400268000131	0.0
29	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.32880402218082383	0.0
30	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.5312339989759345	0.0
31	3.404473205434084E-4	1.3617892821736336E-4	0.0	0.7810542427906876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3700	0.0	29.8	1
ATAACGC	40	0.0019314411	23.125002	3
GTATTGG	545	0.0	20.027523	1
TTAACGG	150	0.0	19.733334	35
GTATTAG	295	0.0	18.186441	1
GTATCAA	6135	0.0	17.881824	2
ATTGGAC	615	0.0	16.845528	3
CCGAAAT	100	5.8800306E-6	16.65	24
TGGACCC	875	0.0	16.491428	5
GTCGGTT	180	1.0913936E-11	16.444445	12
GGACCCT	865	0.0	16.254335	6
TATGCCG	115	1.2426935E-6	16.086956	10
CGACGAA	150	4.6729838E-9	16.033333	10
TTGGACC	860	0.0	15.918604	4
GTAAGAC	70	0.0025929532	15.857143	3
CGAACTA	200	3.6379788E-12	15.724999	24
TCCGACG	95	7.0618116E-5	15.578948	23
ACGACAT	95	7.0618116E-5	15.578948	27
GACCCTC	930	0.0	15.118279	7
TAAGCCG	135	3.974037E-7	15.074073	10
>>END_MODULE