FastQCFastQC Report
Wed 25 May 2016
SRR2088100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787784
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT291721.6317407472043601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT275161.53911210750292No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT214511.1998653081132844No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102380.5726642592169972No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG70460.39411920008233653No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA65530.3665431618137314No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58850.32917846898730496No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG56380.31536248226855146No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA49040.2743060682946038No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42960.24029748560228753No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38280.21411982655622827No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.1964443131832481No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32250.18039091970842117No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29630.16573590545614011No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA29030.16237979532202995No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26750.14962657681241134No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA24930.1394463760722772No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT22690.1269168982382659No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.12467949148219248No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22260.12451168597548697No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20940.11712824368044462No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG20910.1169604381737391No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG19710.1102482179055188No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19670.11002447722991145No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC18760.10493437685984437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70100.028.502141
GTATTGG6150.021.3577231
CCCGTAT656.904872E-519.9230771
TACGTTA951.6774175E-719.47368419
CGCCGGA1457.2759576E-1219.1379314
TATACCG1351.1532393E-917.8148145
CGTATAC852.724485E-517.4117643
GTATCAA115200.017.3116322
CGACCGC1401.87174E-917.17857210
TAAGCCG1107.81356E-716.81818210
AGCCGTC1352.2215318E-816.44444312
ATTGGAC7750.016.2322583
ACCGCCG1606.2937033E-1016.187512
GGACCCT9100.016.060446
TGGACCC10100.015.7524755
GACCCTC9050.015.7403327
TTGGACC10200.015.5980394
TCAAGAC3050.015.1639343
CCGACCG1601.0975782E-815.031259
TCGGCCG1252.9607854E-614.80000127
Produced by FastQC (version 0.11.5)