##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088100_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1787784
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503130132051748	31.0	31.0	34.0	30.0	34.0
2	31.697048972359077	31.0	31.0	34.0	30.0	34.0
3	31.744832709096848	31.0	31.0	34.0	30.0	34.0
4	35.45344292151625	37.0	35.0	37.0	33.0	37.0
5	35.3568468002846	37.0	35.0	37.0	33.0	37.0
6	35.409286580481755	37.0	35.0	37.0	33.0	37.0
7	35.25745447995955	37.0	35.0	37.0	32.0	37.0
8	35.320921878705704	37.0	35.0	37.0	32.0	37.0
9	36.83265875519638	39.0	37.0	39.0	32.0	39.0
10	36.676086708461426	39.0	35.0	39.0	32.0	39.0
11	36.73101448497134	39.0	35.0	39.0	32.0	39.0
12	36.6029604247493	39.0	35.0	39.0	32.0	39.0
13	36.63879529070626	39.0	35.0	39.0	32.0	39.0
14	37.55574946414108	39.0	36.0	41.0	32.0	41.0
15	37.56599846513897	39.0	36.0	41.0	32.0	41.0
16	37.43566952159769	39.0	36.0	41.0	32.0	41.0
17	37.5076351505551	39.0	36.0	41.0	32.0	41.0
18	37.50516673155146	39.0	36.0	41.0	32.0	41.0
19	37.570609201111544	39.0	37.0	41.0	32.0	41.0
20	37.50042063247014	39.0	36.0	41.0	32.0	41.0
21	37.42792361940816	39.0	36.0	41.0	32.0	41.0
22	37.31200133796924	39.0	36.0	41.0	31.0	41.0
23	37.097145404590265	39.0	36.0	40.0	31.0	41.0
24	36.938712394785945	39.0	36.0	40.0	31.0	41.0
25	36.775319613555105	39.0	35.0	40.0	30.0	41.0
26	36.91203747208835	39.0	36.0	40.0	31.0	41.0
27	36.82351055832248	39.0	35.0	40.0	30.0	41.0
28	36.72958422270252	39.0	35.0	40.0	30.0	41.0
29	36.621848053232384	39.0	35.0	40.0	30.0	41.0
30	36.351737681957104	38.0	35.0	40.0	30.0	41.0
31	36.10479342023421	38.0	35.0	40.0	30.0	41.0
32	35.92578857401118	38.0	35.0	40.0	29.0	41.0
33	35.72114975858381	38.0	35.0	40.0	29.0	41.0
34	35.57214685890465	38.0	34.0	40.0	27.0	41.0
35	35.34913054373459	38.0	34.0	40.0	27.0	41.0
36	35.23320266877878	38.0	34.0	40.0	26.0	41.0
37	35.02782494977022	38.0	34.0	40.0	25.0	41.0
38	34.87916996684163	38.0	33.0	40.0	24.0	41.0
39	34.732822309630244	38.0	33.0	40.0	24.0	41.0
40	34.50551464830203	38.0	33.0	40.0	23.0	41.0
41	34.294823647599486	38.0	33.0	40.0	21.0	41.0
42	34.02986714278683	38.0	33.0	40.0	20.0	41.0
43	33.665585999203486	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	3.0
14	4.0
15	10.0
16	27.0
17	60.0
18	163.0
19	349.0
20	715.0
21	1541.0
22	2877.0
23	4841.0
24	7743.0
25	12383.0
26	17403.0
27	24312.0
28	33116.0
29	42184.0
30	53039.0
31	65162.0
32	79319.0
33	98175.0
34	119272.0
35	144398.0
36	186112.0
37	253676.0
38	320880.0
39	320018.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.789370527983245	22.658553829769144	15.685172257946151	21.86690338430146
2	18.778498968555486	24.575787679048474	35.28944212499944	21.356271227396597
3	19.353904051048673	24.997650722906123	34.36377101484296	21.284674211202248
4	13.552979554577064	17.271269907326612	36.312160753200615	32.863589784895716
5	11.600842159902985	39.59879940753469	35.01854810200785	13.781810330554475
6	30.952844415208997	38.36956813574795	13.1275925950786	17.549994853964463
7	24.047480008770634	35.046403816120964	21.43670600027744	19.469410174830966
8	26.680236538642255	35.38811176294228	18.787952012099897	19.143699686315575
9	23.69575966671589	14.996274717751138	22.98566269750708	38.32230291802589
10	16.347500592912787	28.891969052189747	33.06836843824534	21.692161916652122
11	31.900442111575	23.2755746779253	23.94472710349796	20.87925610700174
12	20.727392123433255	28.647588299257627	31.07881041557593	19.546209161733184
13	31.61192851037933	22.0020986875372	27.125872029283183	19.260100772800293
14	19.610478670801395	24.388908279747444	29.126617085732953	26.87399596371821
15	21.674877949461457	30.52281483669168	26.961087021698372	20.841220192148494
16	20.411414354306785	29.459151664854367	27.985204029122084	22.144229951716763
17	20.671624760038128	27.79401762181561	27.917242798906354	23.61711481923991
18	22.147697932188677	25.878070281421024	31.28414842061457	20.69008336577573
19	21.82126028647756	25.02287748408085	32.94670944588384	20.209152783557744
20	22.7729971853423	24.37212772907689	32.91124654880008	19.94362853678073
21	21.366115817123323	25.590731318772292	33.65798105363959	19.3851718104648
22	19.691025314020038	25.802389997896835	33.13935016758177	21.36723452050136
23	21.12111977733328	26.0661802544379	34.04930349527683	18.76339647295199
24	20.793171882061813	26.090791728754702	31.53848563361122	21.57755075557226
25	20.55169975791259	26.743331409163524	32.61971244848371	20.08525638444018
26	20.3752243000273	27.570444751714973	32.33147852313255	19.722852425125183
27	19.074675687890707	27.46444760664599	32.063325323417146	21.397551382046153
28	19.65058418690401	27.016015357559976	32.21983192600448	21.11356852953153
29	19.93792314955274	26.59879493272118	31.43808200543242	22.025199912293655
30	20.36163205398415	26.890049357193043	33.34686964420758	19.401448944615233
31	21.35973920786851	26.77644502915341	32.74081208915619	19.12300367382189
32	20.66508034527661	26.64427022503837	31.91112572883525	20.779523700849765
33	20.021266551216478	26.546048068446748	32.72643675074841	20.706248629588362
34	19.02595615577721	27.152497169680455	33.60596134656088	20.215585327981454
35	18.608847601276217	29.317020400674803	32.052585770987996	20.021546227060988
36	21.30716014910079	26.868178706152424	31.718876553319642	20.105784591427152
37	20.362582951855483	27.72818192801815	31.722064857947046	20.187170262179325
38	20.003199491661185	26.03071735735413	33.136833084981184	20.8292500660035
39	19.47142384091143	25.75976739919364	34.001590796203566	20.767217963691365
40	18.242360374631385	26.302226667203644	33.47361873693914	21.98179422122583
41	19.93697225168141	25.429917708179513	33.45062938252049	21.182480657618594
42	18.628201169716252	27.68723738438201	32.52920934520054	21.1553521007012
43	19.09609885758011	27.746304922742343	31.42577626827402	21.731819951403526
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	262.0
2	350.0
3	639.0
4	928.0
5	928.0
6	1814.0
7	2700.0
8	3172.5
9	3645.0
10	6047.0
11	8449.0
12	8449.0
13	16707.5
14	24966.0
15	41308.5
16	57651.0
17	53179.0
18	48707.0
19	48707.0
20	53397.5
21	58088.0
22	46308.5
23	34529.0
24	38371.0
25	42213.0
26	42213.0
27	45183.0
28	48153.0
29	50343.5
30	52534.0
31	56783.0
32	61032.0
33	61032.0
34	66872.0
35	72712.0
36	78492.0
37	84272.0
38	87951.0
39	91630.0
40	91630.0
41	93519.0
42	95408.0
43	98998.0
44	102588.0
45	110741.5
46	118895.0
47	118895.0
48	160877.0
49	202859.0
50	181644.5
51	160430.0
52	140820.0
53	121210.0
54	121210.0
55	104633.5
56	88057.0
57	73379.5
58	58702.0
59	51706.0
60	44710.0
61	44710.0
62	38968.0
63	33226.0
64	28416.0
65	23606.0
66	19893.5
67	16181.0
68	16181.0
69	13718.5
70	11256.0
71	9123.5
72	6991.0
73	5772.5
74	4554.0
75	4554.0
76	3648.0
77	2742.0
78	2131.0
79	1520.0
80	1234.5
81	949.0
82	949.0
83	730.5
84	512.0
85	400.0
86	288.0
87	230.0
88	172.0
89	172.0
90	142.5
91	113.0
92	79.5
93	46.0
94	34.5
95	23.0
96	23.0
97	15.0
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1787784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.7979033511473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52711709101423	35.047815122762024
2	12.945782339518392	11.857793767805056
3	4.499478830775978	6.182000898672344
4	2.002309039920239	3.6680622355758254
5	1.0647818482637876	2.438238808842047
6	0.6267899923462014	1.7223400494562613
7	0.39934703845654984	1.2802479949559982
8	0.270133522772791	0.9897239174282585
9	0.20013094208188362	0.8249019788736147
>10	1.2280109885634662	10.8576810439329
>50	0.13927930687364282	4.465227347310955
>100	0.08435183304405679	7.450514062047953
>500	0.005753918032663042	1.8997921212776239
>1k	0.005753918032663042	4.952052282127363
>5k	4.896951517160035E-4	1.4085284402885276
>10k+	4.896951517160035E-4	4.955079928643388
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	29172	1.6317407472043601	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27516	1.53911210750292	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21451	1.1998653081132844	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10238	0.5726642592169972	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7046	0.39411920008233653	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6553	0.3665431618137314	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5885	0.32917846898730496	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5638	0.31536248226855146	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4904	0.2743060682946038	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4296	0.24029748560228753	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3828	0.21411982655622827	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3512	0.1964443131832481	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3225	0.18039091970842117	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2963	0.16573590545614011	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2903	0.16237979532202995	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2675	0.14962657681241134	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2493	0.1394463760722772	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2269	0.1269168982382659	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2229	0.12467949148219248	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2226	0.12451168597548697	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2094	0.11712824368044462	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2091	0.1169604381737391	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1971	0.1102482179055188	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1967	0.11002447722991145	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1876	0.10493437685984437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	5.593516890183602E-5	0.0	0.0	0.0	0.0
6	5.593516890183602E-5	0.0	0.0	0.0	0.0
7	5.593516890183602E-5	0.0	0.0	0.0	0.0
8	1.6780550670550805E-4	0.0	0.0	0.0	0.0
9	1.6780550670550805E-4	0.0	5.593516890183602E-5	1.1187033780367204E-4	0.0
10	1.6780550670550805E-4	0.0	5.593516890183602E-5	3.356110134110161E-4	0.0
11	1.6780550670550805E-4	0.0	5.593516890183602E-5	6.712220268220322E-4	0.0
12	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.0011187033780367204	0.0
13	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.0012305737158403924	0.0
14	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.0013424440536440644	0.0
15	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.0019017957426624246	0.0
16	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.002517082600582621	0.0
17	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.003579850809717505	0.0
18	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.004139202498735865	0.0
19	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.0053697762145762575	0.0
20	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.008725886348686419	0.0
21	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.013927857056557169	0.0
22	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.020751947662581163	0.0
23	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.025842048032648238	0.0
24	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.03244239796306489	0.0
25	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.03842746103556134	0.0
26	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.047377088059855105	0.0
27	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.11036008824332245	0.0
28	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.24264676269616464	0.0
29	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.405921520720624	0.0
30	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.6727322763823818	0.0
31	1.6780550670550805E-4	0.0	5.593516890183602E-5	0.9817740845650258	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7010	0.0	28.50214	1
GTATTGG	615	0.0	21.357723	1
CCCGTAT	65	6.904872E-5	19.923077	1
TACGTTA	95	1.6774175E-7	19.473684	19
CGCCGGA	145	7.2759576E-12	19.13793	14
TATACCG	135	1.1532393E-9	17.814814	5
CGTATAC	85	2.724485E-5	17.411764	3
GTATCAA	11520	0.0	17.311632	2
CGACCGC	140	1.87174E-9	17.178572	10
TAAGCCG	110	7.81356E-7	16.818182	10
AGCCGTC	135	2.2215318E-8	16.444443	12
ATTGGAC	775	0.0	16.232258	3
ACCGCCG	160	6.2937033E-10	16.1875	12
GGACCCT	910	0.0	16.06044	6
TGGACCC	1010	0.0	15.752475	5
GACCCTC	905	0.0	15.740332	7
TTGGACC	1020	0.0	15.598039	4
TCAAGAC	305	0.0	15.163934	3
CCGACCG	160	1.0975782E-8	15.03125	9
TCGGCCG	125	2.9607854E-6	14.800001	27
>>END_MODULE