FastQCFastQC Report
Wed 25 May 2016
SRR2088099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735461
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT395861.4471418163154217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT384391.4052110412102385No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT300131.0971825224340612No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137350.5021091508890092No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG103350.3778156588596949No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG95880.3505076475226662No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA94230.34447575746830245No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG84650.3094542382435721No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA80210.2932229704609205No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA73500.26869328423984107No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA58410.21352890792447782No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC46790.17104977917798864No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45120.1649447753047841No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43830.16022893398955423No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA43560.15924189743520378No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA42920.15690225523229906No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39010.1426085036489279No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA35430.12952113007642954No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG35220.1287534349786014No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG32180.11764013451480389No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31960.1168358825075554No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG31340.11456935412349142No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31010.11336297611261867No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC30010.10970728517058002No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT29670.10846435025028689No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT28220.10316359838433084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92250.029.9409221
GTATTGG7250.020.1586191
ATTCGCG851.2465935E-619.58823629
GTATCAA155050.017.7900682
ACCGTCG1258.592906E-917.7623
ATTGGAC9100.017.4835173
TTGGACC14950.015.715724
TTAACGG2750.015.47272835
GGACCCT14800.015.256
TAACGGC2700.015.07407536
TATACTG4100.014.8902445
TATACCG1904.5656634E-1014.6052645
TTCGCGT1152.2119384E-514.47826130
TCGCGTC1152.2119384E-514.47826131
TGGACCC15350.014.3420195
GATACTG11900.014.30252136
GACCGTG1854.9076334E-914.07
GACCGTC1601.7860475E-713.87522
GATACCT10850.013.8110636
CGAACTA2700.013.70370424
Produced by FastQC (version 0.11.5)