##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088099_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2735461
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50895552888526	31.0	31.0	34.0	30.0	34.0
2	31.699158935184965	31.0	31.0	34.0	30.0	34.0
3	31.751411553664994	31.0	31.0	34.0	30.0	34.0
4	35.46961919764164	37.0	35.0	37.0	33.0	37.0
5	35.364785679634984	37.0	35.0	37.0	33.0	37.0
6	35.409748484807494	37.0	35.0	37.0	33.0	37.0
7	35.26643516394494	37.0	35.0	37.0	32.0	37.0
8	35.296485309057594	37.0	35.0	37.0	32.0	37.0
9	36.8307294456035	39.0	37.0	39.0	32.0	39.0
10	36.67204906229699	39.0	35.0	39.0	32.0	39.0
11	36.744024133409326	39.0	35.0	39.0	32.0	39.0
12	36.61595138808413	39.0	35.0	39.0	32.0	39.0
13	36.65872114426051	39.0	35.0	39.0	32.0	39.0
14	37.585852256712855	39.0	36.0	41.0	32.0	41.0
15	37.60087129737913	39.0	36.0	41.0	32.0	41.0
16	37.4632959490192	39.0	36.0	41.0	32.0	41.0
17	37.52242345988483	39.0	36.0	41.0	32.0	41.0
18	37.51619928048691	39.0	36.0	41.0	32.0	41.0
19	37.56648988963835	39.0	36.0	41.0	32.0	41.0
20	37.493002459183295	39.0	36.0	41.0	32.0	41.0
21	37.41498453094378	39.0	36.0	41.0	32.0	41.0
22	37.33031690088069	39.0	36.0	41.0	31.0	41.0
23	37.126429512246744	39.0	36.0	40.0	31.0	41.0
24	36.9850204407959	39.0	36.0	40.0	31.0	41.0
25	36.833195574713	39.0	35.0	40.0	30.0	41.0
26	36.92723164395325	39.0	36.0	40.0	31.0	41.0
27	36.841885883220414	39.0	35.0	40.0	31.0	41.0
28	36.73824887285909	39.0	35.0	40.0	30.0	41.0
29	36.62502371629498	39.0	35.0	40.0	30.0	41.0
30	36.381048020790644	38.0	35.0	40.0	30.0	41.0
31	36.17240640608658	38.0	35.0	40.0	30.0	41.0
32	36.00598326936483	38.0	35.0	40.0	30.0	41.0
33	35.83118055786575	38.0	35.0	40.0	29.0	41.0
34	35.69019115973505	38.0	34.0	40.0	28.0	41.0
35	35.469695967151424	38.0	34.0	40.0	27.0	41.0
36	35.3386039866772	38.0	34.0	40.0	26.0	41.0
37	35.165068337658624	38.0	34.0	40.0	25.0	41.0
38	35.02111051848299	38.0	34.0	40.0	25.0	41.0
39	34.91185507671285	38.0	33.0	40.0	24.0	41.0
40	34.68796996191867	38.0	33.0	40.0	23.0	41.0
41	34.51596348842115	38.0	33.0	40.0	23.0	41.0
42	34.27339413722221	38.0	33.0	40.0	21.0	41.0
43	33.909358970937625	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	2.0
13	1.0
14	4.0
15	10.0
16	39.0
17	99.0
18	208.0
19	501.0
20	1037.0
21	2115.0
22	3903.0
23	6780.0
24	11232.0
25	17280.0
26	25533.0
27	35727.0
28	48831.0
29	63549.0
30	80257.0
31	99376.0
32	121856.0
33	149574.0
34	183350.0
35	221719.0
36	284573.0
37	380484.0
38	483602.0
39	513817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16642898582725	22.090170541638138	15.112260785293593	22.631139687241017
2	18.774020174296034	23.912313134787887	35.356014945926844	21.957651744989235
3	19.387189216004177	24.580792780449073	33.851771237096784	22.180246766449972
4	13.535488168173481	16.74825559567473	36.126671153418016	33.58958508273377
5	11.887246793136514	39.10021016567226	35.17520447193361	13.837338569257613
6	31.47856979134413	37.67299186499095	13.253780624179983	17.594657719484943
7	24.754620884743012	34.30730688538422	21.4264432942016	19.511628935671173
8	26.708295237987308	34.9298710528134	18.958413225412464	19.403420483786828
9	23.884054643805925	14.771440718767332	22.734741968538394	38.60976266888835
10	16.27159005374231	28.320601171064038	33.45841889173342	21.94938988346023
11	32.31480909433547	22.732109871060125	23.93282156097272	21.020259473631683
12	20.965460666410525	28.03414122884589	30.97569294535729	20.024705159386297
13	31.658027659688802	21.52598775855331	27.019760106249002	19.79622447550888
14	19.94811843415059	23.69611557247572	28.718523130104945	27.637242863268753
15	21.82275674922801	30.065133445514302	26.70760065670832	21.404509148549366
16	21.005673266772952	28.902733396674275	27.512766586692333	22.578826749860443
17	21.02632792059547	27.220969335698808	27.595019632888206	24.15768311081752
18	22.642362658433075	25.552694774299468	30.726155481653734	21.078787085613722
19	22.161127502823106	24.70625609357984	32.26183813258533	20.870778271011723
20	23.174923714869266	24.071226020038306	32.3327950937703	20.421055171322127
21	21.870426959112194	25.127611031559216	32.96464471619226	20.03731729313633
22	20.093614933643725	25.451066566110796	32.50201702747727	21.95330147276821
23	21.627506296013724	25.611003044824987	33.42350704323695	19.337983615924337
24	21.33764656121948	25.678487099615023	30.858820505940315	22.125045833225183
25	21.18626439930966	26.221064749232397	32.013945729805684	20.578725121652255
26	20.85937251527256	27.050102341067923	31.759399969511538	20.331125174147978
27	19.508704382917543	27.01606785839754	31.446911507786073	22.028316250898843
28	20.237027689299904	26.392077971500967	31.61145415708723	21.7594401821119
29	20.582417369503712	26.08002819268854	30.809176222947432	22.52837821486031
30	20.829432406457265	26.49637483407733	32.61779275961164	20.05639999985377
31	21.95523898896749	26.211157826779473	32.15611555054157	19.677487633711465
32	21.13157526281676	26.194195420808413	31.134605830607708	21.539623485767116
33	20.646136062623448	26.096990598659602	31.95918347949395	21.297689859223
34	19.611977652030134	26.741123342646816	32.89525970211237	20.751639303210684
35	19.291446670232183	28.914431607688794	31.327333857071988	20.46678786500703
36	21.88150370266657	26.309166900935526	31.083901397241636	20.725427999156267
37	20.911941351019077	27.242135786253215	30.990352266034865	20.855570596692843
38	20.62851563228282	25.598573695622058	32.425613086788665	21.347297585306464
39	20.104618563379262	25.309371985197377	33.25315184533795	21.33285760608541
40	18.768390410245292	26.01517623537678	32.68268127383282	22.533752080545106
41	20.584208658065315	25.019950933316178	32.782554750369314	21.613285658249197
42	19.157136585021682	27.401158342231895	31.81196880525805	21.62973626748837
43	19.664948613780272	27.344422018811454	30.79575983719015	22.194869530218124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	219.0
1	371.5
2	524.0
3	976.0
4	1428.0
5	1428.0
6	2664.0
7	3900.0
8	4473.5
9	5047.0
10	8232.0
11	11417.0
12	11417.0
13	22540.0
14	33663.0
15	55915.0
16	78167.0
17	72768.5
18	67370.0
19	67370.0
20	73446.0
21	79522.0
22	63279.5
23	47037.0
24	51685.0
25	56333.0
26	56333.0
27	61335.0
28	66337.0
29	69551.5
30	72766.0
31	79500.5
32	86235.0
33	86235.0
34	95344.0
35	104453.0
36	114744.0
37	125035.0
38	132116.5
39	139198.0
40	139198.0
41	143154.5
42	147111.0
43	154314.5
44	161518.0
45	174862.5
46	188207.0
47	188207.0
48	253124.5
49	318042.0
50	286496.5
51	254951.0
52	225342.5
53	195734.0
54	195734.0
55	169384.0
56	143034.0
57	119177.0
58	95320.0
59	85251.5
60	75183.0
61	75183.0
62	66260.0
63	57337.0
64	49260.5
65	41184.0
66	34524.5
67	27865.0
68	27865.0
69	23625.5
70	19386.0
71	16119.5
72	12853.0
73	10414.0
74	7975.0
75	7975.0
76	6460.0
77	4945.0
78	3804.0
79	2663.0
80	2157.5
81	1652.0
82	1652.0
83	1254.0
84	856.0
85	673.5
86	491.0
87	374.0
88	257.0
89	257.0
90	214.0
91	171.0
92	107.0
93	43.0
94	30.0
95	17.0
96	17.0
97	12.0
98	7.0
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2735461.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.09771626886283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67997854276815	31.017588313893558
2	13.52962960310195	11.391330165083865
3	5.241481742302624	6.61963233647642
4	2.51574488436153	4.236284573867794
5	1.3418939210501584	2.82453347756407
6	0.8348859125585367	2.108807415825594
7	0.5233767338942967	1.542307567164436
8	0.37439194087176425	1.2608836560134722
9	0.255466853898686	0.9679114018373328
>10	1.4419141080590032	11.489618767423119
>50	0.14399454591310124	4.233993467893347
>100	0.10256432780426804	8.161951803412549
>500	0.007381864439942931	2.081687393149758
>1k	0.006339718871950338	5.453575669775347
>5k	5.210727839959181E-4	1.7800311877213753
>10k+	4.342273199965985E-4	4.829862802897951
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39586	1.4471418163154217	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38439	1.4052110412102385	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30013	1.0971825224340612	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13735	0.5021091508890092	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10335	0.3778156588596949	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9588	0.3505076475226662	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9423	0.34447575746830245	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8465	0.3094542382435721	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8021	0.2932229704609205	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7350	0.26869328423984107	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5841	0.21352890792447782	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4679	0.17104977917798864	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4512	0.1649447753047841	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4383	0.16022893398955423	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4356	0.15924189743520378	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4292	0.15690225523229906	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3901	0.1426085036489279	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3543	0.12952113007642954	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3522	0.1287534349786014	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3218	0.11764013451480389	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3196	0.1168358825075554	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3134	0.11456935412349142	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3101	0.11336297611261867	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3001	0.10970728517058002	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2967	0.10846435025028689	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2822	0.10316359838433084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.6556909420386545E-5	0.0	0.0	0.0	0.0
7	3.6556909420386545E-5	0.0	0.0	3.6556909420386545E-5	0.0
8	3.6556909420386545E-5	0.0	0.0	3.6556909420386545E-5	0.0
9	7.311381884077309E-5	0.0	0.0	1.4622763768154618E-4	0.0
10	7.311381884077309E-5	0.0	0.0	2.5589836594270583E-4	0.0
11	7.311381884077309E-5	0.0	0.0	5.483536413057982E-4	0.0
12	7.311381884077309E-5	0.0	0.0	9.139227355096637E-4	0.0
13	7.311381884077309E-5	0.0	0.0	0.0010601503731912099	0.0
14	7.311381884077309E-5	0.0	0.0	0.0012429349202931425	0.0
15	7.311381884077309E-5	0.0	0.0	0.0017181747427581677	0.0
16	7.311381884077309E-5	0.0	0.0	0.0024493129311658987	0.0
17	7.311381884077309E-5	0.0	0.0	0.0033266787572551757	0.0
18	7.311381884077309E-5	0.0	0.0	0.0038750323985609738	0.0
19	7.311381884077309E-5	0.0	0.0	0.005008296590592957	0.0
20	7.311381884077309E-5	0.0	0.0	0.008334975347848132	0.0
21	7.311381884077309E-5	0.0	0.0	0.012868032115976064	0.0
22	7.311381884077309E-5	0.0	0.0	0.019521389630486417	0.0
23	7.311381884077309E-5	0.0	0.0	0.023981332579773574	0.0
24	7.311381884077309E-5	0.0	0.0	0.029684210449353876	0.0
25	7.311381884077309E-5	0.0	0.0	0.03414415339864103	0.0
26	7.311381884077309E-5	0.0	0.0	0.04313715311605612	0.0
27	7.311381884077309E-5	0.0	0.0	0.10389473657273857	0.0
28	7.311381884077309E-5	0.0	0.0	0.22584858639914807	0.0
29	1.0967072826115964E-4	0.0	0.0	0.37353850045750975	0.0
30	1.4622763768154618E-4	0.0	0.0	0.6228931796139664	0.0
31	1.4622763768154618E-4	0.0	0.0	0.9082564145495037	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9225	0.0	29.940922	1
GTATTGG	725	0.0	20.158619	1
ATTCGCG	85	1.2465935E-6	19.588236	29
GTATCAA	15505	0.0	17.790068	2
ACCGTCG	125	8.592906E-9	17.76	23
ATTGGAC	910	0.0	17.483517	3
TTGGACC	1495	0.0	15.71572	4
TTAACGG	275	0.0	15.472728	35
GGACCCT	1480	0.0	15.25	6
TAACGGC	270	0.0	15.074075	36
TATACTG	410	0.0	14.890244	5
TATACCG	190	4.5656634E-10	14.605264	5
TTCGCGT	115	2.2119384E-5	14.478261	30
TCGCGTC	115	2.2119384E-5	14.478261	31
TGGACCC	1535	0.0	14.342019	5
GATACTG	1190	0.0	14.302521	36
GACCGTG	185	4.9076334E-9	14.0	7
GACCGTC	160	1.7860475E-7	13.875	22
GATACCT	1085	0.0	13.81106	36
CGAACTA	270	0.0	13.703704	24
>>END_MODULE