FastQCFastQC Report
Wed 25 May 2016
SRR2088098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286772
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.7980434762335519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100270.7792367256981035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79310.6163485061844678No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.297488599378911No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG24730.1921863391494375No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA22870.1777315639445061No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG22670.17617728704075003No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG20500.15931338263499673No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA18060.14035120440917273No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16670.12954897992806808No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA13520.10506911869391003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.029.0502011
ACGTTTA809.786163E-1025.43750226
CGTTTAT1052.2600943E-819.3809527
TGGCGTA609.237634E-418.55
GCGACTA500.007035329518.49999835
TAAGCCG1002.8755858E-718.49999836
CTGCGTA852.723526E-517.4117649
TAAAACG650.00158007317.0769234
CGAGCTA650.00158007317.07692322
ACGACGC650.00158007317.07692328
GTATCAA43300.016.7055442
TATACGG904.4466207E-516.4444452
TTGGCGT700.002592719215.8571434
TCACGTT1302.5894587E-715.65384624
GTCTCAC1353.9729093E-715.0740751
ACACCGC750.004104760514.86
AATCGGC750.004104760514.825
AGTACCG1001.0935253E-414.7999995
ATTGGAC2800.014.5357153
TCAAGAC2750.014.1272743
Produced by FastQC (version 0.11.5)