##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1286772
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.646834870513192	31.0	31.0	34.0	30.0	34.0
2	31.85487405694249	31.0	31.0	34.0	30.0	34.0
3	31.932863009142256	31.0	31.0	34.0	30.0	34.0
4	35.635336329979204	37.0	35.0	37.0	33.0	37.0
5	35.505451626239925	37.0	35.0	37.0	33.0	37.0
6	35.533870025148204	37.0	35.0	37.0	33.0	37.0
7	35.41434535411091	37.0	35.0	37.0	33.0	37.0
8	35.41326357738589	37.0	35.0	37.0	33.0	37.0
9	37.010401221039935	39.0	37.0	39.0	33.0	39.0
10	36.83470964553161	39.0	37.0	39.0	32.0	39.0
11	36.89905904076247	39.0	37.0	39.0	33.0	39.0
12	36.77972321436898	39.0	37.0	39.0	32.0	39.0
13	36.807115790520776	39.0	37.0	39.0	32.0	39.0
14	37.8499741990034	40.0	37.0	41.0	33.0	41.0
15	37.8528309599525	40.0	37.0	41.0	33.0	41.0
16	37.74053523079458	39.0	37.0	41.0	32.0	41.0
17	37.713311293686836	39.0	37.0	41.0	32.0	41.0
18	37.696016854578744	39.0	37.0	41.0	32.0	41.0
19	37.71151221817074	39.0	37.0	41.0	32.0	41.0
20	37.6278027498267	39.0	37.0	41.0	32.0	41.0
21	37.53531783408405	39.0	36.0	41.0	32.0	41.0
22	37.43419735586413	39.0	36.0	41.0	32.0	41.0
23	37.235328403166996	39.0	36.0	41.0	31.0	41.0
24	37.10648661923013	39.0	36.0	41.0	31.0	41.0
25	36.97778161166081	39.0	36.0	40.0	31.0	41.0
26	37.116604961873584	39.0	36.0	41.0	31.0	41.0
27	37.049676244120946	39.0	36.0	41.0	31.0	41.0
28	36.976001964606006	39.0	36.0	41.0	31.0	41.0
29	36.87967487635727	39.0	36.0	41.0	30.0	41.0
30	36.70133947583566	39.0	35.0	40.0	30.0	41.0
31	36.56228686977957	39.0	35.0	40.0	30.0	41.0
32	36.4213963312848	38.0	35.0	40.0	30.0	41.0
33	36.284497953017315	38.0	35.0	40.0	30.0	41.0
34	36.1643018343576	38.0	35.0	40.0	30.0	41.0
35	35.99294513713385	38.0	35.0	40.0	29.0	41.0
36	35.888499283478346	38.0	35.0	40.0	28.0	41.0
37	35.750725070175605	38.0	35.0	40.0	27.0	41.0
38	35.680970677011935	38.0	35.0	40.0	27.0	41.0
39	35.6159910224966	38.0	35.0	40.0	27.0	41.0
40	35.477487853326	38.0	34.0	40.0	26.0	41.0
41	35.378377055142636	38.0	34.0	40.0	25.0	41.0
42	35.22795569067403	38.0	34.0	40.0	25.0	41.0
43	34.90105473230689	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	3.0
15	6.0
16	16.0
17	34.0
18	91.0
19	180.0
20	407.0
21	835.0
22	1544.0
23	2572.0
24	4277.0
25	6557.0
26	9732.0
27	13804.0
28	19115.0
29	25363.0
30	33009.0
31	41762.0
32	52072.0
33	64605.0
34	80941.0
35	100351.0
36	130404.0
37	175393.0
38	245722.0
39	277973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16702259607763	21.910641512249256	13.950567777352942	23.971768114320174
2	18.62971839611058	23.011768984715243	35.608794720432215	22.74971789874197
3	18.89884144199594	24.801052556319224	32.522389358798605	23.77771664288623
4	13.84837407093098	16.822716067803775	36.85338195111488	32.47552791015036
5	12.677226423950785	38.227595875570806	35.579263459260844	13.515914241217558
6	31.912413387919536	38.15539971339134	14.349317517011562	15.582869381677561
7	25.890212096626286	33.00032950670359	22.325555731706938	18.78390266496318
8	25.891766373530047	35.62682433251578	19.607513996263517	18.873895297690655
9	24.702355972930715	14.773868253272529	21.905279256931298	38.618496516865456
10	16.01868862549076	27.920564016002835	34.27437028471244	21.786377073793957
11	33.417730569207286	22.91983350585807	23.406322176733717	20.256113748200924
12	20.51699912649638	27.217953141659905	31.102712834907813	21.1623348969359
13	30.114115010273768	21.733298517530688	26.312819986757564	21.83976648543798
14	20.4104534447439	22.8348922730678	27.70490809560668	29.049746186581615
15	22.705265579294544	29.772873516054126	25.20019086520378	22.321670039447547
16	22.175101727423353	28.603124718287308	26.269455661142764	22.95231789314657
17	21.544687015259893	27.51528631334844	27.244686704404508	23.69533996698716
18	22.52147233542539	26.61854625372638	29.10329102591601	21.756690384932217
19	22.700214179357335	25.74853975684892	30.03764458660897	21.513601477184768
20	23.299465639600488	25.499000599950882	29.788416285091685	21.41311747535694
21	22.33682423925917	26.121255358369627	30.191828855461573	21.35009154690963
22	21.689545622689955	26.386259570460034	29.685600867908224	22.238593938941786
23	22.397751893886408	26.29510123005474	30.280344925130482	21.02680195092837
24	22.222818028368664	26.554820900672382	28.71138010463392	22.510980966325036
25	22.263384655556695	26.573394509672266	29.596463087477815	21.566757747293227
26	21.969082323830484	27.16876027765603	29.402877899114994	21.459279499398495
27	21.357785217583224	27.085528749459886	29.094975644480918	22.461710388475968
28	21.60343868222187	26.778636774813254	29.38927797620713	22.22864656675775
29	21.849791571467208	26.618468539881192	29.026587460715653	22.50515242793595
30	21.930924825843274	26.734029027675454	29.919053258852387	21.41599288762889
31	22.45137444706599	26.58621729412825	29.888200862312825	21.07420739649293
32	21.812333498086687	26.501897772099486	29.3053470234043	22.38042170640953
33	21.743789886631042	26.384316724330343	29.806679038710822	22.065214350327796
34	21.176245675224514	26.507260027417445	30.435850329351275	21.880643968006762
35	21.10311694690279	27.56416832197157	29.526831482189543	21.805883248936098
36	22.476709160597217	26.204486886565764	29.374823201002197	21.943980751834825
37	21.609344934456143	26.56818768204468	29.588225419887905	22.23424196361127
38	21.543832162962826	25.67175847780337	30.519081857547413	22.26532750168639
39	21.16622058919529	25.540888362507108	31.06416676769467	22.228724280602936
40	20.458480601069965	25.902102314940024	30.812373909286183	22.82704317470383
41	21.16124690310327	25.09380061114168	31.08219637977824	22.66275610597682
42	20.230157323908198	26.75291349205609	30.350986810406194	22.665942373629516
43	20.609167746889113	26.335823284933152	29.995445968671998	23.05956299950574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	160.0
2	214.0
3	419.0
4	624.0
5	624.0
6	1047.5
7	1471.0
8	1607.5
9	1744.0
10	2634.5
11	3525.0
12	3525.0
13	6804.0
14	10083.0
15	15772.0
16	21461.0
17	20706.5
18	19952.0
19	19952.0
20	22319.0
21	24686.0
22	21338.5
23	17991.0
24	21149.5
25	24308.0
26	24308.0
27	27710.0
28	31112.0
29	33884.5
30	36657.0
31	40748.5
32	44840.0
33	44840.0
34	50641.0
35	56442.0
36	61822.5
37	67203.0
38	71511.5
39	75820.0
40	75820.0
41	78687.0
42	81554.0
43	85183.5
44	88813.0
45	90663.0
46	92513.0
47	92513.0
48	107466.5
49	122420.0
50	112962.0
51	103504.0
52	94539.0
53	85574.0
54	85574.0
55	77464.5
56	69355.0
57	61164.5
58	52974.0
59	48525.0
60	44076.0
61	44076.0
62	39403.5
63	34731.0
64	29686.0
65	24641.0
66	20794.0
67	16947.0
68	16947.0
69	14344.0
70	11741.0
71	9804.5
72	7868.0
73	6481.5
74	5095.0
75	5095.0
76	4042.5
77	2990.0
78	2336.0
79	1682.0
80	1293.5
81	905.0
82	905.0
83	710.5
84	516.0
85	403.0
86	290.0
87	236.5
88	183.0
89	183.0
90	140.0
91	97.0
92	63.5
93	30.0
94	27.5
95	25.0
96	25.0
97	15.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1286772.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.37709232763803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26714722415737	43.895841303806385
2	12.215454088876923	13.529126578075218
3	3.755074531120106	6.238353270210006
4	1.6013032427007774	3.5470207006234844
5	0.8019542974677039	2.220494858670756
6	0.47486466152764395	1.5777974522729352
7	0.32101401581174216	1.244377595445089
8	0.2212582152453988	0.9802109291114285
9	0.16997682967883546	0.8471540331615669
>10	1.00189725817095	10.636649703577044
>50	0.10337742183785371	3.964552295271024
>100	0.06105137155289731	6.089025482704123
>500	0.0033761051111441615	1.219517223344924
>1k	0.0018287236018697539	1.8110083940161479
>5k	1.4067104629767339E-4	0.6178212135642666
>10k+	2.8134209259534677E-4	1.5810489661455496
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10269	0.7980434762335519	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10027	0.7792367256981035	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7931	0.6163485061844678	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3828	0.297488599378911	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2473	0.1921863391494375	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2287	0.1777315639445061	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2267	0.17617728704075003	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2050	0.15931338263499673	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1806	0.14035120440917273	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1667	0.12954897992806808	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1352	0.10506911869391003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.771384518780328E-5	0.0
9	0.0	0.0	0.0	7.771384518780328E-5	0.0
10	0.0	0.0	0.0	2.3314153556340983E-4	0.0
11	0.0	0.0	0.0	2.3314153556340983E-4	0.0
12	0.0	0.0	0.0	5.43996916314623E-4	0.0
13	0.0	0.0	0.0	8.54852297065836E-4	0.0
14	0.0	0.0	0.0	0.0010102799874414426	0.0
15	0.0	0.0	0.0	0.0011657076778170492	0.0
16	0.0	0.0	0.0	0.0013211353681926558	0.0
17	0.0	0.0	0.0	0.0018651322845072787	0.0
18	0.0	0.0	0.0	0.0023314153556340984	0.0
19	7.771384518780328E-5	0.0	0.0	0.0027199845815731147	0.0
20	7.771384518780328E-5	0.0	0.0	0.0040411199497657705	0.0
21	7.771384518780328E-5	0.0	0.0	0.007460529138029115	0.0
22	7.771384518780328E-5	0.0	0.0	0.012123359849297312	0.0
23	7.771384518780328E-5	0.0	0.0	0.014998772121246033	0.0
24	7.771384518780328E-5	0.0	0.0	0.019117605916199605	0.0
25	7.771384518780328E-5	0.0	0.0	0.02463528892453364	0.0
26	7.771384518780328E-5	0.0	0.0	0.03419409188263344	0.0
27	7.771384518780328E-5	0.0	0.0	0.09084748502454203	0.0
28	7.771384518780328E-5	0.0	0.0	0.2036879882372324	0.0
29	7.771384518780328E-5	0.0	0.0	0.33075012511929075	0.0
30	7.771384518780328E-5	0.0	0.0	0.525811876540677	0.0
31	7.771384518780328E-5	0.0	0.0	0.795245777806791	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2490	0.0	29.050201	1
ACGTTTA	80	9.786163E-10	25.437502	26
CGTTTAT	105	2.2600943E-8	19.38095	27
TGGCGTA	60	9.237634E-4	18.5	5
GCGACTA	50	0.0070353295	18.499998	35
TAAGCCG	100	2.8755858E-7	18.499998	36
CTGCGTA	85	2.723526E-5	17.411764	9
TAAAACG	65	0.001580073	17.076923	4
CGAGCTA	65	0.001580073	17.076923	22
ACGACGC	65	0.001580073	17.076923	28
GTATCAA	4330	0.0	16.705544	2
TATACGG	90	4.4466207E-5	16.444445	2
TTGGCGT	70	0.0025927192	15.857143	4
TCACGTT	130	2.5894587E-7	15.653846	24
GTCTCAC	135	3.9729093E-7	15.074075	1
ACACCGC	75	0.0041047605	14.8	6
AATCGGC	75	0.0041047605	14.8	25
AGTACCG	100	1.0935253E-4	14.799999	5
ATTGGAC	280	0.0	14.535715	3
TCAAGAC	275	0.0	14.127274	3
>>END_MODULE