FastQCFastQC Report
Wed 25 May 2016
SRR2088097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402385
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30910.7681697876411894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29400.73064353790524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21630.5375448886017123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11320.28132261391453456No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA5360.1332057606521118No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG5350.13295724244193993No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG4490.11158467636716081No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG4390.1090994942654423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA303.5953577E-430.8333341
CTAGTAC250.005492151729.5999983
GGTATCA9100.025.208791
CTAGGCG450.003821551820.55555522
CTAGCAC450.003821551820.5555553
CTGTACC656.88789E-519.9230774
TATTCTC851.2411965E-619.5882365
GAGTGGG902.1451506E-618.57
GCCTAAA609.222599E-418.51
TCCGGCG500.007027132418.49999817
TAGGCGC500.007027132418.49999823
GTCGGAA500.007027132418.49999811
CATACTG852.7159987E-517.4117645
TTATACT752.0630617E-417.2666664
GTACTAG752.0630617E-417.2666661
ATGAGTG650.001577522517.0769235
TAGCACA1005.858714E-616.654
GTATCAA14100.016.2695032
CCTTCCG700.002588568315.85714314
CGGCGGT700.002588568315.85714319
Produced by FastQC (version 0.11.5)