##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	402385
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.611561067137195	31.0	31.0	34.0	30.0	34.0
2	31.828353442598505	31.0	31.0	34.0	30.0	34.0
3	31.90125625955242	31.0	31.0	34.0	30.0	34.0
4	35.61723473787542	37.0	35.0	37.0	33.0	37.0
5	35.47293263913913	37.0	35.0	37.0	33.0	37.0
6	35.49186475639002	37.0	35.0	37.0	33.0	37.0
7	35.38001913590218	37.0	35.0	37.0	33.0	37.0
8	35.38071995725487	37.0	35.0	37.0	33.0	37.0
9	36.971099817339116	39.0	37.0	39.0	33.0	39.0
10	36.792052387638705	39.0	37.0	39.0	32.0	39.0
11	36.85033487828821	39.0	37.0	39.0	33.0	39.0
12	36.744558072492765	39.0	35.0	39.0	32.0	39.0
13	36.76795854716254	39.0	35.0	39.0	32.0	39.0
14	37.80431179094648	40.0	37.0	41.0	32.0	41.0
15	37.81360388682481	40.0	37.0	41.0	32.0	41.0
16	37.69388272425662	39.0	37.0	41.0	32.0	41.0
17	37.66723908694409	39.0	37.0	41.0	32.0	41.0
18	37.64615480199311	39.0	37.0	41.0	32.0	41.0
19	37.67884488735912	39.0	37.0	41.0	32.0	41.0
20	37.589552294444374	39.0	36.0	41.0	32.0	41.0
21	37.503348782882064	39.0	36.0	41.0	32.0	41.0
22	37.39290480509959	39.0	36.0	41.0	32.0	41.0
23	37.20613839979124	39.0	36.0	41.0	31.0	41.0
24	37.06349143233471	39.0	36.0	41.0	31.0	41.0
25	36.942875604209895	39.0	36.0	40.0	31.0	41.0
26	37.07608136486201	39.0	36.0	41.0	31.0	41.0
27	36.99630204903264	39.0	36.0	41.0	31.0	41.0
28	36.92572784770804	39.0	36.0	41.0	31.0	41.0
29	36.83255340035041	39.0	36.0	40.0	30.0	41.0
30	36.671777029461836	39.0	35.0	40.0	30.0	41.0
31	36.535872361047254	39.0	35.0	40.0	30.0	41.0
32	36.36980752264622	38.0	35.0	40.0	30.0	41.0
33	36.23410415398188	38.0	35.0	40.0	30.0	41.0
34	36.10107235607689	38.0	35.0	40.0	29.0	41.0
35	35.9391229792363	38.0	35.0	40.0	29.0	41.0
36	35.84096822694683	38.0	35.0	40.0	28.0	41.0
37	35.68753556916883	38.0	35.0	40.0	27.0	41.0
38	35.62164593610597	38.0	34.0	40.0	27.0	41.0
39	35.56673086720429	38.0	34.0	40.0	26.0	41.0
40	35.441524907737616	38.0	34.0	40.0	26.0	41.0
41	35.34891211153497	38.0	34.0	40.0	25.0	41.0
42	35.2066528324863	38.0	34.0	40.0	25.0	41.0
43	34.87446599649589	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	2.0
17	11.0
18	19.0
19	45.0
20	161.0
21	276.0
22	486.0
23	838.0
24	1282.0
25	2118.0
26	3180.0
27	4378.0
28	6238.0
29	8144.0
30	10491.0
31	13127.0
32	16440.0
33	20615.0
34	25777.0
35	31611.0
36	40581.0
37	54876.0
38	75842.0
39	85843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.71196739441083	22.361171514842752	14.02736185493992	23.899499235806505
2	18.662226474644932	23.487456043341577	35.27641437926364	22.573903102749853
3	18.736781937696485	25.08741628042795	32.731836425314064	23.443965356561502
4	13.98859301415311	17.618698510133328	36.97155709084583	31.421151384867724
5	12.686109074642443	38.43483231233768	35.57488474968003	13.304173863339836
6	31.179591684580686	38.702237906482594	14.797519788262484	15.32065062067423
7	25.43285659256682	33.3168482920586	22.7597947239584	18.49050039141618
8	25.31481044273519	35.763510071200464	20.003230736732235	18.918448749332107
9	24.54539806404314	15.180237831927135	22.44268548777912	37.831678616250606
10	16.074903388545795	28.38077960162531	34.52091902034122	21.02339798948768
11	32.80490077910459	23.501124544901028	23.626626241037812	20.06734843495657
12	19.944331920921503	27.74009965580228	31.42984952222374	20.885718901052474
13	29.41237869204866	22.170061011220596	26.425935360413533	21.99162493631721
14	20.115063931309567	23.746909054760987	27.9968189669098	28.141208047019646
15	22.777439516880598	30.39974154106142	25.02677783714602	21.79604110491196
16	21.771934838525294	29.23394261714527	26.65407507735129	22.340047466978145
17	21.224449221516707	28.450861736893774	27.853423959640644	22.47126508194888
18	21.730929333846937	27.37080159548691	29.39622500838749	21.502044062278664
19	21.99187345452738	26.902096251102797	30.334629769996397	20.771400524373423
20	22.49959615790847	26.55019446549946	30.042620873044473	20.907588503547597
21	21.7321719248978	27.05493495035849	30.102016725275544	21.11087639946817
22	21.342495371348335	27.29028169539123	29.97129614672515	21.395926786535284
23	21.802751096586604	27.115076357220076	30.200181418293425	20.881991127899894
24	21.58604321731675	27.59670464853312	29.07041763485219	21.746834499297936
25	21.68545050138549	27.559426917007347	29.63132323521006	21.123799346397107
26	21.159834486872025	28.231668675522197	29.475750835642483	21.132746001963294
27	21.076580886464455	27.869080606881468	29.313468444400264	21.740870062253812
28	20.940889943710626	27.97072455484176	29.60050697714875	21.487878524298868
29	21.288566919741044	27.650136063720073	29.384793170719586	21.676503845819305
30	21.45358301129515	27.779614051219603	29.988443903227008	20.778359034258234
31	21.781130012301652	27.6516271729811	29.79981858170657	20.767424233010672
32	21.358152018589163	27.598941312424667	29.61367844228786	21.42922822669831
33	21.24880400611355	27.466729624613244	29.9496750624402	21.334791306833008
34	20.845210432794463	27.254495073126485	30.48572884178088	21.414565652298172
35	20.836512295438446	27.97668899188588	29.7836648980454	21.40313381463027
36	21.803993687637462	27.132472631932103	29.597524758626687	21.466008921803745
37	21.163562260024605	26.98783503361209	30.061756775227703	21.786845931135606
38	21.017682070653727	26.382196155423287	30.85875467524883	21.741367098674154
39	20.522136759571058	26.43189979745766	31.336655193409296	21.709308249561985
40	20.12873243286902	26.361817662189196	31.355542577382355	22.153907327559427
41	20.49007791045889	25.757918411471604	31.50191980317358	22.25008387489593
42	19.707991103048077	27.040272375958345	30.830423599289237	22.421312921704338
43	19.998508890738968	26.50421859661767	30.814269915628067	22.683002597015296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	69.0
1	80.5
2	92.0
3	147.0
4	202.0
5	202.0
6	331.0
7	460.0
8	506.0
9	552.0
10	915.0
11	1278.0
12	1278.0
13	2266.0
14	3254.0
15	5014.5
16	6775.0
17	6490.0
18	6205.0
19	6205.0
20	7230.5
21	8256.0
22	7452.5
23	6649.0
24	7685.5
25	8722.0
26	8722.0
27	9848.5
28	10975.0
29	11905.5
30	12836.0
31	14479.0
32	16122.0
33	16122.0
34	17966.0
35	19810.0
36	21599.0
37	23388.0
38	24494.5
39	25601.0
40	25601.0
41	26354.5
42	27108.0
43	27804.5
44	28501.0
45	28873.5
46	29246.0
47	29246.0
48	32035.0
49	34824.0
50	32182.0
51	29540.0
52	26815.5
53	24091.0
54	24091.0
55	21892.0
56	19693.0
57	17686.0
58	15679.0
59	14180.0
60	12681.0
61	12681.0
62	11021.0
63	9361.0
64	8227.5
65	7094.0
66	5876.5
67	4659.0
68	4659.0
69	3902.5
70	3146.0
71	2637.5
72	2129.0
73	1785.5
74	1442.0
75	1442.0
76	1133.0
77	824.0
78	651.5
79	479.0
80	383.0
81	287.0
82	287.0
83	224.5
84	162.0
85	127.0
86	92.0
87	69.5
88	47.0
89	47.0
90	46.0
91	45.0
92	26.0
93	7.0
94	4.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	402385.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.98559646752943
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.66153149791315	57.380887817097715
2	8.86775894347299	11.880242443176137
3	2.322768816238683	4.667761638357763
4	0.9230276917262186	2.473182419453105
5	0.469853921050355	1.5736722577082756
6	0.3232257097885522	1.2990880178296038
7	0.22535875976647154	1.056705365949784
8	0.16963801938750017	0.9090643129793616
9	0.13582753188553004	0.8188639416058142
>10	0.8222394589077437	10.5373492760264
>50	0.05461857797702764	2.38808324746656
>100	0.021921742173211838	2.4274075982974814
>500	7.431099041766725E-4	0.2665593702221473
>1k	0.001486219808353345	2.321132293829828
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3091	0.7681697876411894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2940	0.73064353790524	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2163	0.5375448886017123	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1132	0.28132261391453456	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	536	0.1332057606521118	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	535	0.13295724244193993	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	449	0.11158467636716081	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	439	0.1090994942654423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	2.4851821017185034E-4	0.0
14	0.0	0.0	0.0	2.4851821017185034E-4	0.0
15	0.0	0.0	0.0	2.4851821017185034E-4	0.0
16	0.0	0.0	0.0	2.4851821017185034E-4	0.0
17	0.0	0.0	0.0	9.940728406874013E-4	0.0
18	0.0	0.0	0.0	0.0014911092610311021	0.0
19	0.0	0.0	0.0	0.0017396274712029524	0.0
20	0.0	0.0	0.0	0.0027337003118903538	0.0
21	0.0	0.0	0.0	0.0049703642034370065	0.0
22	0.0	0.0	0.0	0.00670999167463996	0.0
23	0.0	0.0	0.0	0.009443691986530313	0.0
24	0.0	0.0	0.0	0.01441405618996732	0.0
25	0.0	0.0	0.0	0.017644792922201374	0.0
26	0.0	0.0	0.0	0.02932514880027834	0.0
27	0.0	0.0	0.0	0.08772692819066318	0.0
28	0.0	0.0	0.0	0.1861401394187159	0.0
29	0.0	0.0	0.0	0.31164183555550035	0.0
30	0.0	0.0	0.0	0.49827901139455993	0.0
31	0.0	0.0	0.0	0.7681697876411894	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGA	30	3.5953577E-4	30.833334	1
CTAGTAC	25	0.0054921517	29.599998	3
GGTATCA	910	0.0	25.20879	1
CTAGGCG	45	0.0038215518	20.555555	22
CTAGCAC	45	0.0038215518	20.555555	3
CTGTACC	65	6.88789E-5	19.923077	4
TATTCTC	85	1.2411965E-6	19.588236	5
GAGTGGG	90	2.1451506E-6	18.5	7
GCCTAAA	60	9.222599E-4	18.5	1
TCCGGCG	50	0.0070271324	18.499998	17
TAGGCGC	50	0.0070271324	18.499998	23
GTCGGAA	50	0.0070271324	18.499998	11
CATACTG	85	2.7159987E-5	17.411764	5
TTATACT	75	2.0630617E-4	17.266666	4
GTACTAG	75	2.0630617E-4	17.266666	1
ATGAGTG	65	0.0015775225	17.076923	5
TAGCACA	100	5.858714E-6	16.65	4
GTATCAA	1410	0.0	16.269503	2
CCTTCCG	70	0.0025885683	15.857143	14
CGGCGGT	70	0.0025885683	15.857143	19
>>END_MODULE