FastQCFastQC Report
Wed 25 May 2016
SRR2088096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865442
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT269561.4450194645558534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT262111.4050825488007668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT210221.1269179100717148No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100070.5364412294780541No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG80800.43314131449811893No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG80560.43185475613822355No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA76270.4088575254550932No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA70270.3766935664577082No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA70170.37615750047441837No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG69440.37224421879640324No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA41340.22160967749198313No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38550.20665343655819907No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37930.20332982746180261No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34720.18612210939820162No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32700.17529357653574862No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32610.17481111715078784No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA32530.17438226436415605No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG28900.1549230691707381No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG28030.15025929511611724No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.14720371901136567No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG27300.14634601343810205No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC25290.13557108717397806No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT25030.13417731561742471No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC23970.12849501619455336No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23950.1283878029978954No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23460.12576107967977562No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT20570.1102687727627018No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA18930.1014772906367499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62450.030.186551
TCTAACG705.1047373E-621.1428572
CGACGAG1103.855348E-818.524
TATACCG1601.8189894E-1218.55
GCGTCCC609.239758E-418.511
GTATTGG6700.018.223881
GTATCAA108350.017.4328562
ACGCCTA904.4483466E-516.4444456
ATTGGAC7200.016.18753
CAATGCG1151.2431337E-616.08695619
GCGACGA1403.4766344E-815.85714223
GTATAAC1059.349262E-615.8571421
CTAGTAC2700.015.7592593
CCTAGTA1701.4879333E-915.2352942
CGCCGGA1752.240995E-914.814
CTAGGCG750.00410568114.812
CGTGCGG1406.0001184E-714.53571410
CGACCGC2059.276846E-1114.43902510
CCGACCG2201.8189894E-1114.2954549
TTGGACC11700.014.230774
Produced by FastQC (version 0.11.5)