##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088096_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1865442
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.574802111242267	31.0	31.0	34.0	30.0	34.0
2	31.771209182595868	31.0	31.0	34.0	30.0	34.0
3	31.831330590819764	31.0	31.0	34.0	30.0	34.0
4	35.52723483228104	37.0	35.0	37.0	33.0	37.0
5	35.441062225467206	37.0	35.0	37.0	33.0	37.0
6	35.48242936526571	37.0	35.0	37.0	33.0	37.0
7	35.33453358506992	37.0	35.0	37.0	32.0	37.0
8	35.38692170541888	37.0	35.0	37.0	33.0	37.0
9	36.91437686081905	39.0	37.0	39.0	33.0	39.0
10	36.77162731406283	39.0	37.0	39.0	32.0	39.0
11	36.81717952099288	39.0	37.0	39.0	32.0	39.0
12	36.68823420937236	39.0	35.0	39.0	32.0	39.0
13	36.71776876472171	39.0	35.0	39.0	32.0	39.0
14	37.66180079573635	39.0	36.0	41.0	32.0	41.0
15	37.67125592754961	39.0	36.0	41.0	32.0	41.0
16	37.542674604731744	39.0	36.0	41.0	32.0	41.0
17	37.608382892633486	39.0	36.0	41.0	32.0	41.0
18	37.60452482575175	39.0	36.0	41.0	32.0	41.0
19	37.65391044052831	39.0	37.0	41.0	32.0	41.0
20	37.58021369734358	39.0	36.0	41.0	32.0	41.0
21	37.49654237440778	39.0	36.0	41.0	32.0	41.0
22	37.385736463529824	39.0	36.0	41.0	31.0	41.0
23	37.1594731972369	39.0	36.0	41.0	31.0	41.0
24	37.002428378904305	39.0	36.0	41.0	31.0	41.0
25	36.85202488203868	39.0	35.0	40.0	30.0	41.0
26	37.00657806568095	39.0	36.0	40.0	31.0	41.0
27	36.94067250549735	39.0	35.0	41.0	31.0	41.0
28	36.84289514227727	39.0	35.0	41.0	30.0	41.0
29	36.743856951864494	39.0	35.0	40.0	30.0	41.0
30	36.490537363262966	39.0	35.0	40.0	30.0	41.0
31	36.236549836446265	38.0	35.0	40.0	30.0	41.0
32	36.069324589025015	38.0	35.0	40.0	30.0	41.0
33	35.87254173541713	38.0	35.0	40.0	29.0	41.0
34	35.72734343924925	38.0	35.0	40.0	28.0	41.0
35	35.50495753821346	38.0	34.0	40.0	27.0	41.0
36	35.39512512316116	38.0	34.0	40.0	26.0	41.0
37	35.189380318444634	38.0	34.0	40.0	25.0	41.0
38	35.066098007871595	38.0	34.0	40.0	25.0	41.0
39	34.92776618088367	38.0	34.0	40.0	24.0	41.0
40	34.70230594143372	38.0	33.0	40.0	23.0	41.0
41	34.51214939944528	38.0	33.0	40.0	23.0	41.0
42	34.23511585994097	38.0	33.0	40.0	21.0	41.0
43	33.89717503948126	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	2.0
14	5.0
15	13.0
16	21.0
17	58.0
18	151.0
19	299.0
20	623.0
21	1310.0
22	2623.0
23	4514.0
24	7517.0
25	11681.0
26	17227.0
27	24170.0
28	32273.0
29	42331.0
30	53389.0
31	65578.0
32	80668.0
33	99080.0
34	122434.0
35	148737.0
36	191951.0
37	263540.0
38	338432.0
39	356813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18055774449165	22.269092258027857	15.041689851520443	22.508660145960047
2	18.69428264186182	23.87380577900573	35.61579507698443	21.816116502148017
3	19.38961382878696	24.66021457649179	34.07948357547434	21.870688019246913
4	13.609428757366887	16.45722568699536	36.32897726115312	33.60436829448463
5	11.748690122769831	39.16535598533753	35.30123155799001	13.784722333902636
6	31.56972985490838	37.633011372103766	12.86231359645596	17.934945176531887
7	24.669488518002705	34.524739981194806	21.137725000294836	19.668046500507653
8	26.80120850715273	34.95139489729512	18.779356313409906	19.468040282142248
9	23.69309793603875	14.869291031294459	22.867127468985903	38.57048356368089
10	16.198305817066412	28.036250926053985	33.56185826200975	22.20358499486985
11	32.07990385120524	22.51895261283921	24.310806768583532	21.090336767372023
12	20.8847554627804	27.82750683216096	31.16805561362937	20.11968209142927
13	31.947013093947707	21.377614527817	27.180153550740254	19.49521882749504
14	19.940582446412165	23.717596151475092	28.375044627493107	27.966776774619635
15	21.54770826431484	30.01208292726335	26.77472684757822	21.665481960843596
16	20.909628924405048	29.24781365488715	27.29224494784614	22.55031247286166
17	20.935520911397944	26.96679928939093	27.329662353479765	24.76801744573136
18	23.090184524632768	25.27728012985662	30.778603676769368	20.85393166874124
19	22.202566469501598	24.283628223230743	32.1623507994352	21.35145450783246
20	23.613599350716882	23.745632402401146	32.27696170666255	20.36380654021942
21	22.258692577952036	24.746360380006454	33.044500981536814	19.950446060504696
22	20.11753782749611	25.139511172151156	32.44196281631913	22.300988184033596
23	21.782558771594076	25.4846304521931	33.65181013400577	19.081000642207048
24	21.592845020107834	25.309765728443985	30.62834438165325	22.46904486979493
25	21.158363540651493	26.18762738267928	31.89710535090343	20.756903725765795
26	21.089264635405446	26.81536064911158	31.674530754641527	20.420843960841452
27	19.396957932758028	27.101030211606687	31.27483995750069	22.2271718981346
28	20.329551923887205	26.199742473901626	31.485835528523538	21.984870073687627
29	20.675904155690716	25.9229180001308	30.45524867564899	22.945929168529496
30	21.019576057577773	26.31987486075686	32.54445863232414	20.11609044934123
31	22.197152203070374	25.840363838704178	32.30135270890223	19.661131249323216
32	21.451162780724353	25.81833152679097	30.953736433510127	21.77676925897455
33	20.894726290069592	25.834359899691332	31.936345380880244	21.334568429358832
34	19.663811579239663	26.8146637633333	32.711550399315556	20.80997425811148
35	19.261011599395747	29.33213683405863	31.032538133053723	20.374313433491903
36	22.122746244589752	26.167417695109258	30.93031035004036	20.779525710260625
37	21.12512744968753	27.29004707731465	30.805406975933852	20.77941849706397
38	20.851519371816437	25.32037983491312	32.40143622798243	21.42666456528801
39	20.30376715009097	24.997721719571018	33.4107948679187	21.28771626241931
40	18.747782026994138	25.766761979198492	32.79598079168369	22.68947520212368
41	20.841066085142288	24.87378326423443	32.60422998946094	21.680920661162343
42	19.293389984786447	27.513747412141466	31.751938682628566	21.44092392044352
43	19.739718522473495	27.68979148105382	30.3808963237667	22.18959367270599
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	212.0
2	304.0
3	608.0
4	912.0
5	912.0
6	1773.5
7	2635.0
8	3001.0
9	3367.0
10	5616.0
11	7865.0
12	7865.0
13	15633.5
14	23402.0
15	37932.5
16	52463.0
17	49202.5
18	45942.0
19	45942.0
20	49660.5
21	53379.0
22	42337.5
23	31296.0
24	34699.0
25	38102.0
26	38102.0
27	41138.5
28	44175.0
29	46357.0
30	48539.0
31	52960.0
32	57381.0
33	57381.0
34	63261.5
35	69142.0
36	75592.0
37	82042.0
38	86032.0
39	90022.0
40	90022.0
41	93167.0
42	96312.0
43	100615.0
44	104918.0
45	115380.5
46	125843.0
47	125843.0
48	176729.0
49	227615.0
50	204403.0
51	181191.0
52	162371.5
53	143552.0
54	143552.0
55	122682.5
56	101813.0
57	83702.0
58	65591.0
59	58346.0
60	51101.0
61	51101.0
62	44727.0
63	38353.0
64	33154.5
65	27956.0
66	23300.5
67	18645.0
68	18645.0
69	15469.0
70	12293.0
71	10106.0
72	7919.0
73	6431.0
74	4943.0
75	4943.0
76	3875.0
77	2807.0
78	2227.0
79	1647.0
80	1253.0
81	859.0
82	859.0
83	650.5
84	442.0
85	360.5
86	279.0
87	208.5
88	138.0
89	138.0
90	113.5
91	89.0
92	60.0
93	31.0
94	22.0
95	13.0
96	13.0
97	7.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1865442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.24986674241237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28414760490668	33.75563366075636
2	12.92969031431293	11.442741468580143
3	4.485499422366321	5.954482551386323
4	2.051484202720437	3.6311161037817374
5	1.0742245627636466	2.3767146876858782
6	0.6387550622373281	1.6958895831025858
7	0.41688773673264995	1.2913058757875957
8	0.29617767753704993	1.0484658210473314
9	0.21452019036523567	0.8543240853496753
>10	1.3566540649149046	11.593655044641514
>50	0.15031132044709372	4.629195768049149
>100	0.08804653170340415	7.304014669064561
>500	0.005343371389896739	1.6979328144231025
>1k	0.007043535013954793	5.795283234846963
>5k	7.286415531677372E-4	2.404791321272534
>10k+	4.857610354451581E-4	4.524453310224627
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26956	1.4450194645558534	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26211	1.4050825488007668	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21022	1.1269179100717148	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10007	0.5364412294780541	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8080	0.43314131449811893	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8056	0.43185475613822355	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7627	0.4088575254550932	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7027	0.3766935664577082	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7017	0.37615750047441837	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6944	0.37224421879640324	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4134	0.22160967749198313	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3855	0.20665343655819907	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3793	0.20332982746180261	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3472	0.18612210939820162	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3270	0.17529357653574862	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3261	0.17481111715078784	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3253	0.17438226436415605	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2890	0.1549230691707381	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2803	0.15025929511611724	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2746	0.14720371901136567	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2730	0.14634601343810205	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2529	0.13557108717397806	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2503	0.13417731561742471	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2397	0.12849501619455336	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2395	0.1283878029978954	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2346	0.12576107967977562	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2057	0.1102687727627018	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1893	0.1014772906367499	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.360659832897512E-5	0.0
3	0.0	0.0	0.0	5.360659832897512E-5	0.0
4	0.0	0.0	0.0	5.360659832897512E-5	0.0
5	0.0	5.360659832897512E-5	0.0	5.360659832897512E-5	0.0
6	0.0	5.360659832897512E-5	0.0	1.0721319665795024E-4	0.0
7	0.0	5.360659832897512E-5	0.0	1.6081979498692536E-4	0.0
8	0.0	1.0721319665795024E-4	0.0	2.1442639331590048E-4	0.0
9	0.0	1.0721319665795024E-4	0.0	4.2885278663180095E-4	0.0
10	0.0	1.0721319665795024E-4	0.0	5.896725816187262E-4	0.0
11	0.0	1.0721319665795024E-4	0.0	0.0011257385649084775	0.0
12	0.0	1.0721319665795024E-4	0.0	0.0016081979498692536	0.0
13	0.0	1.0721319665795024E-4	0.0	0.0020906573348300297	0.0
14	0.0	1.0721319665795024E-4	0.0	0.0025195101214618306	0.0
15	0.0	1.0721319665795024E-4	0.0	0.003216395899738507	0.0
16	5.360659832897512E-5	1.0721319665795024E-4	0.0	0.0038596750796862085	0.0
17	5.360659832897512E-5	1.0721319665795024E-4	0.0	0.004985413644594686	0.0
18	5.360659832897512E-5	1.6081979498692536E-4	0.0	0.005735906021200337	0.0
19	5.360659832897512E-5	1.6081979498692536E-4	0.0	0.006968857782766765	0.0
20	5.360659832897512E-5	1.6081979498692536E-4	0.0	0.011042959255768875	0.0
21	5.360659832897512E-5	1.6081979498692536E-4	0.0	0.01983444138172079	0.0
22	5.360659832897512E-5	1.6081979498692536E-4	0.0	0.03050215444918684	0.0
23	5.360659832897512E-5	1.6081979498692536E-4	0.0	0.03698855284699283	0.0
24	5.360659832897512E-5	1.6081979498692536E-4	0.0	0.047173806529498104	0.0
25	5.360659832897512E-5	1.6081979498692536E-4	0.0	0.05124790800250021	0.0
26	1.0721319665795024E-4	1.6081979498692536E-4	0.0	0.06030742312009701	0.0
27	1.6081979498692536E-4	1.6081979498692536E-4	0.0	0.11412844784238803	0.0
28	1.6081979498692536E-4	1.6081979498692536E-4	0.0	0.2290073880613817	0.0
29	1.6081979498692536E-4	1.6081979498692536E-4	0.0	0.36993913506825726	0.0
30	1.6081979498692536E-4	1.6081979498692536E-4	0.0	0.6073091524689591	0.0
31	2.1442639331590048E-4	1.6081979498692536E-4	0.0	0.8959806844704901	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6245	0.0	30.18655	1
TCTAACG	70	5.1047373E-6	21.142857	2
CGACGAG	110	3.855348E-8	18.5	24
TATACCG	160	1.8189894E-12	18.5	5
GCGTCCC	60	9.239758E-4	18.5	11
GTATTGG	670	0.0	18.22388	1
GTATCAA	10835	0.0	17.432856	2
ACGCCTA	90	4.4483466E-5	16.444445	6
ATTGGAC	720	0.0	16.1875	3
CAATGCG	115	1.2431337E-6	16.086956	19
GCGACGA	140	3.4766344E-8	15.857142	23
GTATAAC	105	9.349262E-6	15.857142	1
CTAGTAC	270	0.0	15.759259	3
CCTAGTA	170	1.4879333E-9	15.235294	2
CGCCGGA	175	2.240995E-9	14.8	14
CTAGGCG	75	0.004105681	14.8	12
CGTGCGG	140	6.0001184E-7	14.535714	10
CGACCGC	205	9.276846E-11	14.439025	10
CCGACCG	220	1.8189894E-11	14.295454	9
TTGGACC	1170	0.0	14.23077	4
>>END_MODULE