FastQCFastQC Report
Wed 25 May 2016
SRR2088095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2773716
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT330001.1897396849569313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT316351.1405277252609858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237680.8569009949107984No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118980.4289552355035627No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG71720.25857009153063976No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA67860.24465374248841626No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG62100.2238873770782589No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG58340.2103315552132951No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50800.1831478060479155No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA48440.17463936466458715No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA41760.15055614922364077No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37880.13656769474596533No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.1303666272970989No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA34330.12376897995324684No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32480.11709922717394283No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA31220.11255658474047092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76150.029.0072211
TAGACCG450.003827151620.5555555
TATCGGT1103.857531E-818.513
TATACCG2300.017.6956525
GTATTGG5800.017.2241381
GTATCAA128750.017.1565042
CTGTGCG1851.8189894E-1116.09
TTGGACC10800.015.9305564
AGCCGTC2600.015.65384612
ATTGGAC6600.015.4166673
CGCCGGA2400.015.41666714
CGAATTA2600.014.94230715
CCGAATT2750.014.814
GGACCCT10200.014.6911766
GCCGTCA2900.014.67241413
CTAGTAC2650.014.66037753
TTAACGG1651.6478225E-814.57575735
ATCGGTC908.2792144E-414.38888814
TAAGCCG2750.014.12727410
TAGGTCG1458.9222704E-714.03448321
Produced by FastQC (version 0.11.5)