##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2773716
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52167525442403	31.0	31.0	34.0	30.0	34.0
2	31.72254369228861	31.0	31.0	34.0	30.0	34.0
3	31.78511029968461	31.0	31.0	34.0	30.0	34.0
4	35.51272985410186	37.0	35.0	37.0	33.0	37.0
5	35.38676237942168	37.0	35.0	37.0	33.0	37.0
6	35.419294549261714	37.0	35.0	37.0	33.0	37.0
7	35.28472093033317	37.0	35.0	37.0	32.0	37.0
8	35.30004694063848	37.0	35.0	37.0	32.0	37.0
9	36.8502690974851	39.0	37.0	39.0	33.0	39.0
10	36.68152254953283	39.0	35.0	39.0	32.0	39.0
11	36.75568154778644	39.0	35.0	39.0	32.0	39.0
12	36.63456496627629	39.0	35.0	39.0	32.0	39.0
13	36.67208539014088	39.0	35.0	39.0	32.0	39.0
14	37.63833572002325	39.0	36.0	41.0	32.0	41.0
15	37.666090544237406	39.0	36.0	41.0	32.0	41.0
16	37.53874080835961	39.0	36.0	41.0	32.0	41.0
17	37.562432851813234	39.0	36.0	41.0	32.0	41.0
18	37.55238315674712	39.0	36.0	41.0	32.0	41.0
19	37.58877044369358	39.0	37.0	41.0	32.0	41.0
20	37.513808190889044	39.0	36.0	41.0	32.0	41.0
21	37.430218883259855	39.0	36.0	41.0	32.0	41.0
22	37.340388850192305	39.0	36.0	41.0	31.0	41.0
23	37.144575003352905	39.0	36.0	40.0	31.0	41.0
24	37.007427580905905	39.0	36.0	40.0	31.0	41.0
25	36.87160365372662	39.0	36.0	40.0	30.0	41.0
26	36.97421942260851	39.0	36.0	40.0	31.0	41.0
27	36.888352304273404	39.0	35.0	40.0	31.0	41.0
28	36.79305740025295	39.0	35.0	40.0	30.0	41.0
29	36.677631379708664	39.0	35.0	40.0	30.0	41.0
30	36.471030199198474	39.0	35.0	40.0	30.0	41.0
31	36.28969764748806	38.0	35.0	40.0	30.0	41.0
32	36.12638208093402	38.0	35.0	40.0	30.0	41.0
33	35.962528607831516	38.0	35.0	40.0	29.0	41.0
34	35.825868257600995	38.0	35.0	40.0	29.0	41.0
35	35.641100242418474	38.0	34.0	40.0	27.0	41.0
36	35.526123799264234	38.0	34.0	40.0	27.0	41.0
37	35.35690532123692	38.0	34.0	40.0	26.0	41.0
38	35.24648558107607	38.0	34.0	40.0	25.0	41.0
39	35.15256031980203	38.0	34.0	40.0	25.0	41.0
40	34.973335049442696	38.0	34.0	40.0	24.0	41.0
41	34.83628785355098	38.0	33.0	40.0	24.0	41.0
42	34.64078081533942	38.0	33.0	40.0	23.0	41.0
43	34.28018730107913	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	6.0
15	14.0
16	33.0
17	67.0
18	208.0
19	506.0
20	974.0
21	1975.0
22	3745.0
23	6534.0
24	10714.0
25	16772.0
26	24442.0
27	34495.0
28	46951.0
29	61774.0
30	78578.0
31	97911.0
32	120408.0
33	149080.0
34	183306.0
35	223591.0
36	285066.0
37	380213.0
38	499655.0
39	546693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.50811835097753	22.551984413689073	14.875135017427885	23.06476221790551
2	18.6147392162716	23.810945316679863	35.29788197493903	22.2764334921095
3	19.071959782472323	24.864910466680797	33.48233921569476	22.580790535152122
4	13.813346427680411	17.022975675952402	36.56253920733052	32.60113868903666
5	12.222376047151187	38.963650207879965	35.21279756110575	13.601176183863092
6	31.301834794910512	38.16973331083644	13.989644217360391	16.53878767689266
7	24.674227642628157	34.03499132571611	22.177793256411256	19.112987775244473
8	26.219555282516303	35.36317344674077	19.510829515350526	18.906441755392407
9	24.181783571209163	14.827040691981445	22.572606568228327	38.418569168581065
10	16.190266054635728	28.40074470493735	33.91443103764048	21.494558202786443
11	32.49510764620459	23.132216852770796	23.73891198666338	20.633763514361238
12	20.163708180650076	27.96746314330667	31.351515439936893	20.517313236106364
13	30.682268840789757	21.8292355814366	26.896661374127707	20.591834203645938
14	19.832275546595255	23.646400712978547	28.53662739804652	27.984696342379685
15	22.05034689924996	30.291853960535253	26.058471739716683	21.599327400498105
16	21.212373581145293	29.050594941947917	27.331889782515585	22.405141694391205
17	20.796072849563547	27.80558644071707	28.00939245402197	23.38894825569741
18	22.11354731342358	26.407317836433148	30.372431784652793	21.106703065490485
19	21.995402557435582	25.67303213450836	31.58733626658245	20.744229041473606
20	22.670994434902493	25.2487998050269	31.489813665133703	20.590392094936902
21	21.651423577612128	26.02602429376331	31.941229743780546	20.381322384844015
22	20.433959352723928	26.264152494343328	31.60929237167756	21.692595781255182
23	21.477288950995703	26.4122570587616	32.212526444668455	19.89792754557424
24	21.309968288029488	26.36971485184496	30.431305872699294	21.889010987426254
25	21.230616256314633	26.77775951106746	31.226628825734142	20.764995406883763
26	20.9294318524319	27.492793061726577	30.986193251219664	20.59158183462186
27	20.117740965549466	27.403093900024373	30.682845684273374	21.79631945015279
28	20.40951561010572	27.06650572733474	30.982876401188875	21.541102261370668
29	20.66228121408248	26.751296816256602	30.527133996414918	22.059287973246
30	20.954885071146435	26.91021719599267	31.676134110341508	20.458763622519392
31	21.59283791130743	26.708610398469055	31.499223424460183	20.19932826576333
32	21.033119468611783	26.660516073022617	30.851536350513175	21.454828107852425
33	20.833135043385838	26.52978891854826	31.33922146319234	21.29785457487356
34	19.970032981026176	26.789404538892953	32.0729303216335	21.16763215844737
35	19.831734755829363	28.194811581286622	30.980532974536683	20.99292068834733
36	21.61821902458651	26.36812853226502	30.85950400113061	21.154148442017856
37	20.866772229024168	26.868071568971015	31.016117006932216	21.2490391950726
38	20.62979771541138	25.76182997826742	32.16367501214977	21.44469729417143
39	20.128052042819093	25.614374362768217	32.76146512476403	21.496108469648657
40	19.231781480151536	25.891151076750468	32.40274058339065	22.47432685970734
41	20.341520184474547	25.137901645301824	32.4902765820293	22.03030158819432
42	19.342102796393	27.047469892375425	31.630815844159965	21.97961146707161
43	19.68139492291208	26.91162325198398	31.000974865487308	22.406006959616633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	304.0
1	427.5
2	551.0
3	1016.0
4	1481.0
5	1481.0
6	2661.5
7	3842.0
8	4374.5
9	4907.0
10	7742.5
11	10578.0
12	10578.0
13	20389.5
14	30201.0
15	48510.5
16	66820.0
17	62728.5
18	58637.0
19	58637.0
20	66299.0
21	73961.0
22	61949.0
23	49937.0
24	56104.5
25	62272.0
26	62272.0
27	67593.5
28	72915.0
29	77247.0
30	81579.0
31	89844.5
32	98110.0
33	98110.0
34	109569.0
35	121028.0
36	130922.5
37	140817.0
38	148096.5
39	155376.0
40	155376.0
41	159607.0
42	163838.0
43	170794.0
44	177750.0
45	184899.5
46	192049.0
47	192049.0
48	236025.5
49	280002.0
50	253825.5
51	227649.0
52	204382.5
53	181116.0
54	181116.0
55	160157.0
56	139198.0
57	120152.0
58	101106.0
59	91132.5
60	81159.0
61	81159.0
62	71585.5
63	62012.0
64	54127.5
65	46243.0
66	38818.0
67	31393.0
68	31393.0
69	26537.0
70	21681.0
71	17917.5
72	14154.0
73	11641.0
74	9128.0
75	9128.0
76	7201.5
77	5275.0
78	4221.5
79	3168.0
80	2423.0
81	1678.0
82	1678.0
83	1262.5
84	847.0
85	645.5
86	444.0
87	348.0
88	252.0
89	252.0
90	211.0
91	170.0
92	108.5
93	47.0
94	37.5
95	28.0
96	28.0
97	18.0
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2773716.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.41470360128381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.50232421793093	29.612475642885073
2	13.889552712062029	11.504634174489132
3	5.76141054577127	7.158213302352838
4	2.9266514872281664	4.848256155512439
5	1.6098470794573467	3.3335669819559195
6	0.9744008147095082	2.4212712558026253
7	0.6544426729327568	1.8972484526479432
8	0.4614334515452117	1.5288103701969837
9	0.33394573193735366	1.244723715638935
>10	1.6118055960315851	12.27981299435132
>50	0.14480268293102602	4.194882484959468
>100	0.11619410438932448	9.31884440409433
>500	0.0071621857750244836	2.0271431278777134
>1k	0.005240623737820878	3.87758043527465
>5k	4.3671864481840657E-4	1.1243337384132346
>10k+	3.4937491585472524E-4	3.6282027635475793
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33000	1.1897396849569313	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31635	1.1405277252609858	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23768	0.8569009949107984	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11898	0.4289552355035627	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7172	0.25857009153063976	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6786	0.24465374248841626	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6210	0.2238873770782589	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5834	0.2103315552132951	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5080	0.1831478060479155	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4844	0.17463936466458715	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4176	0.15055614922364077	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3788	0.13656769474596533	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3616	0.1303666272970989	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3433	0.12376897995324684	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3248	0.11709922717394283	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3122	0.11255658474047092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.605271772596762E-5	0.0
3	0.0	0.0	0.0	3.605271772596762E-5	0.0
4	0.0	0.0	0.0	7.210543545193524E-5	0.0
5	0.0	0.0	0.0	7.210543545193524E-5	0.0
6	0.0	0.0	0.0	7.210543545193524E-5	0.0
7	0.0	0.0	0.0	1.0815815317790286E-4	0.0
8	3.605271772596762E-5	0.0	0.0	1.4421087090387048E-4	0.0
9	3.605271772596762E-5	0.0	0.0	2.1631630635580572E-4	0.0
10	3.605271772596762E-5	0.0	0.0	5.407907658895143E-4	0.0
11	3.605271772596762E-5	0.0	0.0	0.0010094760963270933	0.0
12	3.605271772596762E-5	0.0	0.0	0.0015502668622166076	0.0
13	3.605271772596762E-5	0.0	0.0	0.0017665831685724134	0.0
14	3.605271772596762E-5	0.0	0.0	0.0019468467572022513	0.0
15	3.605271772596762E-5	0.0	0.0	0.0028121119826254744	0.0
16	3.605271772596762E-5	0.0	0.0	0.003497113619418859	0.0
17	3.605271772596762E-5	0.0	0.0	0.004037904385308373	0.0
18	3.605271772596762E-5	0.0	0.0	0.004975275046183531	0.0
19	3.605271772596762E-5	0.0	0.0	0.00605685657796256	0.0
20	3.605271772596762E-5	0.0	0.0	0.009626075632833355	0.0
21	3.605271772596762E-5	0.0	0.0	0.014673456114468821	0.0
22	3.605271772596762E-5	0.0	0.0	0.023975057287768466	0.0
23	3.605271772596762E-5	0.0	0.0	0.029635333970745383	0.0
24	3.605271772596762E-5	0.0	0.0	0.0379635117654439	0.0
25	3.605271772596762E-5	0.0	0.0	0.04369589388387275	0.0
26	7.210543545193524E-5	0.0	0.0	0.05559329073344207	0.0
27	7.210543545193524E-5	0.0	0.0	0.13166452513523375	0.0
28	7.210543545193524E-5	0.0	0.0	0.26996275033204553	0.0
29	7.210543545193524E-5	0.0	0.0	0.43180340020391417	0.0
30	7.210543545193524E-5	0.0	0.0	0.6877776960582843	0.0
31	7.210543545193524E-5	0.0	0.0	0.9987684391624809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7615	0.0	29.007221	1
TAGACCG	45	0.0038271516	20.555555	5
TATCGGT	110	3.857531E-8	18.5	13
TATACCG	230	0.0	17.695652	5
GTATTGG	580	0.0	17.224138	1
GTATCAA	12875	0.0	17.156504	2
CTGTGCG	185	1.8189894E-11	16.0	9
TTGGACC	1080	0.0	15.930556	4
AGCCGTC	260	0.0	15.653846	12
ATTGGAC	660	0.0	15.416667	3
CGCCGGA	240	0.0	15.416667	14
CGAATTA	260	0.0	14.942307	15
CCGAATT	275	0.0	14.8	14
GGACCCT	1020	0.0	14.691176	6
GCCGTCA	290	0.0	14.672414	13
CTAGTAC	265	0.0	14.6603775	3
TTAACGG	165	1.6478225E-8	14.575757	35
ATCGGTC	90	8.2792144E-4	14.388888	14
TAAGCCG	275	0.0	14.127274	10
TAGGTCG	145	8.9222704E-7	14.034483	21
>>END_MODULE