FastQCFastQC Report
Wed 25 May 2016
SRR2088094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090903
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160561.4718082175958815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT159051.4579664736461444No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104670.9594803570986604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56050.5137945353528224No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG36820.33751855114524393No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA34240.3138684190986733No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG32160.29480164597585673No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA26990.24740971470424045No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG25960.23796799532130722No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA23970.21972622680476633No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.18040100723895708No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA19520.17893433238335582No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18770.17205929399772482No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16120.14776749170182865No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16060.1472174886309782No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13470.12347568940593251No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA13180.1208173412301552No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12870.11797565869742772No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12390.11357563413062391No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG11990.1099089469916207No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.10752560035126864No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC11560.10596725831719227No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG11380.10431724910464084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.028.4035341
GTATTGG1700.020.6764721
TTCCGTC656.901716E-519.9230797
ATTGGAC2100.018.5000023
TGCGAGA701.2191252E-418.527
TGTACTA701.2191252E-418.55
TTGGACC4750.018.3052644
AATGCGT752.0671441E-417.2666686
GTATCAA60750.017.2362142
CGAACTA650.001579863717.07692524
GGACCCT5100.017.0490196
GACCCTC5050.016.8514867
TGGACCC4950.016.818185
CTAATAC1452.9776857E-916.5862063
GCGAACT700.002592380415.85714223
ATGCGTT700.002592380415.8571427
TACCCCG957.0591464E-515.5789485
TATAGAC855.363838E-415.2352943
TGCGTTA855.363838E-415.2352948
CGTTAAC750.00410422714.80000110
Produced by FastQC (version 0.11.5)