##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090903
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.090414088145327	31.0	31.0	33.0	30.0	34.0
2	31.307526883691768	31.0	31.0	34.0	30.0	34.0
3	31.31730868830684	31.0	31.0	34.0	28.0	34.0
4	35.10162956743175	35.0	35.0	37.0	32.0	37.0
5	34.94450102346405	35.0	35.0	37.0	32.0	37.0
6	35.01791726670474	36.0	35.0	37.0	32.0	37.0
7	34.860905140053696	35.0	35.0	37.0	32.0	37.0
8	34.91367976804537	36.0	35.0	37.0	32.0	37.0
9	36.36752671869085	38.0	35.0	39.0	32.0	39.0
10	36.14044236746989	38.0	35.0	39.0	31.0	39.0
11	36.251519154315275	38.0	35.0	39.0	32.0	39.0
12	36.08736065443032	38.0	35.0	39.0	31.0	39.0
13	36.148964665052716	38.0	35.0	39.0	32.0	39.0
14	36.959222772327145	38.0	36.0	40.0	31.0	41.0
15	37.00405260596038	38.0	36.0	40.0	31.0	41.0
16	36.828709793629685	38.0	36.0	40.0	31.0	41.0
17	36.90057777822593	38.0	36.0	40.0	31.0	41.0
18	36.90093528022198	38.0	36.0	40.0	31.0	41.0
19	36.94887171453374	39.0	36.0	40.0	31.0	41.0
20	36.8851786089139	39.0	36.0	40.0	31.0	41.0
21	36.78995566058577	39.0	36.0	40.0	30.0	41.0
22	36.68944626607499	39.0	35.0	40.0	30.0	41.0
23	36.46958803853322	38.0	35.0	40.0	30.0	41.0
24	36.30668996235229	38.0	35.0	40.0	30.0	41.0
25	36.16936061226342	38.0	34.0	40.0	29.0	41.0
26	36.22538392506025	38.0	35.0	40.0	30.0	41.0
27	36.10189265223398	38.0	35.0	40.0	29.0	41.0
28	35.98445324653063	38.0	34.0	40.0	29.0	41.0
29	35.8522728418567	38.0	34.0	40.0	29.0	41.0
30	35.599407096689625	38.0	34.0	40.0	27.0	41.0
31	35.382422635193045	38.0	34.0	40.0	27.0	41.0
32	35.20411072295153	38.0	34.0	40.0	27.0	41.0
33	35.011309896480256	38.0	33.0	40.0	26.0	41.0
34	34.844848717072004	37.0	33.0	40.0	25.0	41.0
35	34.61455601460442	37.0	33.0	40.0	25.0	41.0
36	34.4889270631761	37.0	33.0	40.0	24.0	41.0
37	34.282216659043016	37.0	33.0	40.0	24.0	41.0
38	34.12667395726293	37.0	33.0	40.0	23.0	41.0
39	33.99876615977772	37.0	33.0	40.0	22.0	41.0
40	33.76452076857429	37.0	33.0	40.0	21.0	41.0
41	33.59172355378984	37.0	32.0	40.0	19.0	41.0
42	33.33414978233628	37.0	32.0	40.0	18.0	41.0
43	32.95308748807181	37.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	1.0
14	5.0
15	11.0
16	26.0
17	55.0
18	143.0
19	306.0
20	749.0
21	1373.0
22	2449.0
23	4218.0
24	6663.0
25	9808.0
26	14258.0
27	19041.0
28	25094.0
29	32010.0
30	39691.0
31	47757.0
32	57463.0
33	69118.0
34	81821.0
35	96970.0
36	121950.0
37	153430.0
38	184627.0
39	121861.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.447231330374926	23.856108196604097	16.045972923348824	21.650687549672153
2	19.899752773619653	25.072898323682306	33.72912165426257	21.29822724843547
3	20.472397637553478	24.916055781311446	33.664679627794584	20.94686695334049
4	15.036533953981243	18.18704321099126	34.9719452600277	31.804477574999794
5	11.901241448598087	39.8502891641145	34.78778589847127	13.460683488816146
6	31.526726024220302	38.03931238616082	13.207132073154076	17.2268295164648
7	24.269802173062132	34.75194403168751	21.53518690479355	19.443066890456805
8	26.660665522049165	35.58519868402599	18.87583039005301	18.87830540387184
9	23.805874582799756	14.919108298354667	22.729151904431465	38.545865214414114
10	16.415666654138818	29.112579211900602	33.392244773366656	21.079509360593928
11	32.381797465035845	23.06942047093096	23.59485673795012	20.95392532608307
12	20.307030047584433	28.641593248895642	31.143465551015993	19.907911152503935
13	31.552667835728748	21.77104655500993	27.078392854360107	19.597892754901213
14	19.640701327249076	24.27209385252401	28.887169620030377	27.20003520019653
15	21.798913377266356	30.661662860950972	26.417105828840874	21.12231793294179
16	20.501089464416175	29.17427122301433	28.10387357996082	22.220765732608676
17	20.791216084289804	28.016239757338646	28.11249029473748	23.080053863634074
18	21.903780629441847	26.17473780895277	30.87451404937011	21.046967512235277
19	21.735938025653976	25.384841732033003	32.72683272481605	20.152387517496972
20	22.481100519477902	24.946855953279073	32.46053957134594	20.111503955897085
21	21.618054034135024	25.645634854794608	33.22531884136353	19.51099226970684
22	19.85061916595701	26.235879816995645	32.56669016402009	21.34681085302726
23	21.231126873791712	26.301513516783803	33.34861119641251	19.118748413011975
24	20.921475144902892	26.380805626164744	31.192232489964734	21.505486738967626
25	20.744374156089037	26.960233861305728	32.13594609236568	20.159445890239557
26	20.519147898575767	27.653329397755805	31.852602843699213	19.974919859969216
27	19.444716899669356	27.51738697207726	31.679810212273686	21.358085915979697
28	19.986378257278602	27.156218288885448	31.9567367584469	20.90066669538905
29	20.196937766235862	26.649298791918252	31.139707196698513	22.01405624514737
30	20.61970679336293	27.01028414075312	32.72527438278197	19.64473468310198
31	21.452136441095128	26.957942181843848	32.259421781771614	19.330499595289407
32	20.750790858582295	26.860133302410937	31.453758950154135	20.93531688885263
33	20.382563802647898	26.854541604523956	31.954261744628077	20.80863284820007
34	19.422533442478386	27.31342749997021	32.82198325607318	20.442055801478226
35	19.13689851435004	29.012570320184288	31.692918618795623	20.15761254667005
36	21.40703618928539	26.817049728527653	31.48034243191191	20.295571650275047
37	20.539498012197235	27.60566246494876	31.300399760565327	20.554439762288673
38	20.353321972714348	26.10021239285253	32.75057452404109	20.795891110392034
39	19.89608608648065	25.97792837676677	33.288385860154385	20.837599676598195
40	18.655187491463494	26.48099785223801	32.76533293977558	22.098481716522915
41	20.11223729332489	25.607959644441348	32.866716839169015	21.413086223064745
42	19.0355146149566	27.651496054186303	31.908428155390535	21.40456117546656
43	19.284666006051868	27.601354107560432	31.12779046349676	21.986189422890945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	145.0
1	175.0
2	205.0
3	323.5
4	442.0
5	442.0
6	871.0
7	1300.0
8	1628.0
9	1956.0
10	3290.5
11	4625.0
12	4625.0
13	9389.0
14	14153.0
15	23556.0
16	32959.0
17	30142.5
18	27326.0
19	27326.0
20	31092.5
21	34859.0
22	28417.0
23	21975.0
24	23982.5
25	25990.0
26	25990.0
27	27942.0
28	29894.0
29	30992.5
30	32091.0
31	34879.5
32	37668.0
33	37668.0
34	41574.0
35	45480.0
36	49513.5
37	53547.0
38	55119.5
39	56692.0
40	56692.0
41	57917.5
42	59143.0
43	61443.0
44	63743.0
45	68159.0
46	72575.0
47	72575.0
48	94718.5
49	116862.0
50	106028.0
51	95194.0
52	83940.5
53	72687.0
54	72687.0
55	63357.0
56	54027.0
57	45930.0
58	37833.0
59	33734.0
60	29635.0
61	29635.0
62	25923.0
63	22211.0
64	19084.0
65	15957.0
66	13402.5
67	10848.0
68	10848.0
69	9122.5
70	7397.0
71	5985.5
72	4574.0
73	3803.5
74	3033.0
75	3033.0
76	2435.0
77	1837.0
78	1398.5
79	960.0
80	738.5
81	517.0
82	517.0
83	402.0
84	287.0
85	215.0
86	143.0
87	109.0
88	75.0
89	75.0
90	54.5
91	34.0
92	24.0
93	14.0
94	8.5
95	3.0
96	3.0
97	4.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1090903.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.01468086697664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32070153013373	41.25840976234612
2	11.9146544414522	12.39473896824884
3	3.845742685857003	6.001052355040848
4	1.648189611583638	3.4292022661915644
5	0.8399713423401782	2.1845420654615175
6	0.47148131134790977	1.4714369966703105
7	0.329284044639055	1.1989323147541415
8	0.23064414639900951	0.9597505335024575
9	0.17492500110982956	0.8188801297544986
>10	1.0504473497486853	10.41753696900058
>50	0.10565774325409936	3.7777476895430913
>100	0.05733019913730622	5.676406974622574
>500	0.006723911837015829	2.501760666743928
>1k	0.0035389009668504363	3.4887697679933534
>5k	1.7694504834252182E-4	0.5158696393605958
>10k+	5.308351450275655E-4	3.904962900765631
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16056	1.4718082175958815	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15905	1.4579664736461444	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10467	0.9594803570986604	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5605	0.5137945353528224	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3682	0.33751855114524393	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3424	0.3138684190986733	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3216	0.29480164597585673	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2699	0.24740971470424045	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2596	0.23796799532130722	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2397	0.21972622680476633	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1968	0.18040100723895708	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1952	0.17893433238335582	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1877	0.17205929399772482	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1612	0.14776749170182865	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1606	0.1472174886309782	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1347	0.12347568940593251	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1318	0.1208173412301552	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1287	0.11797565869742772	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1239	0.11357563413062391	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1199	0.1099089469916207	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1173	0.10752560035126864	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1156	0.10596725831719227	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1138	0.10431724910464084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.166717847507982E-5	0.0
10	0.0	0.0	0.0	2.7500153542523944E-4	0.0
11	0.0	0.0	0.0	3.666687139003193E-4	0.0
12	0.0	0.0	0.0	8.250046062757184E-4	0.0
13	0.0	0.0	0.0	8.250046062757184E-4	0.0
14	0.0	0.0	0.0	9.166717847507982E-4	0.0
15	0.0	0.0	0.0	0.0013750076771261973	0.0
16	0.0	0.0	0.0	0.0021083451049268356	0.0
17	0.0	0.0	0.0	0.0031166840681527137	0.0
18	0.0	0.0	0.0	0.0036666871390031927	0.0
19	0.0	0.0	0.0	0.004491691745278911	0.0
20	0.0	0.0	0.0	0.006966705564106067	0.0
21	0.0	0.0	0.0	0.011825066023285296	0.0
22	0.0	0.0	0.0	0.019066773122816603	0.0
23	0.0	0.0	0.0	0.02520847408064695	9.166717847507982E-5
24	0.0	0.0	0.0	0.03217517964475302	9.166717847507982E-5
25	0.0	0.0	0.0	0.03611686831918145	9.166717847507982E-5
26	0.0	0.0	0.0	0.043358575418712755	9.166717847507982E-5
27	0.0	0.0	0.0	0.10165890092886352	9.166717847507982E-5
28	0.0	0.0	0.0	0.2121178509913347	9.166717847507982E-5
29	0.0	0.0	0.0	0.3425602459613733	9.166717847507982E-5
30	0.0	0.0	0.0	0.5572447779500103	9.166717847507982E-5
31	0.0	0.0	0.0	0.8172128961053365	9.166717847507982E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3680	0.0	28.403534	1
GTATTGG	170	0.0	20.676472	1
TTCCGTC	65	6.901716E-5	19.923079	7
ATTGGAC	210	0.0	18.500002	3
TGCGAGA	70	1.2191252E-4	18.5	27
TGTACTA	70	1.2191252E-4	18.5	5
TTGGACC	475	0.0	18.305264	4
AATGCGT	75	2.0671441E-4	17.266668	6
GTATCAA	6075	0.0	17.236214	2
CGAACTA	65	0.0015798637	17.076925	24
GGACCCT	510	0.0	17.049019	6
GACCCTC	505	0.0	16.851486	7
TGGACCC	495	0.0	16.81818	5
CTAATAC	145	2.9776857E-9	16.586206	3
GCGAACT	70	0.0025923804	15.857142	23
ATGCGTT	70	0.0025923804	15.857142	7
TACCCCG	95	7.0591464E-5	15.578948	5
TATAGAC	85	5.363838E-4	15.235294	3
TGCGTTA	85	5.363838E-4	15.235294	8
CGTTAAC	75	0.004104227	14.800001	10
>>END_MODULE